The β Subunit of Eukaryotic Translation Initiation Factor 2 Binds mRNA through the Lysine Repeats and a Region Comprising the C<sub>2</sub>-C<sub>2</sub> Motif

Laurino, Jomar Pereira; Thompson, Glória M.; Pacheco, Eliza; Castilho, Beatriz A.

doi:10.1128/mcb.19.4.3224

Cited by 24 publications

(48 citation statements)

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“…Both motifs have been implicated in mRNA binding and could aid in codon-anticodon interactions. (17) The lysine repeats furthermore mediate the mutually exclusive interaction of eIF5 and the e-subunit of eIF2B with eIF2b. (18) The a-subunit of eIF2 functions predominantly as a regulator of translation initiation.…”

Section: Introductionmentioning

confidence: 99%

Starting the protein synthesis machine: eukaryotic translation initiation

2003

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SummaryThe final assembly of the protein synthesis machinery occurs during translation initiation. This delicate process involves both ends of eukaryotic messenger RNAs as well as multiple sequential protein-RNA and protein-protein interactions. As is expected from its critical position in the gene expression pathway between the transcriptome and the proteome, translation initiation is a selective and highly regulated process. This synopsis summarises the current status of the field and identifies intriguing open questions.

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Section: Introductionmentioning

confidence: 99%

Starting the protein synthesis machine: eukaryotic translation initiation

2003

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“…In the archaeal aIF2 complex, the C-terminal zinc-binding domain of aIF2b packs against the central a-b domain (Sokabe et al 2006;Yatime et al 2007). While the function of the zinc-binding domain has not been resolved, removal of this domain impairs RNA binding to isolated yeast eIF2b (Laurino et al 1999) and confers a dominant Gcd 2 and recessive lethal phenotype (CastilhoValavicius et al 1992), while point mutations confer a dominant Sui 2 phenotype CastilhoValavicius et al 1992).…”

Section: Ternary Complex Formationmentioning

confidence: 99%

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

2016

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In this review, we provide an overview of protein synthesis in the yeast Saccharomyces cerevisiae. The mechanism of protein synthesis is well conserved between yeast and other eukaryotes, and molecular genetic studies in budding yeast have provided critical insights into the fundamental process of translation as well as its regulation. The review focuses on the initiation and elongation phases of protein synthesis with descriptions of the roles of translation initiation and elongation factors that assist the ribosome in binding the messenger RNA (mRNA), selecting the start codon, and synthesizing the polypeptide. We also examine mechanisms of translational control highlighting the mRNA cap-binding proteins and the regulation of GCN4 and CPA1 mRNAs.

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“…30) As mentioned above, SUI1 and SUI3 encode the translation initiation factors eIF1 and eIF2β, respectively, which are involved in the recognition of initiation codons on mRNAs. [24][25][26] Despite the current lack of direct evidence, we speculate that the mutation of these factors might cause the His + phenotype by promoting translation of the His3 protein from codons, other than the AUG located within the region near the promoter. Given that mutations in the specific genes mentioned above were repeatedly isolated (note that more sui1 and sui3 mutants were isolated, but excluded from further analyses by testing whether plasmids containing the SUI1 or SUI3 gene suppressed the ts and His + phenotypes of the mutant strains), the genetic screen appeared to be saturated.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the endogenous SUI1 gene of these three strains contained a mutation within the open reading frame (ORF) (A63T, A63V, or G66S; Table 1), indicating that their His + phenotype was caused by these mutations. Similarly, a mutation in the SUI3 gene (L254Q ; Table 1), which encodes the β-subunit of eIF2, 25,26) was found to underlie the ts and His + phenotypes of the remaining ts strain.…”

Section: Resultsmentioning

confidence: 99%

Both HMG boxes in Hmo1 are essential for DNA binding in vitro and in vivo

Higashino

Shiwa

Yoshikawa

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Hmo1, a member of the high mobility group B family proteins in Saccharomyces cerevisiae, associates with the promoters of ribosomal protein genes (RPGs) to direct accurate transcriptional initiation. Here, to identify factors involved in the binding of Hmo1 to its targets and the mechanism of Hmo1-dependent transcriptional initiation, we developed a novel reporter system using the promoter of the RPG RPS5. A genetic screen did not identify any factors that influence Hmo1 binding, but did identify a number of mutations in Hmo1 that impair its DNA binding activity in vivo and in vitro. These results suggest that Hmo1 binds to its target promoters autonomously without any aid of additional factors. Furthermore, characterization of Hmo1 mutants showed that the box A domain plays a pivotal role in DNA binding and may be required for the recognition of structural properties of target promoters that occur in native chromatin.

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The β Subunit of Eukaryotic Translation Initiation Factor 2 Binds mRNA through the Lysine Repeats and a Region Comprising the C₂-C₂ Motif

Cited by 24 publications

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Starting the protein synthesis machine: eukaryotic translation initiation

Starting the protein synthesis machine: eukaryotic translation initiation

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

Both HMG boxes in Hmo1 are essential for DNA binding in vitro and in vivo

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The β Subunit of Eukaryotic Translation Initiation Factor 2 Binds mRNA through the Lysine Repeats and a Region Comprising the C2-C2 Motif

Cited by 24 publications

References 0 publications

Starting the protein synthesis machine: eukaryotic translation initiation

Starting the protein synthesis machine: eukaryotic translation initiation

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

Both HMG boxes in Hmo1 are essential for DNA binding in vitro and in vivo

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The β Subunit of Eukaryotic Translation Initiation Factor 2 Binds mRNA through the Lysine Repeats and a Region Comprising the C₂-C₂ Motif