The Yfe system of <i>Yersinia pestis</i> transports iron and manganese and is required for full virulence of plague

Bearden, Scott W.; Perry, Robert D.

doi:10.1046/j.1365-2958.1999.01360.x

Cited by 242 publications

(308 citation statements)

References 62 publications

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“…This plasmid was electroporated into Y. pestis KIM6 and transformants were selected on TBA plates containing streptomycin (50 mg ml 21 ). As previously described (Bearden & Perry, 1999), cells grown overnight in Heart Infusion broth (Difco) without antibiotic selection were used to select sucrose-resistant isolates that had completed the allelic exchange. Strains in which the wild-type gene was replaced by the deleted version were identified by PCR using primers Iut-1 and Iut-2.…”

Section: Methodsmentioning

confidence: 99%

“…Feo appears to transport ferrous iron in a number of organisms, including Y. pestis (Perry et al, 2007). The Yfe, Yfu and Yiu systems are all ABC transporters that were originally identified by their ability to enhance the growth of an Escherichia coli enterobactinbiosynthetic mutant under iron-deficient conditions and were subsequently shown to function in Y. pestis as well (Bearden & Perry, 1999;Bearden et al, 1998;Gong et al, 2001;Hantke, 2004;Kirillina et al, 2006;Perry, 2004;Perry & Fetherston, 2004). …”

Section: Introductionmentioning

confidence: 99%

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Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

et al. 2007

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Yersinia pestis genomes contain genes homologous to the aerobactin receptor (iutA) and biosynthetic genes (iucABCD) as well as the ferric hydroxamate uptake system (fhuCDB) of Escherichia coli. However, iucA is disrupted by a frameshift mutation. An E. coli strain carrying the cloned Y. pestis aerobactin region was unable to produce aerobactin, but could use the siderophore as an iron source. Repair of the frameshift mutation in iucA did not allow aerobactin production in E. coli or Y. pestis. In contrast, a Y. pestis strain with a plasmid encoding the iucABCD-iutA genes from Shigella flexneri or pColV-K30 did produce and secrete the siderophore. In addition, Yersinia pseudotuberculosis PB1, which encodes the iucABCD-iutA locus without the Y. pestis-specific frameshift mutation, also failed to produce aerobactin. The Y. pestis fhuCDB operon, encoding an ABC transporter for a range of hydroxamate siderophores, was able to complement a strain of E. coli with a transposon insertion in fhuC, allowing utilization of aerobactin and ferrichrome. Y. pestis KIM6, a strain deficient in the production of the siderophore yersiniabactin, was able to use both the ferrichrome and the aerobactin siderophores as a source of iron. Mutations in iutA or the fhu operon abolished the ability of KIM6 to use aerobactin. Mutations in the fhu operon, but not in iutA, affected the ability of KIM6 to use ferrichrome. This demonstrates that Y. pestis uses both ferrichrome and aerobactin, but has lost the ability to synthesize aerobactin.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

et al. 2007

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“…PsaA/ScaA homologues have been identified in at least nine additional species of Streptococcus and also in bacteria belonging to other genera (for a review see Claverys, 2001). Mn 2+ uptake by ABC transporters has been experimentally demonstrated in Salmonella enterica serovar Typhimurium (Kehres et al, 2002;Kehres & Maguire, 2003) and specificity for both iron and manganese was shown for Yersinia pestis (Bearden & Perry, 1999), S. pyogenes (Janulczyk et al, 2003) and Streptococcus mutans (Paik et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Genome-based in silico detection of putative manganese transport systems in Lactobacillus plantarum and their genetic analysis

et al. 2005

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Manganese serves an important function in Lactobacillus plantarum in protection against oxidative stress and this bacterium can accumulate Mn 2+ up to millimolar levels intracellularly.Although the physiological role of Mn 2+ and the uptake of this metal ion have been well documented, the only uptake system described so far for this bacterium is the Mn 2+ -and Cd 2+-specific P-type ATPase (MntA). Recently, the genome of L. plantarum WCFS1 has been sequenced allowing in silico detection of genes potentially encoding Mn 2+ transport systems, using established microbial Mn 2+ transporters as the query sequence. This genome analysis revealed that L. plantarum WCFS1 encodes, besides the previously described mntA gene, an ABC transport system (mtsCBA) and three genes encoding Nramp transporters (mntH1, mntH2 and mntH3).

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“…Opsonising antibodies (Th1) directed against iron uptake ABC transporters of Streptococcus pneumoniae were shown to increase survival of mice, after exposure to a lethal dose of bacteria, from 0 to 80% via blockage of transporter function [33]. This is corroborated by the finding that Yersinia pestis mutants, deficient in the metal uptake ABC transporter locus, showed abridged virulence [34]. These results were obtained in Gram However, a significantly raised SI value was only detected in a few of the animals per group at a specific time point, and even then the values were in most cases only marginally higher than 2, nonetheless this does confirm that expression of the ORFs had taken place.…”

Section: Discussionmentioning

confidence: 90%

Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames

Pretorius

Collins

Steyn

et al. 2007

Vaccine

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The Yfe system of Yersinia pestis transports iron and manganese and is required for full virulence of plague

Cited by 242 publications

References 62 publications

Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

Genome-based in silico detection of putative manganese transport systems in Lactobacillus plantarum and their genetic analysis

Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames

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