Theoretical considerations underlying Na+ uptake mechanisms in freshwater fishes

Parks, Scott K.; Tresguerres, Martín; Goss, Greg G.

doi:10.1016/j.cbpc.2008.03.002

Cited by 80 publications

(91 citation statements)

References 93 publications

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“…A similar mechanism has been shown in crustacean gill (Onken and Riestenpatt, 1998) and in fish gill (Reid et al, 2003). Another proposed mechanism for Na + uptake involves the Na + /H + exchangers; however, it has been suggested that this mechanism would only be feasible under brackish or saline water conditions as the low Na + concentration in freshwater would not be sufficient for Na + /H + exchange (see Parks et al, 2008). In mosquito whole-larval flux studies, Na + uptake was linked to H + secretion (Stobbart, 1971).…”

Section: Na + Uptake Mechanism In Anal Papillaementioning

confidence: 59%

Pharmacological characterisation of apical Na+ and Cl– transport mechanisms of the anal papillae in the larval mosquitoAedes aegypti

Duca

Nasirian

Galperin

et al. 2011

Journal of Experimental Biology

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SUMMARYThe anal papillae of freshwater mosquito larvae are important sites of NaCl uptake, thereby acting to offset the dilution of the hemolymph by the dilute habitat. The ion-transport mechanisms in the anal papillae are not well understood. In this study, the scanning ion-selective electrode technique (SIET) was utilized to measure ion fluxes at the anal papillae, and pharmacological inhibitors of ion transport were utilized to identify ion-transport mechanisms. Na + uptake by the anal papillae was inhibited by bafilomycin and phenamil but not by HMA. Cl -uptake was inhibited by methazolamide, SITS and DIDS but not by bafilomycin. H

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Section: Na + Uptake Mechanism In Anal Papillaementioning

confidence: 59%

Pharmacological characterisation of apical Na+ and Cl– transport mechanisms of the anal papillae in the larval mosquitoAedes aegypti

Duca

Nasirian

Galperin

et al. 2011

Journal of Experimental Biology

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“…Interestingly, Perry and colleagues (Ivanis et al, 2008) recently used double ISH and/or ICC to colocalize the mRNA and protein of both NHE2 and -3 in PNA + MR cells in gill sections of rainbow trout. This evidence appears to be against the argument of Goss's group, as described above, of the unfavourable operation of NHE in isolated trout gill cells (Parks et al, 2007;Parks et al, 2008). These differences between zebrafish and rainbow trout indicate that apical Na + -uptake mechanisms may be species specific.…”

Section: H + -Atpase Rich (Hr) Cellsmentioning

confidence: 79%

“…PNA -and PNA + MR cells, which were isolated with PNA, express higher H + -ATPase and NKA, respectively (Galvez et al, 2002), and show different ion-transport functions: PNA -exhibited a bafilomycin- sensitive acid-activated 22 Na + -uptake/acid-stimulated response of H + -ATPase, while PNA + showed Ca 2+ uptake (Reid et al, 2003;Galvez et al, 2006;Parks et al, 2007). Based on many previous immunocytochemical, physiological and pharmacological data, the current model for trout gill cells proposes that PNA -and PNA + MR cells are responsible for Na + uptake/acid secretion and Ca 2+ uptake/base secretion/Cl -uptake, respectively (Tresguerres et al, 2006;Perry and Gilmour, 2006;Evans, 2008;Parks et al, 2008). In this situation, PNA -and PNA + MR cells in rainbow trout gills appear to be analogues of zebrafish HR and NaR cells, respectively; however, things are not so simple.…”

Section: Comparison Of Ionocytes Between Speciesmentioning

confidence: 99%

“…In a recent review, Parks and colleagues (Parks et al, 2008) emphasized the thermodynamic constraints that prevent electroneutral apical NHE from functioning in most FW environments, thus favouring the applicability of ENaC-like channels to fish Na + -uptake mechanisms. Interestingly, Perry and colleagues (Ivanis et al, 2008) recently used double ISH and/or ICC to colocalize the mRNA and protein of both NHE2 and -3 in PNA + MR cells in gill sections of rainbow trout.…”

Section: H + -Atpase Rich (Hr) Cellsmentioning

confidence: 99%

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Ion uptake and acid secretion in zebrafish (Danio rerio)

