An experiment was conducted on a prestressed concrete (PC) composite girder bridge with steel truss webs to investigate its flexural performance. The mechanical characteristics and failure modes of a PC composite girder bridge with steel truss webs was clarified. Finite element (FE) analysis was carried out, and the influence of the girder height-to-span ratio and eccentric loading effect on the flexural performance of a composite beam bridge with a steel truss web was discussed. The method for calculating the cracking bending moment, the bending moment at the rebar yield stage, and the ultimate bending moment of a PC composite girder with steel truss webs was proposed. Key findings include that, in both the elastic and cracking elastic stages, the strain of the bottom and top conforms to the plane-section assumption. Throughout the loading process, there was no occurrence of joint failure or local buckling failure in the steel truss webs; the composite girder ultimately fails due to excessive deformation, indicating that the overall mechanical performance of the composite beam is good. The deflection and stress in the mid-span section decrease with an increasing height-to-span ratio, and there are significant impacts of eccentric loading on deflection and stress. Compared with the results of the FE analysis and test, the calculation methods of the cracking moment, reinforcement yield moment, and ultimate moment of PC composite girders with steel truss webs presented in this paper have a high accuracy.