Therapeutic applications of electrospun nanofibers impregnated with various biological macromolecules for effective wound healing strategy – A review

Sethuram, Lakshimipriya; John, Thomas

doi:10.1016/j.biopha.2022.113996

Cited by 33 publications

(29 citation statements)

References 126 publications

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“…The immunohistochemical results of CD31 (Figure b) showed that treatment methods of all three groups induced the formation of blood microvessels from the wound tissue of the rats back at different degrees. Among them, the microvessel density of the wound bed in the film-rhEGF group and the LBL MN-rhEGF group was remarkably higher than that in the control group (Figure d), which resulted from the neovascularization accelerated by rhEGF at the injured site . Fascinatingly, the LBL MN-rhEGF patch contacted and treated wounds in the form of microneedle arrays, which can continuously release rhEGF to accelerate wound healing and greatly improve the density of capillaries at the wound.…”

Section: Results and Discussionmentioning

confidence: 97%

“…Among them, the microvessel density of the wound bed in the film-rhEGF group and the LBL MN-rhEGF group was remarkably higher than that in the control group (Figure 6d), which resulted from the neovascularization accelerated by rhEGF at the injured site. 49 Fascinatingly, the LBL MN-rhEGF patch contacted and treated wounds in the form of microneedle arrays, which can continuously release rhEGF to accelerate wound healing and greatly improve the density of capillaries at the wound. In general, this LBL MN-rhEGF patch showed a better wound healing effect; compared with the control group or the single film loaded with rhEGF, rats wound closure speed, tissue regeneration degree, and angiogenesis were entirety enhanced through our LBL MN-induced rhEGF transdermal administration patch that synergistically promoted wound healing in rats.…”

Section: The Rhegf Release Kinetics In Vitro Mediated By the Lbl Mn P...mentioning

confidence: 99%

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Layer-by-Layer Microneedle-Mediated rhEGF Transdermal Delivery for Enhanced Wound Epidermal Regeneration and Angiogenesis

Yuan

Yang

Huang

et al. 2023

ACS Appl. Mater. Interfaces

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Appropriate treatments for acute traumas tend to avoid hemorrhages, vascular damage, and infections. However, in the homeostasis-imbalanced wound microenvironment, currently developed therapies could not precisely and controllably deliver biomacromolecular drugs, which are confronted with challenges due to large molecular weight, poor biomembrane permeability, low dosage, rapid degradation, and bioactivity loss. To conquer this, we construct a simple and effective layer-by-layer (LBL) self-assembly transdermal delivery patch, bearing microneedles (MN) coated with recombinant human epidermal growth factor (LBL MN-rhEGF) for a sustained release to wound bed driven by typical electrostatic force. Pyramidal LBL MN-rhEGF patches hold so enough mechanical strength to penetrate the stratum corneum, and generated microchannels allow rhEGF direct delivery in situ. The administrable delivery of biomacromolecular rhEGF through hierarchically coated MN arrays follows the diffusion mechanism of Fick’s second law. Numerous efforts further have illustrated that finger-pressing LBL MN-rhEGF patches could not only promote cell proliferation of normal human dermal fibroblasts (NHDF) and human umbilical vein endothelial cells (HUVEC) in vitro but also take significant effects (regenerative epidermis: ∼144 μm; pro-angiogenesis: higher CD31 expression) in accelerating wound healing of mechanically injured rats, compared to the traditional dressing, which relies on passive diffusion. Our proof-of-concept features novel LBL biomacromolecular drug-delivery systems and self-administrated precision medicine modes at the point of care.

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Section: Results and Discussionmentioning

confidence: 97%

Section: The Rhegf Release Kinetics In Vitro Mediated By the Lbl Mn P...mentioning

confidence: 99%

Layer-by-Layer Microneedle-Mediated rhEGF Transdermal Delivery for Enhanced Wound Epidermal Regeneration and Angiogenesis

