2011
DOI: 10.1152/advan.00099.2010
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The zebrafish brain in research and teaching: a simple in vivo and in vitro model for the study of spontaneous neural activity

Abstract: Recently, the zebrafish (Danio rerio) has been established as a key animal model in neuroscience. Behavioral, genetic, and immunohistochemical techniques have been used to describe the connectivity of diverse neural circuits. However, few studies have used zebrafish to understand the function of cerebral structures or to study neural circuits. Information about the techniques used to obtain a workable preparation is not readily available. Here, we describe a complete protocol for obtaining in vitro and in vivo… Show more

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Cited by 26 publications
(19 citation statements)
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“…Previous studies by others demonstrated similar increase of apoptosis following MSG exposure. Chronic accumulation of MSG induced pyramidal neural loss in cortical organotypic culture (Young et al, 2007) and retinal ganglion cell death (Vorwerk et al, 2000). Twenty hours exposure increased cytotoxicity of 1mM or 10mM glutamate on differentiating N1E-115 neuronal cells; morphological changes observed as neuritis retraction and cell shrinkage leading to apoptosis (Schelman et al, 2004).…”
Section: Msg Increased Circle Swimming Events and Decreased Locomotormentioning
confidence: 99%
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“…Previous studies by others demonstrated similar increase of apoptosis following MSG exposure. Chronic accumulation of MSG induced pyramidal neural loss in cortical organotypic culture (Young et al, 2007) and retinal ganglion cell death (Vorwerk et al, 2000). Twenty hours exposure increased cytotoxicity of 1mM or 10mM glutamate on differentiating N1E-115 neuronal cells; morphological changes observed as neuritis retraction and cell shrinkage leading to apoptosis (Schelman et al, 2004).…”
Section: Msg Increased Circle Swimming Events and Decreased Locomotormentioning
confidence: 99%
“…Brain tissue from 6 larva from each group were collected using glass micro-capillaries at 7 days after fertilization (Vargas et al, 2011). Brain tissue was homogenized for RNA isolation according to TRI reagent® (SigmaAldrich).…”
Section: Bcl-2 and Baxmentioning
confidence: 99%
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“…These experiments have largely been performed to detail physiological responses of various sensory systems [2][3][4][5] . Until recently, intact brain preparations have only been available for performing electrophysiology with zebrafish larva 3,6,7 , where respiration and oxygen diffusion can occur through the skin. Our preparation allows the native neurological activity of an adult zebrafish to be measured while the animal remains fully conscious and aware of its surroundings.…”
Section: Introductionmentioning
confidence: 99%
“…The use of zebrafish in cognitive and neurobiological aging research increased with the accumulation of knowledge about the neuroanatomy of the zebrafish brain. The standard neuroanatomical, neuropharmacological and immunohistochemical techniques have been applied to characterize the organization of the zebrafish brain [25,26]. These studies demonstrated similarities of zebrafish sensory and motor systems, and central nervous system circuits with other vertebrates.…”
mentioning
confidence: 99%