The β-lactamases of Gram-negative bacteria and their rôle in resistance to β-lactam antibiotics

Sykes, R. B.; Matthew, Margaret

doi:10.1093/jac/2.2.115

Cited by 529 publications

(295 citation statements)

References 0 publications

Supporting

Mentioning

279

Contrasting

Unclassified

Order By: Relevance

“…1A). This enzymatic deactivation occurs both in the periplasmic space of the cell and in the extracellular medium (28,31) (SI Appendix, Fig. S1), thus leading to immediate benefits to the sensitive partner (19,32).…”

Section: Resultsmentioning

confidence: 99%

Oscillatory dynamics in a bacterial cross-protection mutualism

Yurtsev

Conwill

Gore

2016

Proc. Natl. Acad. Sci. U.S.A.

127

View full text Add to dashboard Cite

Cooperation between microbes can enable microbial communities to survive in harsh environments. Enzymatic deactivation of antibiotics, a common mechanism of antibiotic resistance in bacteria, is a cooperative behavior that can allow resistant cells to protect sensitive cells from antibiotics. Understanding how bacterial populations survive antibiotic exposure is important both clinically and ecologically, yet the implications of cooperative antibiotic deactivation on the population and evolutionary dynamics remain poorly understood, particularly in the presence of more than one antibiotic. Here, we show that two Escherichia coli strains can form an effective crossprotection mutualism, protecting each other in the presence of two antibiotics (ampicillin and chloramphenicol) so that the coculture can survive in antibiotic concentrations that inhibit growth of either strain alone. Moreover, we find that daily dilutions of the coculture lead to large oscillations in the relative abundance of the two strains, with the ratio of abundances varying by nearly four orders of magnitude over the course of the 3-day period of the oscillation. At modest antibiotic concentrations, the mutualistic behavior enables long-term survival of the oscillating populations; however, at higher antibiotic concentrations, the oscillations destabilize the population, eventually leading to collapse. The two strains form a successful cross-protection mutualism without a period of coevolution, suggesting that similar mutualisms may arise during antibiotic treatment and in natural environments such as the soil.

show abstract

Section: Resultsmentioning

confidence: 99%

Oscillatory dynamics in a bacterial cross-protection mutualism

Yurtsev

Conwill

Gore

2016

Proc. Natl. Acad. Sci. U.S.A.

127

View full text Add to dashboard Cite

show abstract

“…This excludes, respectively, a resistance due to nucleotidyltransferase and DNA gyrase. In view of this, the existence of only one mechanism due to a production of constitutively plasmid-mediated penicillinases can be assumed [13].…”

Section: Susceptibility Testingmentioning

confidence: 99%

ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

et al. 1997

View full text Add to dashboard Cite

Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transeonjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transeonjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to P-lactamase point-mutations.

show abstract

“…Several authors have proposed formal classifications (e.g. Sawai, Kanno and Tsukamoto, 1982) but the most extensive and systematic scheme is that proposed by Richmond and Sykes (1973) and expounded at length by Sykes and Matthew (1976). In a recent note, Sykes (1982) argues for the continued value of this scheme.…”

Section: ~-Lactalnqses: Inolecular Studies 225mentioning

confidence: 99%

“…Classes III and IV of this scheme both contain enzymes of broad specificityã nd the distinction between them appears, again, to be made mainly on the basis that Class III but not Class IV enzymes are inhibited by cloxacillin. The addition to the classification scheme made by Sykes and Matthew (1976) was to point out that the enzymes in Classes III and V are plasmid..borne and the rest are chromosomal.…”

Section: Richmond and Sykes Classu:icationmentioning

confidence: 99%

ß-Lactamases: Molecular Studies

Coulson

1985

Biotechnology and Genetic Engineering Reviews

View full text Add to dashboard Cite

The β-lactamases of Gram-negative bacteria and their rôle in resistance to β-lactam antibiotics

Cited by 529 publications

References 0 publications

Oscillatory dynamics in a bacterial cross-protection mutualism

Oscillatory dynamics in a bacterial cross-protection mutualism

ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

ß-Lactamases: Molecular Studies

Contact Info

Product

Resources

About