The β2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney

Abstract

We have recently shown that nitric oxide activates the beta2 subunit of soluble guanylyl cyclase. In the present study, we show developmental regulation of this subunit. Analysis of mRNA expression by RT-PCR and RNase protection analysis in kidneys revealed no expression of the beta2 subunit in neonatal and strong expression in adult rats. A reciprocal regulation with much lower expression levels was observed in rat lung. Further examination of kidneys from 3, 6, 16, 22, 25, 31 and 36-day-old rats showed that significant expression appears between postnatal day 16 and 22. Isolation of the rat beta2 promoter by genome walking and cloning into a reporter gene vector showed promoter activity for the sense but not the antisense construct providing an in vitro assay for further analysis of the developmental beta2 subunit regulation.

High-salt diets during pregnancy increases renal vascular reactivity due to altered soluble guanylyl cyclase-related pathways in rat offspring

“…One previous study [23], focused on the aorta of 12-week-old HS offspring, showed that expression of sGC β1 was reduced while sGC β2 did not change. sGC β2 was reported to be richly expressed in the kidney [27,28]. Our results suggested that the reduction of sGC β1 and sGC β2 in the HS interlobar arteries could be the cause for the reduced ΔpD 2 in the HS offspring.…”

Soluble guanylyl cyclase during postnatal porcine pulmonary maturation

“…In the rat heart and kidney, sGC increased postnatally (7,9). In contrast, sGC expression in carotid and cerebral arteries was higher in 3-to 7-day-old lambs compared with adult sheep (37).…”

Expression of inducible nitric oxide synthase messenger RNA, but not guanylate cyclase messenger RNA, depends on gestational age in rat myometrium

“…The sGC a1 subunit is the most abundant a subunit in adult human tissues, including cardiovascular tissues 23 . Analysis of the tissue distribution of the b2 subunit has been difficult, owing to low mRNA concentrations of this subunit compared with other sGC subunits 24 . The sGC b2 subunit in uterine tissue was the lowest of the three subunits of mRNA expressed in our investigation.…”