The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na+/K+ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover

Cheng, Bizhen; Li, Zhou; Li, Liang; Cao, Yiqin; Zeng, Weizhong; Zhang, Xinquan; Ma, Xiao; Huang, Linkai; Nie, Gang; Liu, Wei; Peng, Yan

doi:10.3390/ijms19092520

Cited by 108 publications

(86 citation statements)

References 54 publications

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“…The inhibition of genes encoding seed-specific dehydrins caused significant decreases in seed germination and the longevity of Arabidopsis during storage at low moisture and salt stress [ 41 ]. During seed germination, exogenous γ-aminobutyric acid effectively alleviated salt damage and improved growth related to dehydrin accumulation and dehydrin-encoded gene expression in white clover [ 42 ]. Our current results showed that transcript levels of Y2K and Y2SK were significantly up-regulated by water stress, while dehydrin b expression was significantly down-regulated under drought stress, implying that different types of dehydrins might have different responses to water stress during seed germination.…”

Section: Discussionmentioning

confidence: 99%

Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress

Cao

Cheng

et al. 2018

IJMS

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This study was designed to examine the effects of NaCl pretreatment on the seed germination of white clover (Trifolium repens cv. Ladino) under water stress induced by 19% polyethylene glycol (PEG) 6000. Lower concentrations of NaCl (0.5, 1, and 2.5 mM) pretreatment significantly alleviated stress-induced decreases in germination percentage, germination vigor, germination index, and radicle length of seedlings after seven days of germination under water stress. The soaking with 1 mM of NaCl exhibited most the pronounced effects on improving seed germination and alleviating stress damage. NaCl-induced seeds germination and growth could be associated with the increases in endogenous gibberellic acid (GA) and indole-3-acetic acid (IAA) levels through activating amylases leading to improved amylolysis under water stress. Seedlings pretreated with NaCl had a significantly lower osmotic potential than untreated seedlings during seed germination, which could be related to significantly higher soluble sugars and free proline content in NaCl-treated seedlings under water stress. For antioxidant metabolism, NaCl pretreatment mainly improved superoxide dismutase, peroxidase, ascorbate peroxidase, and glutathione reductase activities, transcript levels of FeSOD, APX, and DHAR, and the content of ascorbic acid, reduced glutathione, and oxidized glutathione during seed germination under water stress. The results indicated that seeds soaking with NaCl could remarkably enhance antioxidant metabolism, thereby decreasing the accumulation of reactive oxygen species and membrane lipid peroxidation during germination under water stress. In addition, NaCl-upregulated dehydrin-encoded genes SK2 expression could be another important mechanism of drought tolerance during seeds germination of white clover in response to water stress.

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Section: Discussionmentioning

confidence: 99%

Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress

Cao

Cheng

et al. 2018

IJMS

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“…Besides its function in maintaining carbon-nitrogen balance, GABA is involved in responses to different types of abiotic stress in plants. Increased concentrations of GABA were detected in conditions of hypoxia, drought, salinity and low or high temperatures, and its role as effective osmolyte and in ROS scavenging is well-known [79].…”

Section: Main Salt Tolerance Mechanisms In the Investigated Speciesmentioning

confidence: 99%

Insights on Salt Tolerance of Two Endemic Limonium Species from Spain

et al. 2019

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We have analysed the salt tolerance of two endemic halophytes of the genus Limonium, with high conservation value. In the present study, seed germination and growth parameters as well as different biomarkers—photosynthetic pigments, mono and divalent ion contents—associated to salt stress were evaluated in response to high levels of NaCl. The study was completed with an untargeted metabolomics analysis of the primary compounds including carbohydrates, phosphoric and organic acids, and amino acids, identified by using a gas chromatography and mass spectrometry platform. Limonium albuferae proved to be more salt-tolerant than L. doufourii, both at the germination stage and during vegetative growth. The degradation of photosynthetic pigments and the increase of Na+/K+ ratio under salt stress were more accentuated in the less tolerant second species. The metabolomics analysis unravelled several differences between the two species. The higher salt tolerance of L. albuferae may rely on its specific accumulation of fructose and glucose under high salinity conditions, the first considered as a major osmolyte in this genus. In addition, L. albuferae showed steady levels of citric and malic acids, whereas the glutamate family pathway was strongly activated under stress in both species, leading to the accumulation of proline (Pro) and γ-aminobutyric acid (GABA).

