Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes

Camarda, Massimo; Fisicaro, Giuseppe; Anzalone, Ruggero; Alberti, Alessandra; Via, F. La; Magna, Antonino La; Ballo, Andrea; Giustolisi, G.; Minafra, Luigi; Cammarata, Francesco Paolo; Bravatà, Valentina; Forte, Giusi Irma; Russo, G.; Gilardi, M.C.

doi:10.1186/1475-925x-13-71

Cited by 19 publications

(18 citation statements)

References 29 publications

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“…Simulation results have proven that particle pearl chains are aligned along the direction of the perpendicular electric field based on the Monte Carlo method. Moreover, the distribution of the particles is in substantial agreement with some scholastic simulations [5,17,18,23], and a convergent total energy curve has been fitted in order to demonstrate the consistency of the particle formations. While the particles formed chains and columns in a uniform electric field without illumination in high frequencies, the chain arrangements in the chip are uncontrollable.…”

Section: Discussionsupporting

confidence: 62%

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Modeling of cellular pearl chain formation using a double photoconductive layer biochip

Zhao

Yang³

2015

Journal of Electrostatics

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Section: Discussionsupporting

confidence: 62%

“…Meanwhile, the time interval between two consecutive displacement events could be estimated as [23].…”

Section: Simulation Of Cellular Pearl Chain Based On the Monte Carlo mentioning

confidence: 99%

Modeling of cellular pearl chain formation using a double photoconductive layer biochip

Zhao

Yang³

2015

Journal of Electrostatics

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“…1C) on the bioparticles known as the dielectrophoresis (DEP) force (F DEP ). DEP is extensively studied in the literature (48,49) and briefly is modeled using three main equations (SI Materials and Methods). In these equations, the Clausius-Mossotti (CM) factor shows the effective polarizability of a bioparticle, where its magnitude determines the strength of the polarization effect and its sign determines the direction of the particle motion, which changes at the particle's cross-over frequency.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional, inexpensive, and reusable nanoparticle-printed biochip for cell manipulation and diagnosis

Esfandyarpour

DiDonato

Yang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Isolation and characterization of rare cells and molecules from a heterogeneous population is of critical importance in diagnosis of common lethal diseases such as malaria, tuberculosis, HIV, and cancer. For the developing world, point-of-care (POC) diagnostics design must account for limited funds, modest public health infrastructure, and low power availability. To address these challenges, here we integrate microfluidics, electronics, and inkjet printing to build an ultra-low-cost, rapid, and miniaturized lab-ona-chip (LOC) platform. This platform can perform label-free and rapid single-cell capture, efficient cellular manipulation, rare-cell isolation, selective analytical separation of biological species, sorting, concentration, positioning, enumeration, and characterization. The miniaturized format allows for small sample and reagent volumes. By keeping the electronics separate from microfluidic chips, the former can be reused and device lifetime is extended. Perhaps most notably, the device manufacturing is significantly less expensive, timeconsuming, and complex than traditional LOC platforms, requiring only an inkjet printer rather than skilled personnel and clean-room facilities. Production only takes 20 min (vs. up to weeks) and $0.01-an unprecedented cost in clinical diagnostics. The platform works based on intrinsic physical characteristics of biomolecules (e.g., size and polarizability). We demonstrate biomedical applications and verify cell viability in our platform, whose multiplexing and integration of numerous steps and external analyses enhance its application in the clinic, including by nonspecialists. Through its massive cost reduction and usability we anticipate that our platform will enable greater access to diagnostic facilities in developed countries as well as POC diagnostics in resource-poor and developing countries.lab on a chip | point of care | diagnostics | nanoparticles | microfluidics

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“…During all DEP experiments, one or few capsules were placed within electrode array to prevent capsule‐to‐capsule electrical interaction (e.g. chain formation ). Various AC field frequencies with a sinusoidal waveform were applied using a function generator (33250A, Agilent).…”

Section: Methodsmentioning

confidence: 99%

Dielectrophoretic characterization and isolation of jellyfish stinging capsules

et al. 2017

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Jellyfish stinging capsules known as nematocysts are explosive, natural-injection systems with high potential as a natural drug-delivery system. These organelles consist of a capsule containing a highly folded thin needle-like tubule and a matrix highly concentrated with charged constituents that enable the tubule to fire and penetrate a target. For the purpose of using these nematocysts as drug delivery system it is first required to purify subpopulations from heterogeneous population of capsules and to investigate each subpopulation's distinct function and characteristics. Here, the nematocysts' dielectric properties were experimentally investigated using dielectrophoretic and electrorotational spectra with best fits derived from theoretical models. The dielectric characterization adds to our understanding of the nematocysts' structure and function and is necessary for the dielectrophoretic isolation and manipulation of populations. As expected, the effect of monovalent and divalent exchange cations resulted in higher inner conductivity for the NaCl treated capsules; this result stands in agreement with their relative higher osmotic pressure. In addition, an efficient dielectrophoretic isolation of different nematocyst subpopulations was demonstrated, paving the way to an understanding of nematocysts' functional diversity and the development of an efficient drug delivery platform.

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Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes

Cited by 19 publications

References 29 publications

Modeling of cellular pearl chain formation using a double photoconductive layer biochip

Modeling of cellular pearl chain formation using a double photoconductive layer biochip

Multifunctional, inexpensive, and reusable nanoparticle-printed biochip for cell manipulation and diagnosis

Dielectrophoretic characterization and isolation of jellyfish stinging capsules

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