The zinc-finger protein CNBP is required for forebrain formation in the mouse

Chen, Wei; Liang, Yuqiong; Deng, Wenjie; Shimizu, Ken; Ashique, Amir M.; Li, En; Li, Yiping

doi:10.1242/dev.00349

Cited by 67 publications

(78 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Knockout experiments in mice have shown that CNBP is essential for forebrain formation (43). In conjunction with other studies, which have demonstrated an increase of c-myc promotor activity and cell proliferation upon overexpression of CNBP (44), it has been proposed that CNBP regulates forebrain formation through induction of c-Myc expression, which in turn stimulates cell proliferation and differentiation (43).…”

Section: Fliesmentioning

confidence: 97%

Identification of Mushroom body miniature, a zinc-finger protein implicated in brain development of Drosophila

Raabe

Clemens-Richter²,

Twardzik³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The mushroom bodies are bilaterally arranged structures in the protocerebrum of Drosophila and most other insect species. Mutants with altered mushroom body structure have been instrumental not only in establishing their role in distinct behavioral functions but also in identifying the molecular pathways that control mushroom body development. The mushroom body miniature 1 (mbm 1 ) mutation results in grossly reduced mushroom bodies and odor learning deficits in females. With a survey of genomic rescue constructs, we have pinpointed mbm 1 to a single transcription unit and identified a single nucleotide exchange in the 5 untranslated region of the corresponding transcript resulting in a reduced expression of the protein. A daptive behavior of animals and humans requires functional neuronal circuits in the brain. The genetic programs that control the generation of these circuits by providing an adequate number of neurons, establishing neuronal connectivity, and remodeling them during development and in response to external stimuli during adulthood are just beginning to emerge. The mushroom bodies (MBs), a prominent neuropil structure of the insect brain (1), have become an attractive model system to study many aspects of this intricate network. Functional studies have established a role of the MBs in olfactory learning and memory, controlling locomotor activity, performing visual context generalization, and decision making (2, 3). On the other hand, the structural organization and the development of the MBs have been described in great detail in refs. 4-10. In the adult fly Drosophila melanogaster, Ϸ2,500 intrinsic neurons (Kenyon cells) build up one MB. The Kenyon cell bodies are located in the dorsal cortex and extend their dendritic branches into the calyx, where prominent inputs from other brain regions are received. The Kenyon cell axons fasciculate in the peduncle and extend rostroventral, where most of them bifurcate to form a system of medially and dorsally projecting lobes. Each MB arises from a group of four apparently equipotent neuronal stem cells (neuroblasts), each of which generates in a sequential manner several types of Kenyon cells during larval and pupal stages. MB ␥ neurons are born before the mid-third-larval instar, then ␣Ј͞␤Ј neurons are born, and finally the ␣͞␤ neurons are added at pupal stages (6,8). The nomenclature of the Kenyon cells refers to the corresponding dorsally and medially projecting MB lobes in the adult fly (see Fig. 2). More recently, immunohistochemical studies have identified additional subtypes of Kenyon cells (9). Yet, the anatomical description disregards the structural plasticity of the adult MBs as a consequence of changes in living conditions and experience (11). Furthermore, Kenyon cell axons and dendrites undergo massive remodeling during metamorphosis to establish adult-specific branching patterns. The axons of the ␥ neurons, which bifurcate in a dorsal and a medial branch in the larvae, degenerate and regrow only in the medial direction (8,10,(12)(13)(14).What...

show abstract

Section: Fliesmentioning

confidence: 97%

Identification of Mushroom body miniature, a zinc-finger protein implicated in brain development of Drosophila

Raabe

Clemens-Richter²,

Twardzik³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…CNBP is highly conserved among human, mouse, rat, chicken, Xenopus species and fish [8]. The disruption of CNBP caused severe forebrain truncation and defective neural crest development [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding

Wei

Chang

et al. 2014

FEBS Letters

View full text Add to dashboard Cite

Edited by Michael IbbaKeywords: CNBP RNA binding Protein arginine methylation a b s t r a c t Cellular nucleic acid binding protein (CNBP) contains seven zinc finger (ZF) repeats and an arginine and glycine (RG) rich sequence between the first and the second ZF. CNBP interacts with protein arginine methyltransferase PRMT1. Full-length but not RG-deleted or mutated CNBP can be methylated. Treatment with a methylation inhibitor AdOx reduced CNBP methylation, but did not affect the concentrated nuclear localization of CNBP. Nevertheless, arginine methylation of CNBP appeared to interfere with its RNA binding activity. Our findings show that arginine methylation of CNBP in the RG motif did not change the subcellular localization, but regulated its RNA binding activity. Structured summary of protein interactions:PRMT1 binds to CNBP by pull down (View interaction) PRMT1 methylates CNBP by enzymatic study (View interaction) CNBP physically interacts with PRMT1 by anti tag coimmunoprecipitation (View interaction)

show abstract

“…During embryogenesis, the CNBP expression patterns have been analyzed in Bufo arenarum, Xenopus leavis, Danio rerio, mouse and chick [3,8,9,[11][12][13]. During early embryogenesis, CNBP mRNA could be detected in the The nucleotide sequence data reported in this paper have been submitted to the GenBank under accession number: CagCNBP (AY773183).…”

Section: Introductionmentioning

confidence: 99%

“…During early embryogenesis, CNBP mRNA could be detected in the The nucleotide sequence data reported in this paper have been submitted to the GenBank under accession number: CagCNBP (AY773183). unfertilized eggs and in the all embryonic cells in frog, zebrafish, cattle, chick and mouse [3,8,9,[11][12][13]. In mouse, during organogenesis, CNBP expresses predominantly in forebrain and midbrain, and plays important roles in cell proliferation and tissue patterning [12], and it is essential for the forebrain induction and specification [11].…”

Section: Introductionmentioning

confidence: 99%

“…unfertilized eggs and in the all embryonic cells in frog, zebrafish, cattle, chick and mouse [3,8,9,[11][12][13]. In mouse, during organogenesis, CNBP expresses predominantly in forebrain and midbrain, and plays important roles in cell proliferation and tissue patterning [12], and it is essential for the forebrain induction and specification [11]. In chick, CNBP acts as a regulator of the forebrain formation in rostral head development during organogenesis by regulating other rostral head transcription factors [13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp

2008

View full text Add to dashboard Cite

By differential screening, we cloned the Cag-CNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181-192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed.

show abstract

The zinc-finger protein CNBP is required for forebrain formation in the mouse

Cited by 67 publications

References 57 publications

Identification of Mushroom body miniature, a zinc-finger protein implicated in brain development of Drosophila

Identification of Mushroom body miniature, a zinc-finger protein implicated in brain development of Drosophila

Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding

Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp

Contact Info

Product

Resources

About