In 1975, P.R. Chernoff used iterates of the Laplacian on R n to prove an L 2 version of the Denjoy-Carleman theorem which provides a sufficient condition for a smooth function on R n to be quasi-analytic. In this paper we prove an exact analogue of Chernoff's theorem for all rank one Riemannian symmetric spaces of noncompact type using iterates of the associated Laplace-Beltrami operators. Moreover, we also prove an analogue of Chernoff's theorem for the sphere which is a rank one compact symmetric space.