The zebrafish visual system transmits dimming information via multiple segregated pathways

Robles, Estuardo; Fields, Nicholas P.; Baier, Herwig

doi:10.1002/cne.24964

Cited by 13 publications

(14 citation statements)

References 45 publications

(82 reference statements)

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“…A dim-sensitive cluster (blue, Figure 3A ), responsive both to dims and full looms, contained the fewest ROIs, and these were principally spread across the habenulae, thalamus, tectum, and pallium, consistent with previous observations ( Cheng et al, 2017 ; Zhang et al, 2017 ; Chen et al, 2018 ; Heap L. A. L. et al, 2018 ; Robles et al, 2021 ). On average, these ROIs also had very weak responses to checkerboard stimuli.…”

Section: Resultssupporting

confidence: 88%

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Mancienne

Márquez-Legorreta

Wilde

et al. 2021

Front. Neural Circuits

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Animals from insects to humans perform visual escape behavior in response to looming stimuli, and these responses habituate if looms are presented repeatedly without consequence. While the basic visual processing and motor pathways involved in this behavior have been described, many of the nuances of predator perception and sensorimotor gating have not. Here, we have performed both behavioral analyses and brain-wide cellular-resolution calcium imaging in larval zebrafish while presenting them with visual loom stimuli or stimuli that selectively deliver either the movement or the dimming properties of full loom stimuli. Behaviorally, we find that, while responses to repeated loom stimuli habituate, no such habituation occurs when repeated movement stimuli (in the absence of luminance changes) are presented. Dim stimuli seldom elicit escape responses, and therefore cannot habituate. Neither repeated movement stimuli nor repeated dimming stimuli habituate the responses to subsequent full loom stimuli, suggesting that full looms are required for habituation. Our calcium imaging reveals that motion-sensitive neurons are abundant in the brain, that dim-sensitive neurons are present but more rare, and that neurons responsive to both stimuli (and to full loom stimuli) are concentrated in the tectum. Neurons selective to full loom stimuli (but not to movement or dimming) were not evident. Finally, we explored whether movement- or dim-sensitive neurons have characteristic response profiles during habituation to full looms. Such functional links between baseline responsiveness and habituation rate could suggest a specific role in the brain-wide habituation network, but no such relationships were found in our data. Overall, our results suggest that, while both movement- and dim-sensitive neurons contribute to predator escape behavior, neither plays a specific role in brain-wide visual habituation networks or in behavioral habituation.

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Section: Resultssupporting

confidence: 88%

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Mancienne

Márquez-Legorreta

Wilde

et al. 2021

Front. Neural Circuits

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“…We propose that the unique wiring geometry of the TL-PyrN circuit serves to degrade topographic precision to generate PyrNs with large visual RFs. TL receives visual input from tectum via TLPNs, visually responsive tectal neurons with small dendrites in SFGS that project to TL (Robles et al, 2020). Single cell labeling of TLPNs has confirmed that their axons form a coarse topographic projection to TL (DeMarco et al, 2019).…”

Section: Discussionmentioning

confidence: 95%

Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis

et al. 2021

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The torus longitudinalis (TL) is a midbrain structure unique to ray finned fish. Although previously implicated in orienting behaviors elicited by changes in ambient lighting, the role of TL in visual processing is not well-understood. TL is reciprocally connected to tectum and is the only known source of synaptic input to the stratum marginalis (SM) layer of tectal neuropil. Conversely, tectal pyramidal neurons (PyrNs) are the only identified tectal neuron population that forms a dendrite in SM. In this study we describe a zebrafish gal4 transgenic that labels TL neurons that project to SM. We demonstrate that the axonal TL projection to SM in zebrafish is glutamatergic. Consistent with these axons synapsing directly onto PyrNs, SM-targeted dendrites of PyrNs contain punctate enrichments of the glutamatergic post-synaptic marker protein PSD95. Sparse genetic labeling of individual TL axons and PyrN dendrites enabled quantitative morphometric analysis that revealed (1) large, sparsely branched TL axons in SM and (2) small, densely innervated PyrN dendrites in SM. Together this unique combination of morphologies support a wiring diagram in which TL inputs to PyrNs exhibit a high degree of convergence. We propose that this convergence functions to generate large, compound visual receptive fields in PyrNs. This quantitative anatomical data will instruct future functional studies aimed at identifying the precise contribution of TL-PyrN circuitry to visual behavior.

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“…In the case of larval zebrafish, some of this information is already encoded in retinal ganglion cells as they arrive in the tectal neuropil, including motion-and size-specific retinal ganglion cells that terminate in the superficial and intermediate layers of the neuropil 118,120 , and retinal ganglion cells encoding a drop in luminance (dim-specific retinal ganglion cells) in deeper layers 42,120 . These 'OFF' retinal ganglion cells present different sensitivity profiles and segregate this information in the neuropil, which in turn is received by OFF-sensitive tectal cells 123 . Additionally, dim-sensitive thalamic neurons project into the deep neuropil layers 93 .…”

Section: Reviewmentioning

confidence: 99%

The tectum/superior colliculus as the vertebrate solution for spatial sensory integration and action

et al. 2021

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The superior colliculus, or tectum in the case of non-mammalian vertebrates, is a part of the brain that registers events in the surrounding space, often through vision and hearing, but also through electrosensation, infrared detection, and other sensory modalities in diverse vertebrate lineages. This information is used to form maps of the surrounding space and the positions of different salient stimuli in relation to the individual. The sensory maps are arranged in layers with visual input in the uppermost layer, other senses in deeper positions, and a spatially aligned motor map in the deepest layer. Here, we will review the organization and intrinsic function of the tectum/superior colliculus and the information that is processed within tectal circuits. We will also discuss tectal/superior colliculus outputs that are conveyed directly to downstream motor circuits or via the thalamus to cortical areas to control various aspects of behavior. The tectum/superior colliculus is evolutionarily conserved among all vertebrates, but tailored to the sensory specialties of each lineage, and its roles have shifted with the emergence of the cerebral cortex in mammals. We will illustrate both the conserved and divergent properties of the tectum/superior colliculus through vertebrate evolution by comparing tectal processing in lampreys belonging to the oldest group of extant vertebrates, larval zebrafish, rodents, and other vertebrates including primates. ''The tectum/SC's intrinsic networks, and the computations that they perform'', to the sensory and sensorimotor integration ll

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The zebrafish visual system transmits dimming information via multiple segregated pathways

Cited by 13 publications

References 45 publications

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis

The tectum/superior colliculus as the vertebrate solution for spatial sensory integration and action

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