Theileria

and, Dirk Dobbelaere; Baumgärtner, Martin

doi:10.1002/9783527629176.ch34

Cited by 14 publications

(12 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the schizont is strictly intracellular [13] and its presence crucial for the constitutive activation of the signaling pathways that drive proliferation and protection against apoptosis (reviewed in [6],[8]), we posit that this process is essential, not only for parasite persistence but also for the exponential expansion of the parasite population.…”

Section: Discussionmentioning

confidence: 99%

The Transforming Parasite Theileria Co-opts Host Cell Mitotic and Central Spindles to Persist in Continuously Dividing Cells

et al. 2010

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

The Transforming Parasite Theileria Co-opts Host Cell Mitotic and Central Spindles to Persist in Continuously Dividing Cells

et al. 2010

View full text Add to dashboard Cite

show abstract

“…This tick-transmitted parasite of ruminants can transform its host macrophages through chronic de-regulation of host cell signaling pathways. Still incompletely understood host cell transformation processes promote uncontrolled proliferation, long-term survival and parasite dissemination in the host animal [6]. Infected cells can be used as a reversible model of oncogenic transformation [7-12] because the parasite can be eliminated by parasitocidic treatment with the drug Buparvaquone 720c; hence transformation-dependent alterations can be determined and pathways that promote these alterations identified.…”

Section: Introductionmentioning

confidence: 99%

Filopodia and Membrane Blebs Drive Efficient Matrix Invasion of Macrophages Transformed by the Intracellular Parasite Theileria annulata

Baumgärtner

2013

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Recent technical advances have broadened our understanding of processes that govern mammalian cell migration in health and disease but many of the molecular and morphological alterations that precede and accompany movement of cells – in particular in three-dimensional (3D) environments - are still incompletely understood. In this manuscript, using high-resolution and time-lapse microscopy imaging approaches, we describe morphodynamic processes during rounded/amoeboid cell invasion and molecules associated with the cellular invasion structures. We used macrophages infected with the intracellular protozoan parasite Theileria annulata, which causes Tropical Theileriosis in susceptible ruminants such as domestic cattle. T . annulata transforms its host cell that, as a result, acquires many characteristics of human cancer cells including a markedly increased potential to migrate, disseminate and expand in the body of the host animal. Hence, virulence of the disease is associated with the capability of infected cells to disseminate inside the host. Using T . annulata -transformed macrophages as a model system, we described a novel mode of rounded/amoeboid macrophage migration. We show that filopodia-like membrane extensions at the leading edge lead the way and further evolve in blebbing membrane protrusions to promote progressive expansion of the matrix. Associated with focal invasion structures we detected ezrin, radixin, moesin-family proteins and their regulatory kinase MAP4K4. Furthermore, we linked Rho-kinase activity to contractile force generation, which is essential for infected cell motility. Thus, the motility mode of these parasite-transformed macrophages contrasts with those described so far in human macrophages such as the tunneling or mesenchymal modes, which require engulfment, compaction and ingestion of matrix or proteolytic matrix degradation, respectively. Together, our data reveal protrusion dynamics at the leading edge of invading cells in 3D at unprecedented temporal and spatial resolution and suggest a novel mode of rounded/amoeboid invasive cell motility that exploits actin-driven filopodia formation in combination with pressure-driven membrane blebs.

show abstract

“…Theileriosis is a tick‐borne disease caused by Theileria species, which are intracellular apicomplexan parasites (piroplasms) that affect a range of wild and domestic animals, particularly ruminants []. Depending on the species or subspecies of Theileria , clinical signs can include depression, lymph node swelling, tachypnea, dyspnea, hemolytic anemia, jaundice, abortion, stillbirths and metritis, and mortality is common []. Theileria was first detected in cattle in Australia in the 1900s.…”

Section: Introductionmentioning

confidence: 99%

Mutation scanning‐based analysis of Theileria orientalis populations in cattle following an outbreak

Cufos¹,

Jabbar

Carvalho

et al. 2012

Electrophoresis

View full text Add to dashboard Cite

Bovine theileriosis is a tick-borne disease caused by one or more hemoprotozoan parasites of the genus Theileria. In the past, Theileria infection in cattle in Australia was largely asymptomatic and recognized to be associated with Theileria buffeli. However, outbreaks of theileriosis have occurred in beef and dairy cattle in subtropical climatic regions (New South Wales) of Australia. There is also one published report of a recent theileriosis outbreak in a beef farm near Seymour in the southeastern state of Victoria. In order to gain an improved insight into the genetic composition of Theileria populations following this outbreak, we undertook herein an integrated PCR-coupled mutation scanning-sequencing-phylogenetic analysis of sequence variation in part of the major piroplasm surface protein (MPSP) gene within and among samples from cattle involved in the outbreak. Theileria DNA was detected in 89.4% of 94 cattle in the Seymour farm; the genetic analysis showed that the ikeda and chitose genotypes representing the Theileria orientalis complex were detected in 75 and 4.8% of 84 infected cattle, respectively, and that mixed populations of these two genotypes were found in 20.2% of infected cattle. Given unpublished reports of a significant increase in the number of outbreaks in Victoria, future investigations should focus sharply on elucidating the epidemiology of Theileria to subvert the economic impact on the cattle industry in this state. Although used here to explore genetic variation within the T. orientalis complex in Australia, a mutation scanning-based approach has broad applicability to other species of Theileria in other countries.

show abstract

Theileria

Cited by 14 publications

References 112 publications

The Transforming Parasite Theileria Co-opts Host Cell Mitotic and Central Spindles to Persist in Continuously Dividing Cells

The Transforming Parasite Theileria Co-opts Host Cell Mitotic and Central Spindles to Persist in Continuously Dividing Cells

Filopodia and Membrane Blebs Drive Efficient Matrix Invasion of Macrophages Transformed by the Intracellular Parasite Theileria annulata

Mutation scanning‐based analysis of Theileria orientalis populations in cattle following an outbreak

Contact Info

Product

Resources

About