Then there were five: a reexamination of the ant genus Paratrechina (Hymenoptera, Formicidae)

LaPolla, John S.; Fisher, Brian L.

doi:10.3897/zookeys.422.7779

Cited by 17 publications

(17 citation statements)

References 13 publications

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“…Unlike the conflicts involving T. allaborans and T. pacificum , Paratrechina longicornis was a case of morphologically identical specimens being sorted into two separate mOTUs differing by 5% (average uncorrected p ‐distances). Bearing in mind that P. longicornis is a widespread cosmopolitan species (Fox et al., ; McGlynn, ) of disputed native range (LaPolla & Fisher, ; Wetterer, ), high genetic variability in Asia may imply multiple introductions or one introduction involving multiple haplotypes (Bergsten et al., ; Wild, ). This species appears morphologically uniform despite high underlying genetic variability in mitochondrial markers.…”

Section: Discussionmentioning

confidence: 99%

Sorting specimen‐rich invertebrate samples with cost‐effective NGS barcodes: Validating a reverse workflow for specimen processing

Wang

Srivathsan

Foo

et al. 2018

Molecular Ecology Resources

105

168

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Biologists frequently sort specimen-rich samples to species. This process is daunting when based on morphology, and disadvantageous if performed using molecular methods that destroy vouchers (e.g., metabarcoding). An alternative is barcoding every specimen in a bulk sample and then presorting the specimens using DNA barcodes, thus mitigating downstream morphological work on presorted units. Such a "reverse workflow" is too expensive using Sanger sequencing, but we here demonstrate that is feasible with an next-generation sequencing (NGS) barcoding pipeline that allows for cost-effective high-throughput generation of short specimen-specific barcodes (313 bp of COI; laboratory cost <$0.50 per specimen) through next-generation sequencing of tagged amplicons. We applied our approach to a large sample of tropical ants, obtaining barcodes for 3,290 of 4,032 specimens (82%). NGS barcodes and their corresponding specimens were then sorted into molecular operational taxonomic units (mOTUs) based on objective clustering and Automated Barcode Gap Discovery (ABGD). High diversity of 88-90 mOTUs (4% clustering) was found and morphologically validated based on preserved vouchers. The mOTUs were overwhelmingly in agreement with morphospecies (match ratio 0.95 at 4% clustering). Because of lack of coverage in existing barcode databases, only 18 could be accurately identified to named species, but our study yielded new barcodes for 48 species, including 28 that are potentially new to science. With its low cost and technical simplicity, the NGS barcoding pipeline can be implemented by a large range of laboratories. It accelerates invertebrate species discovery, facilitates downstream taxonomic work, helps with building comprehensive barcode databases and yields precise abundance information.

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Section: Discussionmentioning

confidence: 99%

Sorting specimen‐rich invertebrate samples with cost‐effective NGS barcodes: Validating a reverse workflow for specimen processing

Wang

Srivathsan

Foo

et al. 2018

Molecular Ecology Resources

105

168

View full text Add to dashboard Cite

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“…High heterozygosity of workers, close association with humans, and high adaptability in disturbed environments of this species may help explain to some extent how this ant spread rapidly around the world even prior to the 20 th century (Weber, 1939; Harris and Berry, 2005; Lester, 2005; Wetterer, 2008; Pearcy et al, 2011). While the precise native range of this ant has been a source of debate and remains uncertain, distribution records of P. longicornis and its closest relatives suggest either a Southeast Asian or African origin (Wetterer, 2008; LaPolla et al, 2010; LaPolla et al, 2013; LaPolla and Fisher, 2014). A comprehensive phylogeographic study of P. longicornis is needed to help identify more precisely where the species originated as well as its subsequent dispersal routes around the globe.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Wolbachia Infection Patterns in a Globally Distributed Invasive Ant

et al. 2019

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Understanding the phylogeographic history of an invasive species may facilitate reconstructing the history and routes of its invasion. The longhorn crazy ant, Paratrechina longicornis, is a ubiquitous agricultural and household pest throughout much of the tropics and subtropics, but little is known about the history of its spread. Here, we examine worldwide genetic variation in P. longicornis and its associated Wolbachia bacterial symbionts. Analyses of mtDNA sequences of 248 P. longicornis workers (one per colony) from 13 geographic regions reveal two highly diverged mtDNA clades that co-occur in most of the geographic regions. These two mtDNA clades are associated with different Wolbachia infection patterns, but are not congruent with patterns of nDNA (microsatellite) variation. Multilocus sequence typing reveals two distinct Wolbachia strains in P. longicornis, namely, wLonA and wLonF. The evolutionary histories of these two strains differ; wLonA appears to be primarily transmitted maternally, and patterns of mtDNA and nDNA variation and wLonA infection status are consistent with a relatively recent Wolbachia-induced selective sweep. In contrast, the observed patterns of mtDNA variation and wLonF infections suggest frequent horizontal transfer and losses of wLonF infections. The lack of nDNA structure among sampled geographic regions coupled with the finding that numerous mtDNA haplotypes are shared among regions implies that inadvertent long-distance movement through human commerce is common in P. longicornis and has shaped the genetic structure of this invasive ant worldwide.

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“…The longhorn crazy ant, Paratrechina longicornis (Latreille, 1802), is regarded as a signifi cant invasive species due to its ecological impacts (Wetterer, 2008). The native range of this invasive species and its invasion history, however, remain controversial (Wetterer, 2008;LaPolla & Fisher, 2014). A previous study reported that colonies of P. longicornis from Bangkok, Thailand display a remarkable genetic system, whereby workers are produced by sexual reproduction, whereas queens are clones of their mothers and males are clones of their fathers (Pearcy et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of novel microsatellite markers for a globally distributed invasive ant Paratrechina longicornis (Hymenoptera: Formicidae)

Tseng¹,

Darras²,

Lee³

et al. 2019

Eur. J. Entomol.

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Then there were five: a reexamination of the ant genus Paratrechina (Hymenoptera, Formicidae)

Cited by 17 publications

References 13 publications

Sorting specimen‐rich invertebrate samples with cost‐effective NGS barcodes: Validating a reverse workflow for specimen processing

Sorting specimen‐rich invertebrate samples with cost‐effective NGS barcodes: Validating a reverse workflow for specimen processing

Genetic Diversity and Wolbachia Infection Patterns in a Globally Distributed Invasive Ant

Isolation and characterization of novel microsatellite markers for a globally distributed invasive ant Paratrechina longicornis (Hymenoptera: Formicidae)

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