Hwang

2009

Journal of Experimental Biology

161

162

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SummaryTransepithelial transport is one of the major processes involved in the mechanism of homeostasis of body fluids in vertebrates including fish. The current models of ion regulation in fish gill ionocytes have been proposed mainly based on studies in traditional model species like salmon, trout, tilapia, eel and killifish, but the mechanisms are still being debated due to the lack of convincing molecular physiological evidence. Taking advantage of plentiful genetic databases for zebrafish, we studied the molecular/cellular mechanisms of ion regulation in fish skin/gills. In our recently proposed model, there are at least three subtypes of ionocytes in zebrafish skin/gills: Na + -K + -ATPase-rich (NaR), Na + -Cl -cotransporter (NCC) and H + -ATPase-rich (HR) cells. Specific isoforms of transporters and enzymes have been identified as being expressed by these ionocytes: zECaC, zPMCA2 and zNCX1b by NaR cells; zNCC gill form by NCC cells; and zH + -ATPase, zNHE3b, zCA2-like a and zCA15a by HR cells. Serial molecular physiological experiments demonstrated the distinct roles of these ionocytes in the transport of various ions: HR, NaR and NCC cells are respectively responsible for acid secretion/Na + uptake, Ca 2+ uptake and Cl -uptake. The expression, regulation and function of transporters in HR and NaR cells are much better understood than those in NCC cells. The basolateral transport pathways in HR and NCC cells are still unclear, and the driving forces for the operations of apical NHE and NCC are another unresolved issue. Studies on zebrafish skin/gill ionocytes are providing new insights into fish ion-regulatory mechanisms, but the zebrafish model cannot simply be applied to other species because of species differences and a lack of sufficient molecular physiological evidence in other species.

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“…The gills are probably the organs that consume the most energy during osmoregulation (Sangiao-Alvarellos et al, 2003). More specifically, the gill Na + , K + ATPase, located mainly in chloride cells, requires energy input and plays an important role in osmoregulation in both hyperosmotic (Jobling, 1995;Evans et al, 2005) and hyposmotic (Evans, 2008;Parks et al, 2008) environments. The activity of this ATPase spends at least half of the energy consumed during Na + /H + exchange in ionocytes (Kirschner, 2004).…”

Section: Introductionmentioning

confidence: 99%

The effect of salinity on osmoregulation and development of the juvenile fat snook, Centropomus parallelus (POEY)

et al. 2013

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Eurihaline fish support waters with different salt concentration. However, numerous studies have shown that salinity can affect fish development. Thus, the effect of salinity change from 20 to 5 and 35 on survival, weight, length, gill chloride cell ultrastructure and gill Na + , K + ATPase activity was evaluated in Centropomus parallelus following short-term (6, 24 and 96 hours) and long-term exposure (30 and 60 days). Salinity did not affect C. parallelus survival, final weight and length. The quantity of chloride cells increased visibly at salinities of 5 and 35, with the cells exhibiting the typical features of uptake and secretory cells, respectively. Na + , K + ATPase activity in the gill of the C. parallelus was significantly greater at a salinity of 5 than at a salinity of 20 or 35 after 96 hours, but not after 30 or 60 days. These results indicate that salinity change from high to low salt water induces gill chloride cell and Na + , K + ATPase activity adaptations after short-term exposure. However, after long-term exposure at salinity 5, gill Na + , K + ATPase activity is no more necessary at high levels. The increase in salinity to 35 does not induce significant change in gills. Juveniles of C. parallelus may thus be capable of acclimating to salinities of 5 to 35 for 60 days without significant effects on development.Keywords: Centropomus parallelus, chloride cell, osmoregulation, Na + , K + ATPase. O efeito da salinidade sobre a osmoregulação e o desenvolvimento do juvenil de robalo-peva, Centropomus parallelus (POEY) ResumoPeixes eurihalinos suportam águas com diferentes concentrações de sal. Contudo, muitos estudos têm mostrado que a salinidade pode afetar o desenvolvimento do peixe. Portanto, o efeito da mudança de salinidade de 20 para 5 e 35 na taxa de sobrevivência, peso, comprimento, morfologia das células de cloreto branquiais e atividade da Na + , K + ATPase foram avaliadas no Centropomus parallelus após curto (6, 24 e 96 horas) e longo tempo de exposição (30 e 60 dias). A salinidade não afetou a sobrevivência, o peso e comprimento final do robalo-peva. A quantidade de células de cloreto aumentou visivelmente nas salinidades 5 e 35, exibindo morfologias típicas de células que absorvem e secretam sal, respectivamente. A atividade da Na + , K + ATPase nas brânquias do C. parallelus foi significativamente maior na salinidade 5 do que nas salinidades 20 ou 35 após 96 horas, mas não após 30 e 60 dias. Esses resultados indicam que a mudança de alta para baixa salinidade provoca adaptações nas células de cloreto e na atividade da Na + , K + ATPase branquial em curto prazo. Contudo, após longa exposição na salinidade 5, a alta atividade da Na + , K + ATPase branquial não é mais necessária. O aumento de salinidade para 35 não induz mudanças significativas nas brânquias. Portanto, juvenis de C. parallelus possuem a capacidade de aclimatação nas salinidades de 5 a 35 sem efeitos significativos no desenvolvimento após 60 dias.Palavras-chave: Centropomus parallelus, célula de cloreto, osmoregula...

show abstract

Theoretical considerations underlying Na+ uptake mechanisms in freshwater fishes

Cited by 80 publications

References 93 publications

Pharmacological characterisation of apical Na+ and Cl– transport mechanisms of the anal papillae in the larval mosquitoAedes aegypti

Pharmacological characterisation of apical Na+ and Cl– transport mechanisms of the anal papillae in the larval mosquitoAedes aegypti

Ion uptake and acid secretion in zebrafish (Danio rerio)

The effect of salinity on osmoregulation and development of the juvenile fat snook, Centropomus parallelus (POEY)

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