Theoretical considerations regarding the cell membrane function during cold-induced preservation of tissues and organs: New possibilities for optimizing the process

Dragomir, C.T.; Păuşescu, E

doi:10.1016/0022-5193(74)90198-2

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Glucose-stimulated insulin release during the subsequent incubation was only 20-25% of that of fresh islets. Although basal insulin release had been achieved, it is possible that the low concentration of glucose during the preincubation had not allowed proper restoration of the intra-fi-cell milieu, since intracellular metabolism had probably been altered during the cold storage (16). Therefore, a 4-h period with 18 mM glucose at 370C was added between the storage and the preincubation in an attempt to allow the islets to recover.…”

Section: Discussionmentioning

confidence: 99%

Maintenance of insulin release from pancreatic islets stored in the cold for up to 5 weeks.

Frankel¹,

Gylfe²,

Hellman³

et al. 1976

J. Clin. Invest.

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A B S T R A C T Insulin content and release were measured from hand-dissected pancreatic islets from noninbred ob/ob mice after 1-5 wk storage in tissue culture medium 199 at various temperatures and glucose concentrations.After storage of islets for 1 wk at 37°, 22°, or 80C in 18 mM glucose medium and preincubation with 1 mM glucose, glucose-stimulated insulin release during the subsequent incubation was only 20-35% of that of fresh islets. The addition of a 4-h period at 370C with 18 mM glucose between the cold storage and perincubation restored glucose-stimulated insulin release from 80C-stored islets to fresh-islet levels. Release throughout the 1-18 mM glucose range was strikingly parallel to that of fresh islets. Exposure of fresh islets to the same 4-h period increased basal release but did not affect maximal release.When islets were stored at 80C with 18 mM glucose for more than 1 wk, a short period at 370C every week was necessary for maintenance of release. After 5 wk of this procedure, glucose-stimulated insulin release was one-third that of fresh islets, or similar to that of islets stored for only 1 wk at 370C. Storage at 80C for 1 wk with 3 mM glucose, or continuously for 3 or 5 wk with 18 mM glucose, maintained islet insulin content, whereas release was lost.Thus, glucose-stimulated insulin release is best maintained by storage of pancreatic islets in tissue culture medium with a high concentration of glucose at 80C with short weekly periods at 370C.

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Section: Discussionmentioning

confidence: 99%

Maintenance of insulin release from pancreatic islets stored in the cold for up to 5 weeks.

Frankel¹,

Gylfe²,

Hellman³

et al. 1976

J. Clin. Invest.

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“…Specifically, these efforts cover important issues such as intracellular ice formation [29; 37], solute interactions [27], effects of temperature on cell membrane function [11], and synthetic phase diagrams [17]. While such studies are important in improving our fundamental understanding of these processes, little work has been done to investigate the methods of vitrification.…”

Section: Introductionmentioning

confidence: 99%

Mathematical modeling of cryoprotectant addition and removal for the cryopreservation of engineered or natural tissues

2012

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Long-term storage of natural tissues or tissue-engineered constructs is critical to allow off-the-shelf availability. Vitrification is a method of cryopreservation that eliminates ice formation, as ice may be detrimental to the function of natural or bioartificial tissues. In order to achieve the vitreous state, high concentrations of CPAs must be added and later removed. The high concentrations may be deleterious to cells as the CPAs are cytotoxic and single-step addition or removal will result in excessive osmotic excursions and cell death. A previously described mathematical model accounting for the mass transfer of CPAs through the sample matrix and cell membrane was expanded to incorporate heat transfer and CPA cytotoxicity. Simulations were performed for two systems, an encapsulated system of insulin-secreting cells and articular cartilage, each with different transport properties, geometry and size. Cytotoxicity and mass transfer are dependent on temperature, with a higher temperature allowing more rapid mass transfer but also causing increased cytotoxicity. The effects of temperature are exacerbated for articular cartilage, which has larger dimensions and slower mass transport through the matrix. Simulations indicate that addition and removal at 4°C is preferable to 25°C, as cell death is higher at 25°C due to increased cytotoxicity in spite of the faster mass transport. Additionally, the model indicates that less cytotoxic CPAs, especially at high temperature, would significantly improve the cryopreservation outcome. Overall, the mathematical model allows the design of addition and removal protocols that ensure CPA equilibration throughout the sample while still minimizing CPA exposure and maximizing cell survival.

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Exceptional performance in heart preservation with an amino acid—Containing perfusion fluid

et al. 1978

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This is a report of a study aimed at putting into practice a new theory for hypothermic preservation of viable organs. A perfusion fluid elaborated according to this theory was applied in preservation of the heart, and resulted in storage of the heart for up to 72 hours with preservation of its functions (rhythm, presystolic ventricular pressure, systolic ventricular pressure, cardiac work, coronary blood pressure, sensitivity to drugs) and its morphology. An important finding was that repeated heart storage for 24 hours alternating with functional testing for 5-7 hours could be performed without irreversible alterations of cardiac function and fine structure. Furthermore, during functional testing following storage the hearts consistently demonstrated improvement of function in time, suggesting that the preserved myocardial tissues were able to rapidly achieve metabolic reequilibration. The results of this study provide the possibility of developing a system for efficient ex vivo heart conditioning before transplantation.

show abstract

Theoretical considerations regarding the cell membrane function during cold-induced preservation of tissues and organs: New possibilities for optimizing the process

Cited by 3 publications

References 15 publications

Maintenance of insulin release from pancreatic islets stored in the cold for up to 5 weeks.

Maintenance of insulin release from pancreatic islets stored in the cold for up to 5 weeks.

Mathematical modeling of cryoprotectant addition and removal for the cryopreservation of engineered or natural tissues

Exceptional performance in heart preservation with an amino acid—Containing perfusion fluid

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