Theoretical understanding of the SPR sensor response on the protein adsorption

Abstract

This research deals with consideration of the SPR sensor response in the framework of the scattering matrix approach with the modeling of biomolecular layer using Green's function formalism and effective medium theories. It was found out that modeling of the SPR sensor response using abovementioned approaches in the densely packed monolayer approximation gave not enough agreement with the experiment. The reason was that real molecular layers are rarely densely packed. They usually can be characterized by molecular surface concentration or monolayer filling factor. Approximation of these parameters allows obtaining their values, which correspond to real biomolecular layers, and gives better agreement with the experiment. ExperimentalCommercially available optoelectronic SPR biosensor NanoSPR-321 was used in the present

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

“…SPR‐based sensors are highly sensitive to changes in the refractive index near the metallic film. The application of an external potential difference to the gold film might lead to its oxidation , or modify the charges distribution in the double layer at the metal‐liquid interface . This could result in an SPR angular shift, which would depend on the voltage and the frequency applied.…”