Therapeutic agents from endophytes harbored in Asian medicinal plants

Sharma, Ayushi; Malhotra, Bhanu; Kharkwal, Harsha; Kulkarni, Giriraj T.; Kaushik, Nutan

doi:10.1007/s11101-020-09683-8

Cited by 26 publications

(18 citation statements)

References 148 publications

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“…For a comprehensive review of the use of endophytes as therapeutic agents in Asian medicinal plants see the recent paper by Sharma and colleagues (Sharma et al . 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Culturable diversity of bacterial endophytes associated with medicinal plants of the Western Ghats, India

Webster

Mullins

Cunningham-Oakes

et al. 2020

FEMS Microbiology Ecology

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Bacterial endophytes are found in the internal tissues of plants and have intimate associations with their host. However, little is known about the diversity of medicinal plant endophytes (ME) or their capability to produce specialised metabolites that may contribute to therapeutic properties. We isolated 75 bacterial ME from 24 plant species of the Western Ghats, India. Molecular identification by 16S rRNA gene sequencing grouped MEs into 13 bacterial genera, with members of Gammaproteobacteria and Firmicutes being the most abundant. To improve taxonomic identification, 26 selected ME were genome sequenced and average nucleotide identity (ANI) used to identify them to the species-level. This identified multiple species in the most common genus as Bacillus. Similarly, identity of the Enterobacterales was also distinguished within Enterobacter and Serratia by ANI and core-gene analysis. AntiSMASH identified non-ribosomal peptide synthase, lantipeptide and bacteriocin biosynthetic gene clusters (BGC) as the most common BGCs found in the ME genomes. Five of the ME isolates belonging to Bacillus, Serratia and Enterobacter showed antimicrobial activity against the plant pathogen Pectobacterium carotovorum. Using molecular and genomic approaches we have characterised a unique collection of endophytic bacteria from medicinal plants. Their genomes encode multiple specialised metabolite gene clusters and the collection can now be screened for novel bioactive and medicinal metabolites.

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“…For a comprehensive review of the use of endophytes as therapeutic agents in Asian medicinal plants see the recent paper by Sharma and colleagues (Sharma et al . 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“… 2015 ; Sharma et al . 2020 ) and their investigation offers huge potential in identifying new pharmaceutical compounds.…”

Section: Introductionmentioning

confidence: 99%

Culturable diversity of bacterial endophytes associated with medicinal plants of the Western Ghats, India

Webster

Mullins

Cunningham-Oakes

et al. 2020

FEMS Microbiology Ecology

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“…drived from brown alga gave a novel chlorinated bezophenone compounds with perceivable antibiotic activity against MRSA as well as from a marine red alga T. islandicus , hydroanthraquinones with antioxidative and antimicrobial properties was isolated [ 11 , 38 , 39 ]. Clavicipitaceous endophytes have applied potential as biocontrol agents against insect pests of grasses, hence exploiting these naturally occurring symbioses, isolating their mycobiota to use and develope their natural products as biocides may prevent the requirement for chemical pesticides in managed grasslands [ 40 , 41 ]. The clavicipitaceous fungi of concern within the Clavicipitaceae owned by a relatively small class of species have common characteristics in being endophytically connected with grasses as obligate biotrophic symbionts [ 42 , 43 ].…”

Section: Main Textmentioning

confidence: 99%

“…The variation in the colonization of fungal endophytes can described by the fact that symbiotic fungi can benefit plants by alleviating abiotic plants by pressures associated with climate change as heat and drought as well as maintaining stable biological communities, especially despite environmental concerns [ 27 , 53 , 54 ]. It is obvious that biodiversity is a valuable source of innovation not only in ascertaining a plethora of up till now undefined species and their evolutionary backgrounds, genetics, ecology, and the abundance of implicit precious molecules [ 40 ]. It is a revolution of supposed an exhausted observation favorable to change sights of reductionist investigation of the past decades into snapshots of a dynamic world of systems biology [ 54 ] through elaborating combinatorial methods (microbial, chemical, and biochemical) that can lead to the discovery of bioactive agents that can be further improved based on their pharmaceutical, medicinal or other biotechnology potential [ 3 , 4 , 5 , 6 , 7 , 13 , 15 , 30 , 55 ].…”

