TheHIS4 gene from the yeastKluyveromyces lactis

Freire-Picos, María Ángeles; Hampsey, Michael; Cerdán, M. Esperanza

doi:10.1002/(sici)1097-0061(199805)14:7<687::aid-yea261>3.0.co;2-4

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…TFIIFα has important roles during transcription initiation, elongation and even pausing (Gong et al, 1993;Landick, 2006;Price et al, 1989;Rossignol et al, 1999). Based on its role in different steps of transcription, TFIIFα, either alone or associated with other TFs, might be influencing transcription start site selection (Freire-Picos et al, 2005;Khaperskyy et al, 2008) or preventing RNA PolII (which was not certified by peer review) is the author/funder. All rights reserved.…”

Section: Discussionmentioning

confidence: 99%

Fate transitions in Drosophila neural lineages: a single cell road map to mature neurons.

Reis

Κωνσταντινίδης

et al. 2021

Preprint

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Brain development requires the formation of thousands of neurons and glia and their coordinated maturation to ensure correct circuit formation. Neuronal fate is determined by several layers of gene regulatory networks during neuronal lineage progression. Once specified, neurons must still undergo a critical maturation period, involving the stepwise expression of functional feature genes such as cell surface molecules, ion channels or neurotransmitter receptors. The precise mechanisms that govern neuronal maturation remain however poorly understood. Here, we use single-cell RNA sequencing combined with a conditional genetic strategy to select and analyse neural lineages and their young progeny at a restrictive timepoint to investigate the transcriptional trajectories in young developing secondary neurons in the Drosophila larval brain. Our findings reveal that neuron maturation starts very quickly after neuronal birth, and sub-divide the process of maturation into 3 distinct phases: Phase 1 is composed by immature neurons that have yet to start expressing mRNA of mature neuronal features; Phase 2 includes neurons that start transcribing but not translating maturation markers such as neurotransmitter genes; Phase 3 neurons start translating mature neuronal features, in a coordinated fashion with the animal developmental stage. This dataset represents a complete transcriptomic characterization of the neural lineages generated at this larval stage in the central brain and ventral nerve cord. Its analysis has also allowed for the characterization of a yet undefined transitional state, the immature ganglion mother cells, and has proven useful for the identification of known and novel fate regulators.

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Section: Discussionmentioning

confidence: 99%

Fate transitions in Drosophila neural lineages: a single cell road map to mature neurons.

Reis

Κωνσταντινίδης

et al. 2021

Preprint

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“…The 1-kb fragment obtained by PCR, with OMLPR6 5 0 -TAGGATCCACTG CAAATCAAGC-3 0 and OML15 5 0 -CATGCCCATAAAG GG-3 0 and pM756 [7] as template, was used as the KlHIS4 probe. The KlLEU2 probe was also obtained by PCR, with OMLKL1 5 0 -CCTTAATAGCTTCGTCAG-3 0 and OMLKL2 5 0 -TTTAACGCATCCTACATTTG-3 0 using NRRL-Y1140 genomic DNA as template.…”

Section: Northern Analysesmentioning

confidence: 99%

A stress response related to the carbon source and the absence of KlHAP2 in Kluyveromyces lactis

Lamas-Maceiras

Rodríguez-Torres

Freire-Picos

2010

J Ind Microbiol Biotechnol

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The Kluyveromyces lactis HIS4 gene (KlHIS4) is transcriptionally regulated by the carbon source. The promoter region encompassing positions -238 to -139 is responsible for this regulation according to lacZ reporter assays. Electrophoretic Mobility Shift Assay (EMSA) experiments on KlHIS4 promoter (positions -218 to -213, Fragment 6, F6) show a specific gel-shift band, CS1, whose intensity is carbon-source dependent in K. lactis hap2 (klhap2) knock-out strains. The klhap3 mutation is not able to cause this effect by itself, but the combination of klhap2 and klhap3 mutations has an enhanced effect on CS1 band formation. Introducing a heat shock element (HSE) at the sequence in the F6 fragment (mutated F6, F6*) increases the binding activity in the klhap2 mutant. KlHIS4 mRNA levels in the klhap2 or the double Klhap2/3p mutant do not correlate with the increase in CS1 binding activity, indicating that the factor causing CS1 is acting and only detectable in vitro. EMSA experiments with K. lactis wild-type cells under temperature stress conditions show a band enhancement (Ts1), similar in size to CS1. Cross-competition experiments between F6 and F6* show that F6* competes more efficiently than F6 for both CS1 and Ts1 formation, indicating the involvement of the HSE in the formation of the specific gel-shift bands. Moreover, the similar gel-shift patterns suggest that both bands are caused by the same heat shock-like factor under different stress conditions. Therefore, the enhancement of the CS1 band signal in the klhap2 (and klhap2/3) mutants is due to the increase in heat shock-like factors in the protein extracts from these mutant cells grown in a non-fermentable carbon source. This Klhap2-dependent stress effect was not previously described in K. lactis.

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“…After cloning the KlHIS4 gene by complementation, it was found to encode a protein with a 69% identity and 81% similarity to the multi‐enzymatic protein encoded by the S. cerevisiae HIS4 gene [18]. Herein we report the sequence of the promoter region which is responsible for its transcriptional regulation and necessary in order to allow histidine auxotrophy complementation in S. cerevisiae .…”

Section: Introductionmentioning

confidence: 99%

Kluyveromyces lactis HIS4 transcriptional regulation: similarities and differences to Saccharomyces cerevisiae HIS4 gene

1999

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Sequence analysis of the Kluyveromyces lactis HIS4 (KlHIS4) gene promoter reveals relevant differences in comparison to the Saccharomyces cerevisiae HIS4 homologous gene. Among them are the absence of a Rap1 binding site and the presence of only three putative Gcn4 binding consensus sites instead of the five described in the S. cerevisiae promoter. Since these factors are implicated in the general control, we investigated the transcriptional regulation of the KlHIS4 gene under conditions of amino acid starvation and discovered that the mechanisms previously described for S. cerevisiae HIS4 regulation and related to general control are not functional in K. lactis. The expression analysis of the KlHIS4 gene under phosphate starvation or high adenine supply shows that factors, such as Bas1 or Bas2, involved in the basal control may also operate in a different way in K. lactis. Interestingly, and also in contrast to the HIS4 regulation in S. cerevisiae, we found domains for Nit2-like and yeast-Ap1-like binding sequences. Northern analyses showed transcriptional activation under ammonia starvation and oxidative stress. The EMBL accession number for the KlHIS4 promoter is AJ238494.z 1999 Federation of European Biochemical Societies.

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TheHIS4 gene from the yeastKluyveromyces lactis

Cited by 6 publications

References 19 publications

Fate transitions in Drosophila neural lineages: a single cell road map to mature neurons.

Fate transitions in Drosophila neural lineages: a single cell road map to mature neurons.

A stress response related to the carbon source and the absence of KlHAP2 in Kluyveromyces lactis

Kluyveromyces lactis HIS4 transcriptional regulation: similarities and differences to Saccharomyces cerevisiae HIS4 gene

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