CO 2 underground storage is an important approach to reduce carbon emissions; meanwhile, the combination of CO 2 storage and economic benefits can further promote the long-term development of carbon neutrality. Global tight oil reserves are abundant, but the depletion development of most tight reservoirs is unsatisfactory. Therefore, it is crucial to utilize CO 2 storage combined with enhanced oil recovery (EOR) for tight reservoir development. At present, there are few experimental studies on the CO 2 effective distance and CO 2 -EOR storage of huff-n-puff in tight reservoirs. In this paper, the CO 2 effective distance and the evolution of saturation profiles in tight cores are first investigated, and CO 2 -EOR storage is quantitatively evaluated for CO 2 huff-npuff in tight reservoirs based on CT and nuclear magnetic resonance technology. Meanwhile, the influences of oil composition and permeability on the effective distance and CO 2 -EOR storage are further investigated. The results indicate that the increase of CO 2 effective distance gradually slows down with time, and the decrease of permeability significantly reduces the effective distance. Increasing amount of oil is mainly produced in larger pores, which is more than twice of that in smaller pores. CO 2 -EOR storage experiments quantify that the CO 2 storage ratio for a 0.22 × 10 −3 μm2 core is up to 72.31% under different depressurization conditions (12, 8, and 0.1 MPa). With the decrease in production pressure, oil recovery gradually increases, while the CO 2 storage ratio decreases, indicating that there is a collaborative optimization to maximize the oil production and CO 2 storage ratio. Comparison experiments reveal that the oil composition (replaced by fluorocarbon oil) has little influence on the effective distance and CO 2 -EOR storage, while the influence of permeability is significant. The CO 2 effective distance determines the sweep efficiency, so increasing effective distance is essential to enhance oil recovery and CO 2 storage. This research is of great significance for CO 2 utilization and CO 2 storage in tight reservoirs.