Theory of Catalytic Hydrogen Waves in Organic Polarography

Mairanovskii, S. G.

doi:10.1070/rc1964v033n01abeh001373

Cited by 20 publications

(12 citation statements)

References 3 publications

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“…The result of bulk electrolysis showed that no significant change in both peak current and peak potential was observed before and after electrolysis even it takes 5 hours. These results supported that deduction on the observed reduction wave as a catalytic adsorptive one [19,32,33].…”

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confidence: 89%

Voltammetric Behavior of Telmisartan and Cathodic Adsorptive Stripping Voltammetric Method for Its Assay in Pharmaceutical Dosage Forms and Biological Fluids

et al. 2010

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Electrochemical behavior of Telmisartan and optimum conditions for its assay were investigated by using cyclic voltammetry and square-wave voltammetry. All studies were based on the quasi-reversible and adsorption-controlled electrochemical reduction signal of TS at about À1.50 V versus Ag/AgCl at pH 10.0 in Britton-Robinson buffer. The peak current was found to change linearly with concentration from 1.69 nM (0.87 mg/L) to 27.5 nM (14.15 mg/ L). The limit of detection and the limit of quantification were found to be 1.05 nM (0.54 mg/L) and 3.49 nM (1.79 mg/L), respectively. The method was successfully applied to different samples with good recoveries at about 100 %.

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confidence: 89%

Voltammetric Behavior of Telmisartan and Cathodic Adsorptive Stripping Voltammetric Method for Its Assay in Pharmaceutical Dosage Forms and Biological Fluids

et al. 2010

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“…Native and denatured BSA in 0.1 M KCl and at acid pH values Scheller et al 20 used for their measurements (unbuffered) 0.1 M KCl, pH 7 or pH 3 as background electrolytes. These media are not the best for signals due to the catalytic hydrogen evolution (known to increase with the buffer capacity); 23,24 we used them, however, to compare our data with those of Scheller et al 20 In 0.1 M KCl, pH 7 we did not obtain the CPS peak H but only poorly developed inflections with 100 nM BSA in both native and denatured states (Fig. 1a).…”

Section: Resultsmentioning

confidence: 87%

Potential-dependent surface denaturation of BSA in acid media

Paleček

Ostatná

2009

Analyst

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In contrast to previous reports claiming bovine serum albumin (BSA) denaturation at mercury surfaces, recently it has been shown that BSA and other proteins do not denature as a result of adsorption to the mercury electrodes at alkaline and neutral pH values. In this pH range, constant current chronopotentiometry (CPS) with mercury or solid amalgam electrodes can be used to distinguish between native, denatured and damaged BSA. Here we show that at acid pH values (around pH 4.5) native and denatured BSA yield almost the same CPS responses suggesting denaturation of native BSA at the electrode surface. Under these conditions BSA is, however, not denatured at the electrode at accumulation potentials (E(A) values) close to the potential of zero charge, but at E(A) values more negative than -0.8 V, after destabilization of the surface-attached BSA by electroreduction of some disulfide groups at about -0.48 V and by electric field effects at more negative potentials.

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“…The CHER theory in low molecular weight organic compounds and proteins was developed more than 50 years ago,56 at a time when protein chemistry was not able to provide detailed structural information of the studied proteins. Moreover, techniques for preparation of highly purified wild‐type and mutant proteins were not available.…”

Section: New Trends In Label‐free Protein Electrocatalysismentioning

confidence: 99%

Electrocatalysis in proteins, nucleic acids and carbohydrates

Paleček

Bartošík

Ostatná

et al. 2012

The Chemical Record

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The ability of proteins to catalyze hydrogen evolution has been known for more than 80 years, but the poorly developed d.c. polarographic "pre-sodium wave" was of little analytical use. Recently, we have shown that by using constant current chronopotentiometric stripping analysis, proteins produce a well-developed peak H at hanging mercury drop and solid amalgam electrodes. Peak H sensitively reflects changes in protein structures due to protein denaturation, single amino acid exchange, etc. at the picomole level. Unmodified DNA and RNA do not yield such a peak, but they produce electrocatalytic voltammetric signals after modification with osmium tetroxide complexes with nitrogen ligands [Os(VIII)L], binding covalently to pyrimidine bases in nucleic acids. Recently, it has been shown that six-valent [Os(VI)L] complexes bind to 1,2-diols in polysaccharides and oligosaccharides, producing voltammetric responses similar to those of DNA-Os(VIII)L adducts. Electrocatalytic peaks produced by Os-modified nucleic acids, proteins (reaction with tryptophan residues) and carbohydrates are due to the catalytic hydrogen evolution, allowing determination of oligomers at the picomolar level.

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Theory of Catalytic Hydrogen Waves in Organic Polarography

Cited by 20 publications

References 3 publications

Voltammetric Behavior of Telmisartan and Cathodic Adsorptive Stripping Voltammetric Method for Its Assay in Pharmaceutical Dosage Forms and Biological Fluids

Voltammetric Behavior of Telmisartan and Cathodic Adsorptive Stripping Voltammetric Method for Its Assay in Pharmaceutical Dosage Forms and Biological Fluids

Potential-dependent surface denaturation of BSA in acid media

Electrocatalysis in proteins, nucleic acids and carbohydrates

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