Theory of frontal analysis and displacement development

Binding mechanism of nine N-phenylpiperazine derivatives and α1A-adrenoceptor using site-directed molecular docking and high performance affinity chromatography

“…The binding mechanism of the N-phenylpiperazine derivatives to a 1A -AR was next analyzed via affinity chromatography using frontal analysis. 33 The capacity factors of each compound at 20 C in the initial mobile phase (without addition of any compounds) were calculated using k 0 ¼ (t R À t 0 )/t 0 , where k 0 is the capacity factor, t R is the retention time of the injection compound, and t 0 is the void time of the chromatographic system determined using NaNO 2 which is an un-retained solute on the column. The mobile phase was Tris-buffer (5.0 mM, pH 7.2) containing 1.0 mM NaCl and 0.5 mM EDTA.…”

Chromatography in the Gas‐solid System

“…Further progress was realized when Tiselius (1943) introduced a highly adsorbed substance as a displacing agent. These early studies culminated in the mastering of displacement chromatography from both the theoretical (Claeson, 1946(Claeson, , 1949 and methodological points of view (Turner, 1943;Phillips, 1949Phillips, , 1952Clough, 1955). In the period 1950 to 1952 this type of gas chromatography was superseded by the more advantageous elution method.…”

“…The frontal type of chromatographic analysis (Roth et al, 1942;Claeson, 1946Claeson, , 1949 was an outgrowth of the earlier work, but it achieved only limited importance. Schuftan (1930Schuftan ( , 1931 introduced the use of a gas as a desorption agent.…”

History of Liquid Chromatography

“…A técnica se aplica muito bem para análises preparativas, qualitativas e quantitativas, sendo os resultados limitados pela dificuldade de se encontrar o agente de deslocamento ideal em separações de misturas desconhecidas. 42 A técnica desenvolvida por Tiselius e Claesson foi aplicada com muito sucesso na separação de aminoácidos em colunas de troca iônica com eluição por deslocamento promovido pela variação de pH da fase móvel. Este tema será retomado mais a frente com a técnica utilizada e melhorada por Moore e Stein.…”