The β-subunit composition of nicotinic acetylcholine receptors in the neurons of the guinea pig inferior mesenteric ganglion

Functional role of the nicotinic arm of the acetylcholine regulatory axis in human B-cell lines

“…16,17 The highest amount of antibody binding was observed in Daudi cells and the lowest in REH cells ( Figure 1A). Because Daudi cells express α2, but not α4, subunit, 3 the reactivity of α2/α4 antibody was attributed to α2 nAChR.…”

“…The antibodies used in this study were raised against short synthetic fragments of the rat nAChR subunits α4, α7, β2, or β4 and shown to bind corresponding nAChR subunits in rat and guinea-pig autonomic ganglia 16,17 and mouse B lymphocytes. 10,11 The sequences used to generate the α4, β2, and β4 antibodies are identical between human and rat.…”

“…The antibody concentration was selected according to ELISA with corresponding antigenic peptides, as detailed elsewhere. 16,17 After a thorough wash with PBS, the cells were stained with PE-labeled streptavidin for 15 min, washed by centrifugation and analyzed on the EPICS-XL flow cytometer (Coulter-Beckman, Fullerton, CA) using appropriate software. The cell aliquots stained with FITC-labeled anti-CD19 and PE-labeled anti-CD22 were analyzed similarly in a flow cytometer.…”

Nicotinic acetylcholine receptors: specific antibodies and functions in humoral immunity

“…Since the peptides were quite different in amino acid composition, the resulting antibodies were assumed to distinguish between homologous subunits and to selectively influence the functions of certain nAChR subtypes. The specificity and functional properties of these antibodies were tested in the established cell lines and in the neurons of several autonomic ganglia expressing corresponding nAChRs by means of immunocytochemistry, immunoenzyme techniques like Western blots and Cell elISA, fluorescent flow cytomet ry and electrophysiology approaches to study both acetylcholine-induced membrane currents and excitatory post-synaptic potentials (ePSP) [14][15][16]. It was demonstrated that the antibodies were both subunit-specific and functionally potent: they blocked membrane currents induced by externally applied acetylcholine [14], as well as electric potentials stimulated through excitation of nerve endings [15].…”

Caudal mesenteric ganglion in the sheep – macroanatomical and immunohistochemical study

“…The physiology and pathophysiology of the CaMG and corresponding plexus is widely discussed in the literature (Melman and Kapskya 1960, Nozdrachev 1968, Kostina 1971, Komissarov and Krivobok 1975, Archakova 1979, Kaleczyc et al 1995, Sann et al 1995, Berthoud and Powley 1996, Elfvin et al 1997, Ribeiro et al 2000a, Ribeiro et al 2000b, Pasichnichenko and Skok 2002, Gagliardo et al 2003, Koval et al 2004, Gagliardo et al 2005, Pidsudko et al 2011. Unfortunately, the lack of detailed descriptions of these structures in domestic mammals, besides some general comments provided by anatomical textbooks (Dyce et al 2002, Nickel et al 2004, Koenig and Liebich 2007, have partly prompted us to undertake anatomical studies regarding the ovine CaMG.…”