Yuan

Yang

Huang

et al. 2023

ACS Appl. Mater. Interfaces

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“…Some possess advantages over normal wound care strategies, such as the hyaluronan dressing, due to its role in promoting wound healing; however, others do not demonstrate any improvement over conventional wound care strategies, whilst also risking disrupting the wound bed through the repeated changing of dressings [ 15 ]. Others are also classed as bioactive or advanced wound dressings, as these are loaded with additional wound-modifying agents, such as antimicrobials and immunomodulatory moieties [ 16 , 17 ]. Negative pressure wound therapy removes wound exudate and contaminants through the use of suction, with this pressure drawing the wound edges closer together, aiding wound closure; however, this is a more costly treatment modality [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Evidence for Natural Products as Alternative Wound-Healing Therapies

et al. 2023

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Chronic, non-healing wounds represent a significant area of unmet medical need and are a growing problem for healthcare systems around the world. They affect the quality of life for patients and are an economic burden, being difficult and time consuming to treat. They are an escalating problem across the developed world due to the increasing incidence of diabetes and the higher prevalence of ageing populations. Effective treatment options are currently lacking, and in some cases chronic wounds can persist for years. Some traditional medicines are believed to contain bioactive small molecules that induce the healing of chronic wounds by reducing excessive inflammation, thereby allowing re-epithelisation to occur. Furthermore, many small molecules found in plants are known to have antibacterial properties and, although they lack the therapeutic selectivity of antibiotics, they are certainly capable of acting as topical antiseptics when applied to infected wounds. As these molecules act through mechanisms of action distinct from those of clinically used antibiotics, they are often active against antibiotic resistant bacteria. Although there are numerous studies highlighting the effects of naturally occurring small molecules in wound-healing assays in vitro, only evidence from well conducted clinical trials can allow these molecules or the remedies that contain them to progress to the clinic. With this in mind, we review wound-healing natural remedies that have entered clinical trials over a twenty-year period to the present. We examine the bioactive small molecules likely to be in involved and, where possible, their mechanisms of action.

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“…Biopolymers are a good substitute for traditional wound healing agents due to their benefits such as biocompatibility, biodegradability, bioactivity, bioresorptivity, no-toxicity, enhanced antithrombin activity, and anticoagulant activity, protection from mechanical stress, drying, infections, and radiation, and effective gelling and swelling ability [ 15 , 16 ]. So, various biopolymers-based dressings, such as creams, films, powders, sponges, electrospun fibers, and hydrogels were developed according to the requirements for the healing process [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

New Smart Bioactive and Biomimetic Chitosan-Based Hydrogels for Wounds Care Management

et al. 2023

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Wound management represents a continuous challenge for health systems worldwide, considering the growing incidence of wound-related comorbidities, such as diabetes, high blood pressure, obesity, and autoimmune diseases. In this context, hydrogels are considered viable options since they mimic the skin structure and promote autolysis and growth factor synthesis. Unfortunately, hydrogels are associated with several drawbacks, such as low mechanical strength and the potential toxicity of byproducts released after crosslinking reactions. To overcome these aspects, in this study new smart chitosan (CS)-based hydrogels were developed, using oxidized chitosan (oxCS) and hyaluronic acid (oxHA) as nontoxic crosslinkers. Three active product ingredients (APIs) (fusidic acid, allantoin, and coenzyme Q10), with proven biological effects, were considered for inclusion in the 3D polymer matrix. Therefore, six API-CS-oxCS/oxHA hydrogels were obtained. The presence of dynamic imino bonds in the hydrogels’ structure, which supports their self-healing and self-adapting properties, was confirmed by spectral methods. The hydrogels were characterized by SEM, swelling degree, pH, and the internal organization of the 3D matrix was studied by rheological behavior. Moreover, the cytotoxicity degree and the antimicrobial effects were also investigated. In conclusion, the developed API-CS-oxCS/oxHA hydrogels have real potential as smart materials in wound management, based on their self-healing and self-adapting properties, as well as on the benefits of APIs.

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Therapeutic applications of electrospun nanofibers impregnated with various biological macromolecules for effective wound healing strategy – A review

Cited by 33 publications

References 126 publications

Layer-by-Layer Microneedle-Mediated rhEGF Transdermal Delivery for Enhanced Wound Epidermal Regeneration and Angiogenesis

Layer-by-Layer Microneedle-Mediated rhEGF Transdermal Delivery for Enhanced Wound Epidermal Regeneration and Angiogenesis

Evidence for Natural Products as Alternative Wound-Healing Therapies

New Smart Bioactive and Biomimetic Chitosan-Based Hydrogels for Wounds Care Management

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