TheH-2L locus and the system of H-2 specificities

Démant, Peter; Néauport–Sautès, Catherine

doi:10.1007/bf01844020

Cited by 28 publications

(19 citation statements)

References 33 publications

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“…A significant lysis was also observed on B10.AKM targets (12%) but not on B10 (4.5%). These data are in accordance with the stronger cross-reaction, described at the serological and CML levels, between H-2.L a and H-2.L q molecules as compared to H-2.L d and H-2.L b (2,16). Surprisingly, adsorption of the CTL on B10 or B10.AKM targets (H-2.28, 29 positive) did not remove the cytotoxicity on C3H.OL targets (10%, P < 0.01) that also express the H-2.28, 29 specificities.…”

Section: Methodssupporting

confidence: 90%

Separate cytotoxic T lymphocyte subsets recognize the different H-2 specificities.

Vázquez¹,

Néauport–Sautès²,

Sénik³

1980

The Journal of Experimental Medicine

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Using a monolayer adsorption technique, the fine specificity of cytotoxic effector T lymphocytes (CTL) generated against allogeneic or semi-allogeneic H-2 haplotypes was investigated. The results show that: (a) CTL reacting with the private specificity expressed on an H-2.K molecule can be separated from those reacting with the public specificities expressed on the same molecule and (b) the CTL that recognize cross-reacting H-2 determinants (public specificities) can also be separated into several subpopulations. These data support the hypothesis that an allogeneic stimulation induces a large number of independent T cell clones that react with H-2 determinants.

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Section: Methodssupporting

confidence: 90%

Separate cytotoxic T lymphocyte subsets recognize the different H-2 specificities.

Vázquez¹,

Néauport–Sautès²,

Sénik³

1980

The Journal of Experimental Medicine

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“…We presume that in the sera used by Lemonnier et al (1975), besides anti-L28, anti-D28 antibodies were present and possibly also antibodies against other short public specificities of H-2Dd, while in our sera absorbed on BALBI C -H -~~~' cells only non-cross-reacting anti-L28 antibodies were present. Anti-H-2.28 antibodies reactive with H--2Dd molecules were demonstrated in our previous paper (Dkmant et al 1978).…”

Section: Independence Of H-2d and H-2l Molecules On The Cell Surface mentioning

confidence: 70%

“…The evidence of H-2.28 family sites in both the K and D regions was provided by Snell et al (1974) who reported that anti-H-2.28 antibodies formed against D region products react with H-2.28 of H-2K. Using the antigen redistribution method we could demonstrate that the same anti-H-2.28 antibodies which react with H-2K or H-2D molecules also bind to H-2L molecules, which shows that antigenically related determinants of H-2.28 family can be present on all three types of H-2 molecules (Dkmant et al 1978). If the pro-posed allelism of the families H-2.28 and H-2.1 is true, the specificities of either one or the other of these two families have to be present on H-2L molecules of all H-2 haplotypes.…”

Section: Percent Cells Where Tritc Labeling Outside Fitc Caps Is Targmentioning

confidence: 85%

“…The question whether these molecules represent allelic products of the same locus, or products of different loci is difficult to answer conclusively, because of uncertainties concerning the genetic basis of the H-2 polymorphism (i.e. true allelism versus polymorphism through control of expression) (Dkmant et al 1978), and possible participation of two or more different portions of genome in determination of the antigenic characteristics of a single molecule. The general properties common to H-2L molecules of different haplotypes, however, do not indicate that they are actually products of different loci any more than e.g.…”

Section: Independence Of H-2d and H-2l Molecules On The Cell Surface mentioning

confidence: 99%

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H–2L: Demonstration of Four New Allelic Products and Independence of H–2D and H–2L Molecules

1979

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The H–2L molecules were detected for the first time in the Dd region products using antisera against the public specificity H‐2.28. This specificity was analyzed because its presence in K as well as in D region products and its apparent allelism with another public specificity, H‐2.1, indicated that these two specificities may have a special position in the H‐2 system. This was corroborated by subsequent identification of H‐2L molecules in Dq and Dk products using anti‐H‐2.28 and anti‐H‐2.1 sera, respectively, while none of the other previously known public or private specificities was detected on H‐2L molecules. We tested the products of four D region alleles which had not been analyzed previously. In each of them we identified two distinct types of molecules: H–2D, which reacts with sera against the D region private specificity, and H–2L, which does not react with these sera, but which is detectable either by anti‐H‐2.28 sera (H‐2Lb, H‐2Lf, H‐2Ls) or by anti‐H‐2.1 sera (H‐2Ldx). This increases the number of identified H‐2L alleles to seven (five H‐2.8+, two H‐2.1+). No association between H‐2D and H‐2L molecules on the cell surface was detected in capping experiments.

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“…A third H-2 locus, H-2L, which shares public but not private specificities with H-2D alloantigens, has recently been described (39,40). Hence, antisera used to immunoprecipitate H-2D molecules could also detect H-2L alloantigens.…”

Section: Comparison Of the Arginine T~yptic Peptides Of Qa-2 And H-2 mentioning

confidence: 99%

Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene.

Soloski¹,

Uhr²,

Flaherty³

et al. 1981

The Journal of Experimental Medicine

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The Tla region located on the murine 17th chromosome controls several serologically defined cell surface antigens. These antigens, referred to as Qa-1-5 and TL, are expressed on a variety of hematopoeitic cell populations. In the present studies we have immunoprecipitated isotopically labeled Qa-2 and H-2 molecules from mitogen-stimulated B6 spleen cells. Sequential immunoprecipitation experiments have shown that the determinants recognized by alpha Qa-2, alpha H-2Kb, and alpha H-2Db alloantisera reside on separate molecular species. Comparative mapping of the arginine-labeled tryptic peptides from Qa-2, H-2Kb, and H-2Db molecules indicate that Qa-2 is structurally distinct but that there is considerable structural homology; 21-43% of the Qa-2 peptides co-chromatograph with peptides derived from H-2Db and H-2Kb, respectively. Similar levels of homology are observed when Qa-2 is compared with H-2Kk or H-2Dd. The results show that the Qa-2 alloantigen is encoded by a locus separate from the loci encoding H-2K or H-2D alloantigens, but that the Qa-2, H-2K, and H-2D alloantigens are sufficiently related at the primary structural level to indicate that they evolved from a common primordial gene.

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TheH-2L locus and the system of H-2 specificities

Cited by 28 publications

References 33 publications

Separate cytotoxic T lymphocyte subsets recognize the different H-2 specificities.

Separate cytotoxic T lymphocyte subsets recognize the different H-2 specificities.

H–2L: Demonstration of Four New Allelic Products and Independence of H–2D and H–2L Molecules

Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene.

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