Therapeutic Angiogenesis of Bone Marrow Mononuclear Cells (MNCs) and Peripheral Blood MNCs: Transplantation for Ischemic Hindlimb

Zhang, Hongkun; Zhang, Nan; Li, Ming; Huang, Feng; Jin, Wei�; Zhao, Haige; Chen, Xudong; Tian, Lu

doi:10.1016/j.avsg.2007.07.037

Cited by 37 publications

(22 citation statements)

References 27 publications

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“…We have also shown that transplantation of BMMCs promoted angiogenesis after ischemic stroke as well as after other ischemic diseases such as limb ischemia [16,17] and myocardial infarction [18,19]. In this study, we intravenously transplanted BMMCs and found that most of GFP-positive BMMCs accumulated in and around poststroke region in brains.…”

Section: Discussionmentioning

confidence: 55%

“…Cell transplantation using bone marrow mononuclear cells (BMMCs) has been well-documented to accelerate angiogenesis/neovascularization in the several ischemic diseases such as limb ischemia [16,17] and myocardial infarction [18,19]. BMMCs contain endothelial progenitor cells (EPCs) [20] that have been shown to contribute to revascularization of ischemic tissues and repair of injured endothelium [21].…”

Section: Introductionmentioning

confidence: 99%

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Bone Marrow Mononuclear Cells Promote Proliferation of Endogenous Neural Stem Cells Through Vascular Niches After Cerebral Infarction

et al. 2010

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Increasing evidence shows that administration of bone marrow mononuclear cells (BMMCs) is a potential treatment for various ischemic diseases, such as ischemic stroke. Although angiogenesis has been considered primarily responsible for the effect of BMMCs, their direct contribution to endothelial cells (ECs) by being a functional elements of vascular niches for neural stem/progenitor cells (NSPCs) has not been considered. Herein, we examine whether BMMCs affected the properties of ECs and NSPCs, and whether they promoted neurogenesis and functional recovery after stroke. We compared i.v. transplantations 1 3 10 6 BMMCs and phosphate-buffered saline in mice 2 days after cortical infarction. Systemically administered BMMCs preferentially accumulated at the postischemic cortex and periinfarct area in brains; cell proliferation of ECs (angiogenesis) at these regions was significantly increased in BMMCstreated mice compared with controls. We also found that endogenous NSPCs developed in close proximity to ECs in and around the poststroke cortex and that ECs were essential for proliferation of these ischemia-induced NSPCs. Furthermore, BMMCs enhanced proliferation of NSPCs as well as ECs. Proliferation of NSPCs was suppressed by additional treatment with endostatin (known to inhibit proliferation of ECs) following BMMCs transplantation. Subsequently, neurogenesis and functional recovery were also promoted in BMMCs-treated mice compared with controls. These results suggest that BMMCs can contribute to the proliferation of endogenous ischemia-induced NSPCs through vascular niche regulation, which includes regulation of endothelial proliferation. In addition, these results suggest that BMMCs transplantation has potential as a novel therapeutic option in stroke treatment.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Bone Marrow Mononuclear Cells Promote Proliferation of Endogenous Neural Stem Cells Through Vascular Niches After Cerebral Infarction

et al. 2010

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“…All patients received PBMNCs intramuscularly. After two and 12 months, ABI, TcPO 2 , claudication-free walking distance and pain intensity improved significantly (p<0.005) [23]. A significant increase in new vessel formation was also evidenced by angiography at 24 weeks.…”

Section: Stem Cells From Peripheral Blood 421 Pbmncsmentioning

confidence: 79%

“…Thus, while both treatment options were effective, PBMNC administration yielded higher overall efficacy [20]. Finally, Zhang et al [23] studied 15 patients (10 with PAD, 5 with TAO) suffering from severe ischaemia affecting the popliteal and distal arterial segment who could not be surgically revascularised. Four patients were Fontaine stage II, five patients were stage III, and six patients were stage IV.…”

Section: Stem Cells From Peripheral Blood 421 Pbmncsmentioning

confidence: 99%