Theoretical investigation of nitric oxide interaction with aluminum phthalocyanine

Silva, Valter H. C.; Martins, Marcos Paulo de Oliveira; Oliveira, Heibbe C. B. de; Camargo, Ademir J.

doi:10.1016/j.jmgm.2010.10.008

Cited by 8 publications

(5 citation statements)

References 74 publications

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“…To account for the extremely rapid rise in NO levels during AlClPc photo−activation, we hypothesize that NO is buffered by this phthalocyanine photosensitizer and released from the cell exposed to laser irradiation. This mechanism is supported by quantum chemistry computations, showing that the metal center of AlClPc binds NO in a redox-dependent manner [ 58 ]. Indeed, there is ample evidence that phthalocyanines can be specifically modified to act as highly efficient NO reservoirs and photosensitive NO donors that also produce singlet oxygen [ 59 , 60 ].…”

Section: Discussionmentioning

confidence: 88%

Critical role of gap junction communication, calcium and nitric oxide signaling in bystander responses to focal photodynamic injury

et al. 2015

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Ionizing and nonionizing radiation affect not only directly targeted cells but also surrounding “bystander” cells. The underlying mechanisms and therapeutic role of bystander responses remain incompletely defined. Here we show that photosentizer activation in a single cell triggers apoptosis in bystander cancer cells, which are electrically coupled by gap junction channels and support the propagation of a Ca2+ wave initiated in the irradiated cell. The latter also acts as source of nitric oxide (NO) that diffuses to bystander cells, in which NO levels are further increased by a mechanism compatible with Ca2+-dependent enzymatic production. We detected similar signals in tumors grown in dorsal skinfold chambers applied to live mice. Pharmacological blockade of connexin channels significantly reduced the extent of apoptosis in bystander cells, consistent with a critical role played by intercellular communication, Ca2+ and NO in the bystander effects triggered by photodynamic therapy.

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Section: Discussionmentioning

confidence: 88%

Critical role of gap junction communication, calcium and nitric oxide signaling in bystander responses to focal photodynamic injury

et al. 2015

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“…[19][20][21] Here we show that ROS/ RNS production by AlClPc photoactivation accelerates or abolishes Ca 2C oscillations in the widely used insulin-secreting MIN6 b-cell line. To guide the interpretation of our results, we used a mathematical model of Ca 2C handling adapted to MIN6 cells.…”

Section: Introductionmentioning

confidence: 94%

Reactive oxygen and nitrogen species disturb Ca²⁺oscillations in insulin-secreting MIN6 β-cells

Antonucci

Tagliavini

Pedersen

2015

Islets

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Keywords: calcium pumps, mathematical modeling, pulsatile insulin secretion, photosensitizer, ROS/RNS Abbreviations: AlClPc, Aluminum Phthalocyanine Chloride; CICR, calcium-induced calcium release; CuFL, selective turn-on fluorescent NO reporter formed by a Cu(II) complex of a fluorescein modified with an appended metal-chelating ligand (FL); ER, endoplasmic reticulum; IP 3 R, inositol triphospate receptor; MitoSOX, mitochondrial superoxide indicator; NO, nitric oxide; PMCA, plasma membrane Ca 2C ATPase; RNS, reactive nitrogen species; ROS, reactive oxygen species; RyR, ryanodine receptor; SERCA, Sarcoplasmic/endoplasmic reticulum Ca 2C ATPase; TEA, tetraethylammonium; Tg, thapsigargin.Disturbances in pulsatile insulin secretion and Ca 2C oscillations in pancreatic b-cells are early markers of diabetes, but the underlying mechanisms are still incompletely understood. Reactive oxygen/nitrogen species (ROS/RNS) are implicated in reduced b-cell function, and ROS/RNS target several Ca 2C pumps and channels. Thus, we hypothesized that ROS/RNS could disturb Ca 2C oscillations and downstream insulin pulsatility. We show that ROS/RNS production by photoactivation of aluminum phthalocyanine chloride (AlClPc) abolish or accelerate Ca 2C oscillations in the MIN6 b-cell line, depending on the amount of ROS/RNS. Application of the sarcoplasmic/endoplasmic reticulum Ca 2C ATPase (SERCA) inhibitor thapsigargin modifies the Ca 2C response to high concentrations of ROS/RNS. Further, thapsigargin produces effects that resemble those elicited by moderate ROS/RNS production. These results indicate that ROS/RNS interfere with endoplasmic reticulum Ca 2C handling. This idea is supported by theoretical studies using a mathematical model of Ca 2C handling adapted to MIN6 cells. Our results suggest a putative link between ROS/RNS and disturbed pulsatile insulin secretion. IntroductionInsulin is secreted from the pancreatic b-cells in distinct pulses 1,2 and disturbed pulsatile insulin release has been suggested to be an early marker of diabetes. 3 These bursts of insulin are driven by underlying oscillations in electrical activity and cytosolic Ca 2C levels, which periodically trigger Ca 2C -regulated exocytosis. 4 Importantly, secretory pulses are more effective than constant insulin levels in lowering plasma glucose levels. 3,5,6 In particular, hepatic signaling is highly sensitive to whether insulin is released in pulses, 7 leading to the suggestion that insulin resistance in the liver is secondary to disturbed pulsatile insulin release. 8 Thus, a deeper understanding of oscillatory b-cell activity and its disturbance has clinical relevance.Disturbed insulin secretion and diabetes have been associated with b-cell damage caused by reactive oxygen species (ROS). 9 However, ROS have also been shown to exert a stimulating short-term effect on insulin release. 10 Hence, ROS may act as a double-edged sword: moderate and short-term elevations play a positive signaling role augmenting insulin secretion, but prolonged exposure to elevated ROS...

