In this paper, we present a two-dimensional numerical analysis of the conjugate natural convection and radiation heat transfer in a double-space enclosure with two semitransparent walls. Two kinds of boundary conditions are considered, the first being the isothermal process of the opaque wall, and the other the incidence of a constant radiation flux in the left semitransparent wall. The renormalization group k-ε model is adopted to simulate the turbulent flow in the enclosure. To compute the radiation heat transfer in a semitransparent medium, the discrete ordinates model is used. We compare the behaviors of enclosures with single and double semitransparent walls and determine the difference in the results obtained for semitransparent and opaque partitions. The results indicate that a semitransparent partition facilitates a reduction in the heat loss or obtains a higher temperature distribution. The transmittance of a semitransparent wall has a great effect on the thermal and flow characteristics in an enclosure. The change of wall temperature is found to be significant when the thermal conductivity values range from 0.05 to 0.5 W/(m•K), and to be small when ranging from 0.5 to 10 W/(m•K). These conclusions are helpful for green design and energy saving in solar buildings.