Section: Main Textmentioning

confidence: 99%

Marine endophytic fungal metabolites: A whole new world of pharmaceutical therapy exploration

El-Bondkly

2021

Heliyon

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The growing threat arises due to diseases such as cancer and the infections around the world leading to a critical requirement for novel and constructive compounds with unique ways of action capable of combating these deadly diseases. At present, it is evident that endophytic fungi constitute an enormous as well as comparatively untapped source of great biodiversity that can be considered as a wellspring of effective novel natural products for medical, agricultural and industrial use. Marine endophytic fungi have been found in every marine plants (algae, seagrass, driftwood, mangrove plants), marine vertebrates (mainly, fish) or marine invertebrates (mainly, sponge and coral) inter- and intra-cellular without causing any palpable symptoms of illness. Since evolution of microbes and eukaryotes to a higher level, coevolution has resulted in specific interaction mechanisms. Endophytic fungi are known to influence the life cycle and are necessary for the homeostasis of their eukaryotic hosts and the chemical signals of their host have been shown to activate gene expression in endophytes to induce expression of endophytic secondary metabolites. Marine endophytic fungi are receiving increasing attention by chemists because of their varied and structurally unmatched compounds that have strong biological roles in life as lead pharmaceutical compounds, including anticancer, antiviral, insulin mimetic, antineurodegenerative, antimicrobial, antioxidant and immuno-suppressant compounds. Moreover, fungal endophytes proved to have different biological activities for exploitation in the environmental and agricultural sustainability.

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“…Endophytes are microorganisms that inhabit the tissues of living plants and possess a resourceful reservoir of secondary metabolites with therapeutic potential [18]. Endophytic bacteria are a good source of biomaterials for the synthesis of AgNPs that could be utilized as effective antimicrobial agents [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacteria Enterobacter hormaechei Fabricated Silver Nanoparticles and Their Antimicrobial Activity

Monowar

Rahman²,

Bhore

et al. 2021

Pharmaceutics

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Antimicrobial resistance (AMR), one of the greatest issues for humankind, draws special attention to the scientists formulating new drugs to prevent it. Great emphasis on the biological synthesis of silver nanoparticles (AgNPs) for utilization in single or combinatorial therapy will open up new avenues to the discovery of new antimicrobial drugs. The purpose of this study was to synthesize AgNPs following a green approach by using an endophytic bacterial strain, Enterobacter hormaechei, and to assess their antimicrobial potential against five pathogenic and four multidrug-resistant (MDR) microbes. UV-Vis spectroscopy, fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and zeta potential (ζ) were used to characterize the synthesized AgNPs. Endophytic E. hormaechei-mediated AgNPs (Eh-AgNPs) were represented by a strong UV-Vis absorbance peak at 418 nm within 5 min, forming spherical and polydispersed nanoparticles in the size range of 9.91 nm to 92.54 nm. The Eh-AgNPs were moderately stable with a mean ζ value of −19.73 ± 3.94 mV. The presence of amine, amide, and hydroxyl functional groups was observed from FTIR analysis. In comparison to conventional antibiotics, the Eh-AgNPs were more effective against Bacillus cereus (ATCC 10876) and Candida albicans (ATCC 10231), exhibiting 9.14 ± 0.05 mm and 8.24 ± 0.05 mm zones of inhibition (ZOIs), respectively, while displaying effective inhibitory activity with ZOIs ranging from 10.98 ± 0.08 to 13.20 ± 0.07 mm against the MDR bacteria. Eh-AgNP synthesis was rapid and eco-friendly. The results showed that Eh-AgNPs are promising antimicrobial agents that can be used in the development and formulation of new drugs to curb the menace of antimicrobial resistance in pathogenic and MDR microbes.

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Therapeutic agents from endophytes harbored in Asian medicinal plants

Cited by 26 publications

References 148 publications

Culturable diversity of bacterial endophytes associated with medicinal plants of the Western Ghats, India

Culturable diversity of bacterial endophytes associated with medicinal plants of the Western Ghats, India

Marine endophytic fungal metabolites: A whole new world of pharmaceutical therapy exploration

Endophytic Bacteria Enterobacter hormaechei Fabricated Silver Nanoparticles and Their Antimicrobial Activity

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