Thearubigins protect against acetaminophen-induced hepatic and renal injury in mice: biochemical, histopathological, immunohistochemical, and flow cytometry study

Murad, Hussam; Habıb, Hamid; Kamel, Yasser Mostafa; Alsayed, Salah A.; Shakweer, Marwa M; Elshal, Mohamed F.

doi:10.3109/01480545.2015.1070170

Cited by 26 publications

(27 citation statements)

References 36 publications

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“…This suggests that FA may play a protective role as an antioxidant in APAP toxicity. It is reported that different doses of APAP administration causes kidney damage such as tubular vacuolization, lymphocyte infiltration and tubular dilatation [40][41][42]. In our study, hyperemia in vessels, dilatation in tubules and disruptions in tubular epithelium were observed in APAP group.…”

Section: Discussionsupporting

confidence: 59%

“…Studies indicated that APAP causes severe liver damage such as massive neutrophilic and lymphocytic infiltration, intense coagulative necrosis, cytoplasmic vacuoles, vacuolar degeneration in hepatocytes, congestion of central vein and portal vein in the liver [23,40]. In our study, APAP group showed dilatation of the central veins, centrilobular necrosis, congestion and vacuolar degeneration, as well as loss of glycogen storage ability of hepatic parenchyma.…”

Section: Discussionsupporting

confidence: 57%

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The potential protective role of folic acid against acetaminophen-induced hepatotoxicity and nephrotoxicity in rats

2021

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Folic acid (FA), is a group B vitamin, has high reactive oxygen radicals quenching ability, resulting in protection against oxidative damage in aerobic cell. Acetaminophen (N-acetyl-paminophenol, APAP) is a nonsteroidal anti-inflammatory drug, and can promote oxidative damage in liver and kidney tissues. The aim of this study was to investigate whether folic acid has protective effects on oxidative liver and kidney injury caused by experimental APAP toxication. Forty female Sprague dawley rats were divided into 5 groups; control, APAP, FA, APAP+FA, and APAP+N-acetylcysteine (NAC) groups. APAP toxication was induced by oral gavage (3 g/kg bodyweight). FA (20 mg/kg bodyweight) and NAC (150 mg/kg bodyweight) were given by oral gavage to the specified groups. Oxidant and antioxidant parameter were determined in liver and kidney tissues. In addition, the liver and kidney tissues were histological evaluated. When compared with APAP group, superoxide dismutase (SOD) and catalase activities and glutathione levels were statistically higher, malondialdehyde (MDA) level and myeloperoxidase activity (except liver tissue) were statistically lower in both APAP+FA and APAP+NAC. Liver and kidney MDA level and kidney SOD activity were significantly lower in APAP+NAC group compared with APAP+FA group. Co-administration of NAC with APAP was found to provide protection, but hepatic cords were defective in some places and some glomerular tubules also had dilatation. Necrotic areas was reduced in the liver and the glomerular structure was in good condition in the APAP+FA group. As a result, FA might have a protective effect against APAP-induced hepato-nephrotoxicity and oxidative stress in rat.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionsupporting

confidence: 57%

The potential protective role of folic acid against acetaminophen-induced hepatotoxicity and nephrotoxicity in rats

2021

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“…8, which shows large signals attributable to the A-ring methine carbons. Kuhnert reported that the molecular weights of TRs are less than 2000 Da 108) ; however, this is also still contentious, 96,112) and the discrepancy may be caused by the different extraction solvents and preparation methods that are used for preparation of TRs.…”

Section: Thearubiginsmentioning

confidence: 99%

Production Mechanisms of Black Tea Polyphenols

Tanaka

Matsuo

2020

Chem. Pharm. Bull.

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Black tea accounts for 70-80% of world tea production, and the polyphenols therein are produced by enzymatic oxidation of four tea catechins during tea fermentation. However, only limited groups of dimeric oxidation products, such as theaflavins, theasinensins, and theacitrins, have been isolated from black tea and chemically characterized. This is largely because of the complexity and heterogeneity of the oxidation products. To determine structures and production mechanisms of uncharacterized black tea polyphenols, in vitro model fermentation experiments using pure catechins and polyphenol oxidase have been applied, and basic oxidation mechanisms have been established. Contemporary methods, such as LC-MS, are also effective to identify catechin oxidation products in black tea. Despite ongoing efforts, almost 60% of the solids in black tea infusion remain uncharacterized. These compounds include the so-called thearubigins, which are a heterogeneous mixture of uncharacterized catechin oxidation products with oligomeric structures. This review summarizes the current knowledge of the production mechanisms of representative black tea polyphenols and presents recent progress in characterization of thearubigins.

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“…Numerous studies have reported the biological activities of TRs. Murad and colleagues reported that TRs protected against acetaminophen-induced hepatotoxicity and nephrotoxicity in mice [24], and improved the sildenafil-induced delayed gut motility in mice [25]. Halder et al found that TRs have significant antimutagenic and anticlastogenic effects in Salmonella assay in vitro and in vivo in bone marrow cells of mice [26].…”

Section: Introductionmentioning

confidence: 99%

A new method to prepare and redefine black tea thearubigins

Wang

Zhang

et al. 2018

Journal of Chromatography A

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Thearubigins (TRs) are the major components of black tea, which are formed during the fermentation reactions. Although anti-inflammatory and anti-cancer activities of TRs have been reported, the prepared TRs according to the literature methods still contain many floating peaks. It is puzzling whether the observed activities are from TRs or these floating peaks. Thus, it is urgent to develop a method to prepare pure TRs and redefine them. In the present study, we developed a new method, the combination of caffeine precipitation and Sephadex LH-20 column chromatography, to prepare pure TRs. The floating peaks on the hump of the crude TRs were removed, and pure TRs were prepared. The chemical profile of the floating peaks was established using LC/MS, and the major compounds in this fraction were identified as apigenin glycosides, quercetin glycosides, kaempferol glycosides, theaflavins, theasinensin, and galloylglucoses based on the analysis of their tandem mass spectra and in comparison with literature data. This study will pave the way to further study the chemistry and biological activities of TRs and the health effects of black tea consumption.

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Thearubigins protect against acetaminophen-induced hepatic and renal injury in mice: biochemical, histopathological, immunohistochemical, and flow cytometry study

Abstract: Thearubigins protected against acetaminophen-induced hepatotoxicity and nephrotoxicity in mice possibly through their antioxidant activity.

Cited by 26 publications

References 36 publications

The potential protective role of folic acid against acetaminophen-induced hepatotoxicity and nephrotoxicity in rats

The potential protective role of folic acid against acetaminophen-induced hepatotoxicity and nephrotoxicity in rats

Production Mechanisms of Black Tea Polyphenols

A new method to prepare and redefine black tea thearubigins

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