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“…In an attempt to explain the influence of the central metal on the interaction between MPc or MPor and NO molecules, the authors of other works turned to quantum chemical calculations [ 68 , 69 , 72 ]. For example, Nguyen with co-authors [ 68 , 69 ] studied the adsorption of nitric oxide (NO) on various MPcs with M = Mn, Fe, Co, Ni, Cu, Zn using DFT calculations.…”

Section: Sensing Layers For Detecting No In Aqueous Mediamentioning

confidence: 99%

Recent Advances in Phthalocyanine and Porphyrin-Based Materials as Active Layers for Nitric Oxide Chemical Sensors

Klyamer

Shutilov

Basova

2022

Sensors

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Nitric oxide (NO) is a highly reactive toxic gas that forms as an intermediate compound during the oxidation of ammonia and is used for the manufacture of hydroxylamine in the chemical industry. Moreover, NO is a signaling molecule in many physiological and pathological processes in mammals, as well as a biomarker indicating the course of inflammatory processes in the respiratory tract. For this reason, the detection of NO both in the gas phase and in the aqueous media is an important task. This review analyzes the state of research over the past ten years in the field of applications of phthalocyanines, porphyrins and their hybrid materials as active layers of chemical sensors for the detection of NO, with a primary focus on chemiresistive and electrochemical ones. The first part of the review is devoted to the study of phthalocyanines and porphyrins, as well as their hybrids for the NO detection in aqueous solutions and biological media. The second part presents an analysis of works describing the latest achievements in the field of studied materials as active layers of sensors for the determination of gaseous NO. It is expected that this review will further increase the interest of researchers who are engaged in the current level of evaluation and selection of modern materials for use in the chemical sensing of nitric oxide.

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Theoretical investigation of nitric oxide interaction with aluminum phthalocyanine

Cited by 8 publications

References 74 publications

Critical role of gap junction communication, calcium and nitric oxide signaling in bystander responses to focal photodynamic injury

Critical role of gap junction communication, calcium and nitric oxide signaling in bystander responses to focal photodynamic injury

Reactive oxygen and nitrogen species disturb Ca²⁺oscillations in insulin-secreting MIN6 β-cells

Recent Advances in Phthalocyanine and Porphyrin-Based Materials as Active Layers for Nitric Oxide Chemical Sensors

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Theoretical investigation of nitric oxide interaction with aluminum phthalocyanine

Cited by 8 publications

References 74 publications

Critical role of gap junction communication, calcium and nitric oxide signaling in bystander responses to focal photodynamic injury

Critical role of gap junction communication, calcium and nitric oxide signaling in bystander responses to focal photodynamic injury

Reactive oxygen and nitrogen species disturb Ca2+oscillations in insulin-secreting MIN6 β-cells

Recent Advances in Phthalocyanine and Porphyrin-Based Materials as Active Layers for Nitric Oxide Chemical Sensors

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Reactive oxygen and nitrogen species disturb Ca²⁺oscillations in insulin-secreting MIN6 β-cells