Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots

Muthu, Madaswamy S; Kulkarni, Sneha A.; Raju, Anandhkumar; Feng, Si‐Shen

doi:10.1016/j.biomaterials.2012.01.036

Cited by 232 publications

(117 citation statements)

References 32 publications

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“…181 Muthu et al prepared tocopheryl polyethylene glycol 1000 succinate-coated theranostic liposomes containing Dox and quantum dots with and without targeting moieties. 182 Folic acid was used as the targeting probe to target folate receptors overexpressing MCF-7 breast cancer cell lines. Along similar lines, Wen et al developed quantum dots and apomorphineincorporated theranostic liposomes to eliminate uptake by the liver and to enhance brain targeting.…”

Section: Liposomes In Theranosticsmentioning

confidence: 99%

Liposomes as nanomedical devices

Bozzuto¹,

Molinari²

2015

IJN

1,669

1,080

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Since their discovery in the 1960s, liposomes have been studied in depth, and they continue to constitute a field of intense research. Liposomes are valued for their biological and technological advantages, and are considered to be the most successful drug-carrier system known to date. Notable progress has been made, and several biomedical applications of liposomes are either in clinical trials, are about to be put on the market, or have already been approved for public use. In this review, we briefly analyze how the efficacy of liposomes depends on the nature of their components and their size, surface charge, and lipidic organization. Moreover, we discuss the influence of the physicochemical properties of liposomes on their interaction with cells, half-life, ability to enter tissues, and final fate in vivo. Finally, we describe some strategies developed to overcome limitations of the “first-generation” liposomes, and liposome-based drugs on the market and in clinical trials.

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Section: Liposomes In Theranosticsmentioning

confidence: 99%

Liposomes as nanomedical devices

Bozzuto¹,

Molinari²

2015

IJN

1,669

1,080

View full text Add to dashboard Cite

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“…TPGS can also greatly improve the performance of TPGS-emulsified nanoparticles, leading to enhanced cellular uptake and in vitro cancer cell mortality, and more desirable in vivo pharmacokinetics. 22,23 In addition, TPGS has been reported to efficiently inhibit the growth of human lung cancer cells both in vivo and in vitro. 24 Furthermore, TPGS produces synergistic growth inhibition effects upon combination with other anticancer drugs due to its ability to induce apoptosis in tumor cells.…”

Section: Abbad Et Almentioning

confidence: 99%

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Abbad¹,

Wang²,

Waddad³

et al. 2015

IJN

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Herein, we describe the preparation of a targeted cellular delivery system for morin hydrate (MH), based on a low-molecular-weight hyaluronic acid-poly(butyl cyanoacrylate) (HA-PBCA) block copolymer. In order to enhance the therapeutic effect of MH, D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) was mixed with HA-PBCA during the preparation process. The MH-loaded HA-PBCA “plain” nanoparticle (MH-PNs) and HA-PBCA/TPGS “mixed” nanoparticles (MH-MNs) were concomitantly characterized in terms of loading efficiency, particle size, zeta potential, critical aggregation concentration, and morphology. The obtained MH-PNs and MH-MNs exhibited a spherical morphology with a negative zeta potential and a particle size less than 200 nm, favorable for drug targeting. Remarkably, the addition of TPGS resulted in about 1.6-fold increase in drug-loading. The in vitro cell viability experiment revealed that MH-MNs enhanced the cytotoxicity of MH in A549 cells compared with MH solution and MH-PNs. Furthermore, blank MNs containing TPGS exhibited selective cytotoxic effects against cancer cells without diminishing the viability of normal cells. In addition, the cellular uptake study indicated that MNs resulted in 2.28-fold higher cellular uptake than that of PNs, in A549 cells. The CD44 receptor competitive inhibition and the internalization pathway studies suggested that the internalization mechanism of the nanoparticles was mediated mainly by the CD44 receptors through a clathrin-dependent endocytic pathway. More importantly, MH-MNs exhibited a higher in vivo antitumor potency and induced more tumor cell apoptosis than did MH-PNs, following intravenous administration to S180 tumor-bearing mice. Overall, the results imply that the developed nanoparticles are promising vehicles for the targeted delivery of lipophilic anticancer drugs.

