Therapeutic Application of Pineapple Protease (Bromelain): A Review

Tochi, Bitange Nipa; Wang, Zhang; Xu, Shuangyan; Zhang, Wenbin

doi:10.3923/pjn.2008.513.520

Cited by 103 publications

(34 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many of those naturally found PIs were further characterized from different plant species which mainly included trypsin from serine protease group which have been tested for various diseases (Richardson, 1991; Tamir et al, 1996; Majumdar, 2013). This review explains about PIs of all earlier reported plant species that have been used as therapeutic agents and tested against different diseases and human disorders (Table 1; Murugesan et al, 2001; Neuhof et al, 2003; Troncoso et al, 2003; Kobayashi et al, 2004; Lanza et al, 2004; Clemente et al, 2005, 2012; Kim et al, 2005; Suzuki et al, 2005; Capaldi et al, 2007; Banerjee et al, 2008; Tochi et al, 2008; Caccialupi et al, 2010; Hsieh et al, 2010; Joanitti et al, 2010; García-Gasca et al, 2012; Magee et al, 2012; de Paula et al, 2012a; Borodin et al, 2013; Ferreira et al, 2013; Rakashanda et al, 2013b; Clemente and Arques, 2014; Souza et al, 2014) and future scope to search for new species, which are as follows:…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

“…Similar folding and disulfide bond connectivity were found to be shared in Bromelain inhibitor VI from pineapple stem (Stem bromelain, BI-VI) with the Bowman-Birk trypsin/chymotrypsin inhibitor from soybean (BBI-I) (Hatano et al, 1996). It is essentially a group of sulfhydryl proteolytic enzymes that includes a variety of cysteine proteases (Tochi et al, 2008). Along with protease inhibitors, it also encompasses peroxidases, acid phosphatase, organically bound calcium and remains stable over a wide range of pH 2–9 (Hatano et al, 1996; Tochi et al, 2008).…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

“…It is essentially a group of sulfhydryl proteolytic enzymes that includes a variety of cysteine proteases (Tochi et al, 2008). Along with protease inhibitors, it also encompasses peroxidases, acid phosphatase, organically bound calcium and remains stable over a wide range of pH 2–9 (Hatano et al, 1996; Tochi et al, 2008). Even though, both stem bromelain and fruit bromelain are single-chain glycosylated enzymes, stem bromelain has low proteolytic ability and specificity for peptide bonds compared to fruit bromelain.…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

“…Proteolytic activity of bromelain has been shown to play a minor part in pharmacological activity while other factors of it, such as immune-modulatory, hormone-like properties, fibrinolytic activity and uncharacterized components such as CCS and CCZ compliments also contributes toward its pharmacological activity (Tochi et al, 2008). However, it is necessary to investigate further on these uncharacterized components.…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

See 3 more Smart Citations

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

2016

View full text Add to dashboard Cite

Plants are known to have many secondary metabolites and phytochemical compounds which are highly explored at biochemical and molecular genetics level and exploited enormously in the human health care sector. However, there are other less explored small molecular weight proteins, which inhibit proteases/proteinases. Plants are good sources of protease inhibitors (PIs) which protect them against diseases, insects, pests, and herbivores. In the past, proteinaceous PIs were considered primarily as protein-degrading enzymes. Nevertheless, this view has significantly changed and PIs are now treated as very important signaling molecules in many biological activities such as inflammation, apoptosis, blood clotting and hormone processing. In recent years, PIs have been examined extensively as therapeutic agents, primarily to deal with various human cancers. Interestingly, many plant-based PIs are also found to be effective against cardiovascular diseases, osteoporosis, inflammatory diseases and neurological disorders. Several plant PIs are under further evaluation in in vitro clinical trials. Among all types of PIs, Bowman-Birk inhibitors (BBI) have been studied extensively in the treatment of many diseases, especially in the field of cancer prevention. So far, crops such as beans, potatoes, barley, squash, millet, wheat, buckwheat, groundnut, chickpea, pigeonpea, corn, and pineapple have been identified as good sources of PIs. The PI content of such foods has a significant influence on human health disorders, particularly in the regions where people mostly depend on these kind of foods. These natural PIs vary in concentration, protease specificity, heat stability, and sometimes several PIs may be present in the same species or tissue. However, it is important to carry out individual studies to identify the potential effects of each PI on human health. PIs in plants make them incredible sources to determine novel PIs with specific pharmacological and therapeutic effects due to their peculiarity and superabundance.

show abstract

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

See 2 more Smart Citations

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

2016

View full text Add to dashboard Cite

show abstract

“…Bromelain contains peroxidase, acid phosphatase, several protease inhibitors and organically bound calcium and have anti-inflammatory properties as it blocks the formation of kinins which are responsible for inflammations [8]. Bromelain is used for relieving the pains, sprains, swelling, bruises, and arthritis and assist in diluting mucous and thus is beneficial for treating sinusitis, bronchitis, sore throat and tuberculosis [9].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical, Proximate and Sensory Properties of Pineapple (<i>Ananas</i> sp.) Syrup Developed from Its Organic Side-Stream

Tortoe¹,

Johnson²,

Slaghek³

et al. 2013

FNS

View full text Add to dashboard Cite

A major economical industrial challenge from pineapple (Ananas sp.) processing contributing to environmental pollution is the organic side-streams of pineapple. The physicochemical, proximate and sensory properties of organic sidestream pineapple syrup (OSPS) developed from Smooth cayenne, Sugar loaf and MD 2 pineapple varieties were evaluated. Organic side-stream pineapple syrup developed from MD 2 recorded the highest moisture content with a corresponding water activity. The colour change in OSPS was significant among the three varieties and Sugar loaf variety deviated from the standard yellow colour more than Smooth cayenne and MD 2 varieties. This was buttress by the high Total Soluble Solids in 10% and 20% dilutions of Sugar loaf. The OSPS was acidic. In bread, incorporating 5% OSPS (w:w) of Sugar loaf recorded the highest percentage acceptability among the pineapple varieties. Interestingly, in the production of cakes with 15%, 20% and 30% OSPS, MD 2 recorded the highest percentage overall acceptance. For bread and cake, there was varied significance (P < 0.05) of the sensory properties for appearance, colour, aroma, taste, texture, crust, mouthfeel and overall acceptance. Notably, cake and bread with incorporated OSPS of 15% MD 2 and 5% Sugar loaf was more acceptable.

show abstract

Stability and Bioaccessibility of Fruit Bioactives in Foods: Food Component Interactions and Matrix Effect

Sun‐Waterhouse¹

2013

Bioactives in Fruit

View full text Add to dashboard Cite

Therapeutic Application of Pineapple Protease (Bromelain): A Review

Cited by 103 publications

References 36 publications

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

Physicochemical, Proximate and Sensory Properties of Pineapple (<i>Ananas</i> sp.) Syrup Developed from Its Organic Side-Stream

Stability and Bioaccessibility of Fruit Bioactives in Foods: Food Component Interactions and Matrix Effect

Contact Info

Product

Resources

About

Therapeutic Application of Pineapple Protease (Bromelain): A Review

Cited by 103 publications

References 36 publications

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

Physicochemical, Proximate and Sensory Properties of Pineapple (&lt;i&gt;Ananas&lt;/i&gt; sp.) Syrup Developed from Its Organic Side-Stream

Stability and Bioaccessibility of Fruit Bioactives in Foods: Food Component Interactions and Matrix Effect

Contact Info

Product

Resources

About

Physicochemical, Proximate and Sensory Properties of Pineapple (<i>Ananas</i> sp.) Syrup Developed from Its Organic Side-Stream