Theoretical Investigation of the Bond Dissociation of Hydroxyl Terminated Polybutadiene Binder and Effect on Mechanical Properties

Wu, Wenming; Zhang, Wei; Chen, Min-B Qiang Hong-Fu; Shi, Liang-Wei

doi:10.6023/a1202061

Cited by 8 publications

(6 citation statements)

References 14 publications

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“…Liver organoids, have also exhibited an improved activity of CYP3A4, a key mediator of drug clearance. [ 149 ] The inclusion of inflammatory cytokines such as TNF‐α were found to support the expansion of the organoids in vitro, with hepatocytes retaining their functionality and engraftment potential. These organoids were found to regenerate mice (FAH−/− mice) liver tissues and respond to Wnt signals, known to promote cellular proliferation, both during homeostasis and regeneration (Figure 4B).…”

Section: Decm Hydrogels For Engineering Organoids/assembloids For Tis...mentioning

confidence: 99%

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Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Moura

Monteiro

Ferreira

et al. 2022

Adv Funct Materials

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The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next‐generation tissue‐specific hydrogels with close‐to‐native biomolecular signatures and user‐tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue‐specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom‐up cell engineering technologies may unravel unprecedented tissue‐like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient‐tailored living therapeutics.

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Section: Decm Hydrogels For Engineering Organoids/assembloids For Tis...mentioning

confidence: 99%

“…Reproduced under the terms of the Creative Commons CC‐BY‐NC‐ND license. [ 149 ] Copyright 2019, European Association for the Study of the Liver. B) Widefield image of liver section stained for FAH and TBX3.…”

Section: Decm Hydrogels For Engineering Organoids/assembloids For Tis...mentioning

confidence: 99%

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Moura

Monteiro

Ferreira

et al. 2022

Adv Funct Materials

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“…[ 166 ] Thus, several protocols have been achieved to generate different types of liver organoids, such as hepatic organoids, [ 167 ] cholangiocyte organoids, [ 168,169 ] hepato‐biliary‐pancreatic organoids, [ 170 ] and hepato‐biliary organoids. [ 171 ]…”

Section: Modeling Organoid Systems By Applying Microfluidic Techniquesmentioning

confidence: 99%

Microfluidic Techniques for Next‐Generation Organoid Systems

Gong

Kang

et al. 2022

Adv Materials Inter

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PSCs (iPSCs) and embryonic stem cells (ESCs), or adult stem cells (ASCs). Pioneered in the Yoshiki Sasai lab, where cortical tissues and optic-cuplike structures were firstly induced from ESCs in vitro, [1,2] organoid technology is regarded as a milestone in the stem cell field because it cast light upon the path to stably growing self-aggregating and selfrenewing stem cells into native tissues by mimicking developmental processes. [3] Subsequently, the rapidly developing field of organoid technology is advancing the maturity of technology for developing 3D macrostructures of organs such as the retina, [4] heart, [5] liver, [6] kidney, [7] and intestines. [8] In marked contrast to traditional 2D monocultures of cell lines losing cell-cell and cell-matrix interactions, organoids maintain and define in situ phenotypes fairly well. Despite organoids having shown great progress in tissue morphogenesis and organogenesis, the lack of organ-specific biochemical and physical microenvironments and nonphysiological shape and size have limited the function and real-life applications of organoids. Therefore, innovative strategies are urgently needed to precisely control perfusion conditions, nutrient supply, and mechanical cues in order to establish an optimal microenvironment for organoid cultures.The recently developed organs-on-a-chip (OOC) technology provides an in vivo-like microenvironment, showing great potential to enhance our understanding of organ development, physiology, and pathology. [9] Besides, the application of dynamic flow conditions or mechanical stimuli provided by OOCs can improve the terminal maturation of cells. [10,11] OOCs usually are comprised of specialized cell types and tissue elements (biochemical and biophysical cues of in vivo microenvironment), aiming to capture key functions of a specific organ. [12] Organoids rely on the self-organization of growing stem cell aggregates, whereas OOC is based on a reductionist engineering approach. In addition, organoids are multicellular 3D tissue with an architecture resembling some aspects of original tissues, which are not classified as OOC because of their production through stochastic self-organization from PSCs or ASCs and lack of microenvironment elements. [13] However, organoid and OOC techniques each have their own advantages, and they can be appropriately combined to enable highfidelity human modeling from stem cells. [14] Such synergistic Organoids are 3D multicellular structures derived from pluripotent stem cells (PSCs) or adult stem cells (ASCs), which have attracted increasing interest in the fields of drug screening, cell therapy, and regenerative medicine. Despite considerable success in culturing organoids with native microanatomy, challenges to achieving a physiologically relevant microenvironment remain. Complex dynamic feedback between cells and the extracellular matrix and uncontrollable mechano-physiological cues hamper the further study of organoid systems. Innovative engineering approaches are needed to produce, control, and analy...

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“…[ 333 ] Moreover, PSC/iPSC‐derived 3D liver organoids can be generated by starting either with a coculture of cell‐types, for instance, iPSC‐derived endodermal, endothelial, and mesenchymal cells, [ 346 ] or with a homogeneous cell population that during the culture protocol differentiates into the different hepatic cell‐lineages. [ 347,348 ] The use of PSCs/iPSCs allows to generate multicellular 3D models with all hepatic cell lineages incorporated being genetically identical. [ 336,347 ] Human iPSCs derived from fibroblasts, blood cells or any other cell‐type of a donor, can be differentiated in all hepatic cell‐types and thus present an unlimited pool of cellular material for diagnostic purposes or toxicity testing.…”

Section: Human Organ Structure and Functions And Their Modeling In Vitromentioning

confidence: 99%

Stem Cells for Next Level Toxicity Testing in the 21st Century

Fritsche

Haarmann‐Stemmann

Kapr

et al. 2020

Small

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The call for a paradigm change in toxicology from the United States National Research Council in 2007 initiates awareness for the invention and use of human‐relevant alternative methods for toxicological hazard assessment. Simple 2D in vitro systems may serve as first screening tools, however, recent developments infer the need for more complex, multicellular organotypic models, which are superior in mimicking the complexity of human organs. In this review article most critical organs for toxicity assessment, i.e., skin, brain, thyroid system, lung, heart, liver, kidney, and intestine are discussed with regards to their functions in health and disease. Embracing the manifold modes‐of‐action how xenobiotic compounds can interfere with physiological organ functions and cause toxicity, the need for translation of such multifaceted organ features into the dish seems obvious. Currently used in vitro methods for toxicological applications and ongoing developments not yet arrived in toxicity testing are discussed, especially highlighting the potential of models based on embryonic stem cells and induced pluripotent stem cells of human origin. Finally, the application of innovative technologies like organs‐on‐a‐chip and genome editing point toward a toxicological paradigm change moves into action.

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Theoretical Investigation of the Bond Dissociation of Hydroxyl Terminated Polybutadiene Binder and Effect on Mechanical Properties

Cited by 8 publications

References 14 publications

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Microfluidic Techniques for Next‐Generation Organoid Systems

Stem Cells for Next Level Toxicity Testing in the 21st Century

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