The α5 GABAA receptor subunit confers resistance to isoflurane inhibition

Orser, Beverley A.; Caraiscos, Valerie B.; You-Ten, Eric; Cheng, Victor; Newell, J. Glen; MacDonald, John F.

doi:10.1016/j.ics.2005.06.085

Cited by 1 publication

(1 citation statement)

References 19 publications

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“…Propofol's selectivity was demonstrated by introducing mutations in the β subunits, which eliminated its potentiation of GABA A receptors [87,94]. The involvement of β subunits has also been shown for the potentiating action of pentobarbital on these receptors [96][97][98]. Collectively, these findings suggest that preferential binding by the anesthetic agents under investigation may play a role in the varying potentiation effects of these anesthetics through the GABA A receptor.…”

Section: Discussionmentioning

confidence: 81%

Effect of anesthesia on motor responses evoked by spinal neural prostheses during intraoperative procedures

et al. 2019

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Objective. The overall goal of this study was to investigate the effects of various anesthetic protocols on the intraoperative responses to intraspinal microstimulation (ISMS). ISMS is a neuroprosthetic approach that targets the motor networks in the ventral horns of the spinal cord to restore function after spinal cord injury. In preclinical studies, ISMS in the lumbosacral enlargement produced standing and walking by activating networks controlling the hindlimb muscles. ISMS implants are placed surgically under anesthesia, and refinements in placement are made based on the evoked responses. Anesthesia can have a significant effect on the responses evoked by spinal neuroprostheses; therefore, in preparation for clinical testing of ISMS, we compared the evoked responses under a common clinical neurosurgical anesthetic protocol with those evoked under protocols commonly used in preclinical studies. Approach. Experiments were conducted in seven pigs. An ISMS microelectrode array was implanted in the lumbar enlargement and responses to ISMS were measured under three anesthetic protocols: (1) isoflurane, an agent used pre-clinically and clinically, (2) total intravenous anesthesia (TIVA) with propofol as the main agent commonly used in clinical neurosurgical procedures, (3) TIVA with sodium pentobarbital, an anesthetic agent used mostly preclinically. Responses to ISMS were evaluated based on stimulation thresholds, movement kinematics, and joint torques. Motor evoked potentials (MEP) and plasma concentrations of propofol were also measured. Main results. ISMS under propofol anesthesia produced large and functional responses that were not statistically different from those produced under pentobarbital anesthesia. Isoflurane, however, significantly suppressed the ISMS-evoked responses. Significance. This study demonstrated that the choice of anesthesia is critical for intraoperative assessments of motor responses evoked by spinal neuroprostheses. Propofol and pentobarbital anesthesia did not overly suppress the effects of ISMS; therefore, propofol is expected to be a suitable anesthetic agent for clinical intraoperative testing of an intraspinal neuroprosthetic system.

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