Theoretical insights into the reaction mechanism and kinetics of ampicillin degradation with hydroxyl radical

Aydogdu, Seyda; Hatipoğlu, Arzu

doi:10.1007/s00894-023-05462-2

Cited by 6 publications

(2 citation statements)

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“…Concerns regarding the potential contamination of water resources by various emerging organic contaminants are growing globally (EOCs) [ 1 ]. Pharmaceuticals are a fundamental group of EOCs, including antibiotics, antidiabetics, antiepileptics, antimalarial, analgesics, and anti-inflammatories, which are frequently reported in freshwater and marine environments [ 2 , 3 , 4 ]. Many of these drugs are not biodegraded during wastewater treatment and are discharged to aquatic environments in an active form [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of the Synergistic Toxicity of Ampicillin and Cefazoline on Selenastrum capricornutum

Huang,

Qin,

et al. 2024

Toxics

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Ampicillin (AMP) and cefazolin (CZO) are commonly used β-lactam antibiotics which are extensively globally produced. Additionally, AMP and CZO are known to have relatively high ecotoxicity. Notably, the mix of AMP and CZO creates a synergistic effect that is more harmful to the environment, and how exposure to AMP-CZO can induce synergism in algae remains virtually unknown. To yield comprehensive mechanistic insights into chemical toxicity, including dose–response relationships and variations in species sensitivity, the integration of multiple endpoints with de novo transcriptomics analyses were used in this study. We employed Selenastrum capricornutum to investigate its toxicological responses to AMP and CZO at various biological levels, with the aim of elucidating the underlying mechanisms. Our assessment of multiple endpoints revealed a significant growth inhibition in response to AMP at the relevant concentrations. This inhibition was associated with increased levels of reactive oxygen species (ROS) and perturbations in nitrogen metabolism, carbohydrate metabolism, and energy metabolism. Growth inhibition in the presence of CZO and the AMP-CZO combination was linked to reduced viability levels, elevated ROS production, decreased total soluble protein content, inhibited photosynthesis, and disruptions in the key signaling pathways related to starch and sucrose metabolism, ribosome function, amino acid biosynthesis, and the production of secondary metabolites. It was concluded from the physiological level that the synergistic effect of Chlorophyll a (Chla) and Superoxide dismutase (SOD) activity strengthened the growth inhibition of S. capricornutum in the AMP-CZO synergistic group. According to the results of transcriptomic analysis, the simultaneous down-regulation of LHCA4, LHCA1, LHCA5, and sodA destroyed the functions of the photosynthetic system and the antioxidant system, respectively. Such information is invaluable for environmental risk assessments. The results provided critical knowledge for a better understanding of the potential ecological impacts of these antibiotics on non-target organisms.

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Section: Introductionmentioning

confidence: 99%

“…Few studies have examined the toxicity of mixtures like the ones mentioned above [25]. The AMP acute toxicity is calculated as 160 mg•L −1 , 172 mg•L −1 , and 1530 mg•L −1 at 96 h for green algae, daphnid, and fish, respectively [2]. The EC 50 of cefazolin to S. capricornutum is calculated as > 1000 mg•L −1 [34].…”

Section: Introductionmentioning

confidence: 99%