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“…Os complexos obtidos foram caracterizados em termos de tamanho, polidispersidade e potencial zeta conforme apresentado na Tabela 3. É possível perceber que em Ca = 0,009, 0,001 e 0,005 há formação de duas populações de nanopartículas analisando tamanho em intensidade e/ou número e polidispersidade acima de 0,25; o que poderia dificultar a transfecção já que estudos in vitro demonstraram que nanopartículas de tamanho entre 100 e 200 nm e polidispersidade menor que 0,2 (distribuição de tamanho homogênea) são mais facilmente internalizadas por células animais in vitro, supondo que elas despenderiam menos energia na captura (Muthu et al, 2012;Rejman et al, 2004). Já em Ca = 0,0008 e 0,003 há somente uma população de nanopartículas homogênea com polidispersidade abaixo de 0,2, porém o potencial zeta das nanopartículas ficou menor em Ca = 0,003 demostrando que houve melhor incorporação de pDNA (carga negativa) aos lipossomas catiônicos.…”

Section: Investigação Do Número De Capilaridade (Ca) Com W F = 033 Eunclassified

Sistemas Microfluídicos De Gotas Para Incorporação De Dna Em Lipossomas Catiônicos Para Aplicação Em Terapia Gênica

Vitor¹,

Lima²,

Torre³

2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A terapia gênica visa à inserção de ácidos nucleicos em células-alvo, porém uma melhor eficiência de transfecção se dá com menor tamanho e polidispersidade dos complexos. Sistemas microfluídicos baseados em gotas são capazes de promover uma mistura rápida, gerando complexos com estas características. Neste contexto, utilizou-se no sistema microfluídico de gotas o óleo biocompatível FC-40 como fase contínua e a solução aquosa de lipossoma catiônico juntamente com pDNA, como fase dispersa. Incorporou-se pDNA nos lipossomas fixando o número de capilaridade (Ca), número adimensional que relaciona as forças viscosas e a tensão superficial entre as duas fases imiscíveis, em 0,003. Os lipossomas após incorporação do pDNA continuaram com o tamanho de aproximadamente 80 nm, a polidispersidade aumentou de 0,18 para 0,20 e potencial zeta diminuiu de 60 para 45 mV. Assim, este sistema nos permitiu obter complexos com propriedades desejáveis para transfecção. INTRODUÇÃOA terapia gênica refere-se à inserção de ácidos nucleicos em células-alvo ou em órgãos com consequente expressão do material genético modificado (Guang Liu e De Yao, 2002). Um dos fatores importantes para o sucesso da terapia gênica é o desenvolvimento de sistemas capazes de transferir o gene de interesse de forma eficiente para o interior das células em um processo denominado transfecção (Verma e Weitzman, 2005). Esses vetores podem ser virais ou não-virais, e dentre estes últimos, lipossomas catiônicos destacam-se nesta função (Miller, 1998).Lipossomas catiônicos são sistemas auto-agregados formados por lipídios de natureza anfifílica que geram bicamadas, as quais na presença de água em excesso formam vesículas esféricas. Lipídios catiônicos garantem a carga positiva dos lipossomas, podendo assim interagir eletrostaticamente com ácidos nucleicos, carregados negativamente, obtendo-se complexos capazes de serem incorporados nas células (Miller, 1998). No entanto, a obtenção de complexos lipossoma catiônico/ácido nucléico requer reprodutibilidade das propriedades físico-químicas para garantir eficiência na aplicação biológica.

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Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots

Cited by 232 publications

References 32 publications

Liposomes as nanomedical devices

Liposomes as nanomedical devices

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Sistemas Microfluídicos De Gotas Para Incorporação De Dna Em Lipossomas Catiônicos Para Aplicação Em Terapia Gênica

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