The zygotic segmentation mutant tailless alters the blastoderm fate map of the Drosophila embryo

Mahoney, Paul A.; Lengyel, Judith A.

doi:10.1016/0012-1606(87)90310-1

Cited by 56 publications

(23 citation statements)

References 15 publications

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“…For example, Tc'otd-1 and Tc'hunchback are expressed in and are required for the formation of head and thoracic segments in both Drosophila and Tribolium (Finkelstein et al, 1990;Hulskamp and Tautz, 1991;Li et al, 1996;Royet and Finkelstein, 1995;Schroder, 2003;Wolff et al, 1995). In addition, Tc'tailless is expressed in similar head regions as in Drosophila (Mahoney and Lengyel, 1987;Schroder et al, 2000;Weigel et al, 1990), and we have shown in this paper that the anterior stripe of Tc'giant covers the maxillary segment in both beetle and fly. Anterior conservation is also observed in more basal insect taxa: In the grasshopper Schistocerca, hunchback is also expressed in gnathal and thoracic segments (Patel et al, 2001).…”

Section: Gap Genes In Long and Short Germ Embryosmentioning

confidence: 61%

Divergent segmentation mechanism in the short germ insectTriboliumrevealed bygiantexpression and function

2004

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Segmentation is well understood in Drosophila, where all segments are determined at the blastoderm stage. In the flour beetle Tribolium castaneum, as in most insects, the posterior segments are added at later stages from a posteriorly located growth zone, suggesting that formation of these segments may rely on a different mechanism. Nevertheless, the expression and function of many segmentation genes seem conserved between Tribolium and Drosophila. We have cloned the Tribolium ortholog of the abdominal gap gene giant. As in Drosophila, Tribolium giant is expressed in two primary domains, one each in the head and trunk. Although the position of the anterior domain is conserved, the posterior domain is located at least four segments anterior to that of Drosophila. Knockdown phenotypes generated with morpholino oligonucleotides, as well as embryonic and parental RNA interference, indicate that giant is required for segment formation and identity also in Tribolium. In giant-depleted embryos,the maxillary and labial segment primordia are normally formed but assume thoracic identity. The segmentation process is disrupted only in postgnathal metamers. Unlike Drosophila, segmentation defects are not restricted to a limited domain but extend to all thoracic and abdominal segments, many of which are specified long after giant expression has ceased. These data show that giant in Tribolium does not function as in Drosophila, and suggest that posterior gap genes underwent major regulatory and functional changes during the evolution from short to long germ embryogenesis.

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Section: Gap Genes In Long and Short Germ Embryosmentioning

confidence: 61%

Divergent segmentation mechanism in the short germ insectTriboliumrevealed bygiantexpression and function

2004

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“…In the most severely affected tll 1 embryos, the seventh stripe of ftz is lost. This altered ftz pattern is known to be the consequence of cell fate changes, partly owing to the posterior expansion of kni, when tll is mutated (Mahoney and Lengyel 1987 …”

Section: Atro and Tll Interact Genetically To Repress Kni Expression mentioning

confidence: 99%

Histone deacetylase-associating Atrophin proteins are nuclear receptor corepressors

Wang¹,

Rajan²,

Pitman³

et al. 2006

Genes Dev.

126

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Drosophila Tailless (Tll) is an orphan nuclear receptor involved in embryonic segmentation and neurogenesis. Although Tll exerts potent transcriptional repressive effects, the underlying molecular mechanisms have not been determined. Using the established regulation of knirps by tll as a paradigm, we report that repression of knirps by Tll involves Atrophin, which is related to vertebrate Atrophin

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“…Four-to twelve-hour embryos were fixed as described previously (Ingham et al 1985) and treated with proteinase K prior to hybridization. Hybridization of the digoxigenin-labeled probe to embryos was based on the protocol of Mahoney and Lengyel (1987), whereas incubation of embryos with an anti-digoxigenin antibody conjugated to alkaline phosphatase and signal development were performed based on protocols of Boehringer-Marmheim.…”

Section: In Situ Hybridizations To Rnamentioning

confidence: 99%

Functional analysis of NTF-1, a developmentally regulated Drosophila transcription factor that binds neuronal cis elements.

Dynlacht¹,

Attardi²,

Admon³

et al. 1989

Genes Dev.

135

142

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In an effort to characterize sequence-specific transcription factors that regulate gene expression during Drosophila development, we identified and purified a novel DNA-binding activity (NTF-1). The purified protein consists of several polypeptides that bind selectively to a functionally important cis-control element of the Ultrabitborax {Ubx) promoter and to the neurogenic elements of both the dopa decarboxylase {Ddc) and fushi tarazu {ftz) promoter/enhancer regions. Purified NTF-1 activates transcription in vitro in a binding sitedependent manner through upstream sequences of the Ubx promoter. A cDNA clone encoding the open reading frame of NTF-1 was isolated, and the deduced primary amino acid sequence of NTF-1 includes a glutamine-rich region reminiscent of the transcriptional activation domains found in Spl but no recognizable DNA-binding domain. NTF-1 expression is temporally regulated during embryonic development. In addition, in situ hybridization experiments revealed that NTF-1 is transcribed in a spatially restricted pattern in the embryo, with the highest level of expression observed in the epidermis and a subset of cells in the CNS. Expression of the NTF-1 cDNA in mammalian cells yields a protein that displays DNA-binding and transcriptional activities indistinguishable from that of the collection of proteins isolated from Drosophila embryos. These findings suggest that NTF-1 is a member of a family of developmentally regulated transcription factors that may be involved in directing the expression of genes such as Ubx, Ddc, and ftz in neuronal cells.

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The zygotic segmentation mutant tailless alters the blastoderm fate map of the Drosophila embryo

Cited by 56 publications

References 15 publications

Divergent segmentation mechanism in the short germ insectTriboliumrevealed bygiantexpression and function

Divergent segmentation mechanism in the short germ insectTriboliumrevealed bygiantexpression and function

Histone deacetylase-associating Atrophin proteins are nuclear receptor corepressors

Functional analysis of NTF-1, a developmentally regulated Drosophila transcription factor that binds neuronal cis elements.

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