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“…By treating corn seeds with Penicillium sp. or Mn/Zn added to the film coating treatment, Gómez-Muñoz et al (2018) found that those treatments alleviated the negative effects of cold stress. However, studies involving the use of film coating with the addition of amino acids are not very diffused in the carrot seed treatment literature, been necessary to study the suitable rate to treat the seeds in order to obtain the best results provided from the amino acids.…”

Section: Discussionmentioning

confidence: 92%

“…The use of aminoacids like BABA and GABA added to film coating technic has never been tested by researches, even though there are results comproving the effect of micronutrients, microoganisms or biostimulants added to the coating and its positive effect on seedling tolerance to thermal stress (BRADÁCOVÁ et al, 2016;GÓMEZ-MUÑOZ et al, 2018). Film coating seeds with amino acids is a viable alternative in the availability of these products in a way that provides greater technical efficiency in their use, and studies involving carrot seed treatment and its effect on seed germination and emergence is a relevant subject in order to improve plant standability and early growth development.…”

Section: Biostimulants and Seed Treatment Improving Crop Stress Tolermentioning

confidence: 99%

“…According to Bradácová et al (2016) when a film coating is applied to the seed and there are on its products that can help in nutrient uptake and root growth, mitigating the effect of cold stress can be achieved. In the same way, Gómez-Muñoz et al (2018) studied the effect of three seed treatments on cold stress tolerance of corn seeds and found out that the addition of Mn/Zn and inoculation with Penicillium reduced the adverse effects of cold stress.…”

Section: Introductionmentioning

confidence: 99%

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Seed treatment with aminobutyric acids improve carrot seed germination and vigor under thermal stress conditions

Neto¹,

dos²

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Seed treatment with aminobutyric acids improve carrot seed germination and vigor under thermal stress conditions Carrots are plants adapted to different growing conditions, becoming one of the most cultivated vegetables in the world. In Brazil, it has been cultivated both in winter and summer, but extreme weather can make farmers face problems related to seed germination and plant early development, resulting in the reduction of stand and yield in the productive systems. In the vegetative stage, severe thermal stress can result in retarded growth, chlorosis and leaf necrosis, senescence and even vegetable death. At the cellular level, heat stress can lead to membrane damage, protein denaturation, inactivation of mitochondrial and chloroplast enzymes, impaired protein and carbohydrate synthesis, protein degradation, new protein synthesis, and inhibited carbon metabolism. Making crops tolerants to environmental stress are challenges faced by classical breeding and transgenic approaches and different methods are routinely experimented and applied to enhance the productivity and stress tolerance of crops. One of the simplest ways of giving tolerance to sensitive plant species involves the use of biomolecules with regulatory or stimulating roles in stressed plants, the biostimulants. Biostimulants are defined as substances that contain compounds as microorganisms, microbial inoculants, humic acids, fulvic acids, protein hydrolysates, amino acids, and seaweed extracts that stimulate natural process of plant growth. Some non-protein amino acids, such as γ-aminobutyric acid (GABA), β-aminobutyric acid (BABA) have been commonly studied by the ability of confering tolerance to abiotic stress. With this, the objective of this study was to evaluate the application of a film coating in carrot seeds composed with different rates of GABA and BABA and evaluate it effect on seed germination, emergence and vigor under heat and cold stress.

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The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na+/K+ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover

Cited by 108 publications

References 54 publications

Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress

Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress

Insights on Salt Tolerance of Two Endemic Limonium Species from Spain

Seed treatment with aminobutyric acids improve carrot seed germination and vigor under thermal stress conditions

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