The β-Lactamase Gene Regulator AmpR Is a Tetramer That Recognizes and Binds the d-Ala-d-Ala Motif of Its Repressor UDP-N-acetylmuramic Acid (MurNAc)-pentapeptide

Vadlamani, Grishma; Thomas, Misty D.; Patel, Trushar R.; Donald, Lynda J.; Reeve, Thomas M.; Stetefeld, Jörg; Standing, Kenneth G.; Vocadlo, David J.; Mark, Brian L.

doi:10.1074/jbc.m114.618199

Cited by 67 publications

(84 citation statements)

References 66 publications

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“…Under normal conditions, most anhMP enter the cytoplasm through a permease, AmpG and are efficiently processed by enzymes including the β-N-acetylglucosaminidase, NagZ and AmpD, for recycling into de novo PG biosynthesis ( Figure 1). If anhMP that enter the cytoplasm are not processed (as is the case in an ampD mutant), they accumulate, bind to AmpR -thereby displacing the UDP-MurNAc-pentapeptides that otherwise maintain AmpR in a repressor conformation -and it then a ctivates AmpC expression ( Figure 1) [7,69].…”

Section: Cell-wall Metabolism and Ampc β-Lactamase Inductionmentioning

confidence: 97%

Pseudomonas Aeruginosa : Targeting Cell-Wall Metabolism for New Antibacterial Discovery and Development

Lamers

Burrows

2016

Future Med. Chem.

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Pseudomonas aeruginosa is a leading cause of hospital-acquired infections and is resistant to most antibiotics. With therapeutic options against P. aeruginosa dwindling, and the lack of new antibiotics in advanced developmental stages, strategies for preserving the effectiveness of current antibiotics are urgently required. β-Lactam antibiotics are important agents for treating P. aeruginosa infections, thus, adjuvants that potentiate the activity of these compounds are desirable for extending their lifespan while new antibiotics - or antibiotic classes - are discovered and developed. In this review, we discuss recent research that has identified exploitable targets of cell-wall metabolism for the design and development of compounds that hinder resistance and potentiate the activity of antipseudomonal β-lactams.

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Section: Cell-wall Metabolism and Ampc β-Lactamase Inductionmentioning

confidence: 97%

Pseudomonas Aeruginosa : Targeting Cell-Wall Metabolism for New Antibacterial Discovery and Development

Lamers

Burrows

2016

Future Med. Chem.

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“…1) and is a hallmark of the LTTR family (22,(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80). A recent study using nondenaturing mass spectrometry has illustrated for the first time that an LTTR tetramer binds four molecules of its effector in a stepwise pattern while bound to DNA (81). Binding of the effector produces a change in the angle at which an LTTR bends the promoter DNA to which it is bound (17,19).…”

Section: Postranslational Regulation Of Cbbr Function: the Role Of Efmentioning

confidence: 99%

CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation

Dangel

Tabita

2015

J Bacteriol

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Biological carbon dioxide fixation is an essential and crucial process catalyzed by both prokaryotic and eukaryotic organisms to allow ubiquitous atmospheric CO 2 to be reduced to usable forms of organic carbon. This process, especially the CalvinBassham-Benson (CBB) pathway of CO 2 fixation, provides the bulk of organic carbon found on earth. The enzyme ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (RubisCO) performs the key and rate-limiting step whereby CO 2 is reduced and incorporated into a precursor organic metabolite. This is a highly regulated process in diverse organisms, with the expression of genes that comprise the CBB pathway (the cbb genes), including RubisCO, specifically controlled by the master transcriptional regulator protein CbbR. Many organisms have two or more cbb operons that either are regulated by a single CbbR or employ a specific CbbR for each cbb operon. CbbR family members are versatile and accommodate and bind many different effector metabolites that influence CbbR's ability to control cbb transcription. Moreover, two members of the CbbR family are further posttranslationally modified via interactions with other transcriptional regulator proteins from two-component regulatory systems, thus augmenting CbbR-dependent control and optimizing expression of specific cbb operons. In addition to interactions with small effector metabolites and other regulator proteins, CbbR proteins may be selected that are constitutively active and, in some instances, elevate the level of cbb expression relative to wild-type CbbR. Optimizing CbbR-dependent control is an important consideration for potentially using microbes to convert CO 2 to useful bioproducts.

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“…It is also possible to study DNA and polysaccharides using packages in the HYDRO family Benitez et al 2015). We have followed such practice for proteins, RNA, DNA, and their complexes (Ariyo et al 2015;Deo et al 2014Deo et al , 2015Dzananovic et al 2013Dzananovic et al , 2014Meier et al 2013;Patel et al 2010Patel et al , 2011Patel et al , 2012Patel et al , 2014Vadlamani et al 2015).…”

Section: Molecular Weight Determinationmentioning

confidence: 99%

Dynamic light scattering: a practical guide and applications in biomedical sciences

2016

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Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein-protein or protein-nucleic acid preparations, as well as to study protein-small molecule interactions. Further, we provide examples of DLS's application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii.

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The β-Lactamase Gene Regulator AmpR Is a Tetramer That Recognizes and Binds the d-Ala-d-Ala Motif of Its Repressor UDP-N-acetylmuramic Acid (MurNAc)-pentapeptide

Cited by 67 publications

References 66 publications

Pseudomonas Aeruginosa : Targeting Cell-Wall Metabolism for New Antibacterial Discovery and Development

Pseudomonas Aeruginosa : Targeting Cell-Wall Metabolism for New Antibacterial Discovery and Development

CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation

Dynamic light scattering: a practical guide and applications in biomedical sciences

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