Theoretical studies on the interactions of XIAP-BIR3 domain with bicyclic and tricyclic core monovalent Smac mimetics

Ling, Baoping; Dong, Lihua; Zhang, Rui; Wang, Zhiguo; Liu, Yongjun; Liu, Chengbu

doi:10.1016/j.jmgm.2010.09.011

Cited by 4 publications

(1 citation statement)

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“…All of IAPs family proteins contained one to three characteristic BIR domains, and also contained RING-finger domain. The BIR and RING-finger domains play important roles against apoptosis [20][21][22]. IAPs could inhibit apoptosis by combining and interacting between the BIR motifs and other apoptotic-related proteins [23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of an inhibitor of apoptosis protein (IAPs) in freshwater pearl mussel, Hyriopsis schlegelii

Wang

Zhang

et al. 2019

Bioengineered

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The inhibitor of apoptosis proteins (IAPs) played important roles in inhibiting the apoptosis of tumor cells by regulating caspase activity in mammals. In this study, we first cloned the full-length cDNA sequence of IAPs gene (designated as Hs-IAPs) in Hyriopsis schlegelii. The Hs-IAPs gene contained an open reading frame of 1719 nucleotides, encoding a predicted protein of 572 amino acids. qRT-PCR assay indicated that the Hs-IAPs gene was ubiquitously expressed in different tissues, and the highest expression level was in gills. Furthermore, we purified and obtained the recombinant protein of Hs-IAPs which showed a molecular weight of 82.5 kDa. We used H 2 O 2 stimulation experiment to explore the possible function of Hs-IAPs. The results showed that the percentage of viable cells significantly increased following the Hs-IAPs concentration. These indicated that the Hs-IAPs may play a role in antioxidation causing by H 2 O 2 , and its anti-oxidative may be crucial in the process of apoptosis. H 2 O 2 stimulation analysis indicated that the Hs-IAPs plays a role in inhibiting apoptosis.

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