The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis.

Schönbächler, M.; Horváth, Anton; Fassler, Jan S.; Riezman, Howard

doi:10.1002/j.1460-2075.1995.tb07152.x

Cited by 67 publications

(63 citation statements)

References 59 publications

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“…Intracellular protein processing was monitored by pulse-chase analysis with [ 35 S]methionine (Amersham Pharmacia Biotech), using anti-CPY and anti-Gas1p antibodies in immunoprecipitation reactions as described (47,48). Autoradiography was performed with a fluorescence enhancer.…”

Section: Methodsmentioning

confidence: 99%

Aut7p, a Soluble Autophagic Factor, Participates in Multiple Membrane Trafficking Processes

Legesse‐Miller

Sagiv

Glozman

et al. 2000

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Aut7p, a Soluble Autophagic Factor, Participates in Multiple Membrane Trafficking Processes

Legesse‐Miller

Sagiv

Glozman

et al. 2000

Journal of Biological Chemistry

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“…The GlcNAc transfer reaction is unexpectedly complex in that many proteins are involved. The mammalian proteins and their yeast orthologs are PIG-A/Gpi3p, PIG-C/Gpi2p, PIG-H/Gpi15p, PIG-P/Gpi19p, PIG-Q(hGpi1p)/Gpi1p, and PIG-Y/Eri1p (50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65). In mammalian cells, a seventh protein, Dpm2p, a noncatalytic subunit of Dol-P-Man synthase, physically associates with the PIG-A, -C, and -Q proteins and enhances GlcNAc-PI synthetic activity (58).…”

Section: Biosynthesis Of the Gpi Precursormentioning

confidence: 99%

Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids

Orlean

Menon

2007

Journal of Lipid Research

359

293

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Glycosylphosphatidylinositol (GPI) anchoring of cell surface proteins is the most complex and metabolically expensive of the lipid posttranslational modifications described to date. The GPI anchor is synthesized via a membrane-bound multistep pathway in the endoplasmic reticulum (ER) requiring .20 gene products. The pathway is initiated on the cytoplasmic side of the ER and completed in the ER lumen, necessitating flipping of a glycolipid intermediate across the membrane. The completed GPI anchor is attached to proteins that have been translocated across the ER membrane and that display a GPI signal anchor sequence at the C terminus. GPI proteins transit the secretory pathway to the cell surface; in yeast, many become covalently attached to the cell wall. Genes encoding proteins involved in all but one of the predicted steps in the assembly of the GPI precursor glycolipid and its transfer to protein in mammals and yeast have now been identified. Most of these genes encode polytopic membrane proteins, some of which are organized in complexes. The steps in GPI assembly, and the enzymes that carry them out, are highly conserved. GPI biosynthesis is essential for viability in yeast and for embryonic development in mammals. In this review, we describe the biosynthesis of mammalian and yeast GPIs, their transfer to protein, and their subsequent processing.-Orlean, P., and A. K. Menon. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids.

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“…GPI-anchor synthesis is essential in S. cerevisiae because mutants blocked in synthesis are not viable (124)(125)(126)(127). One essential yeast gene, SPT14, is necessary for the first step in GPI-anchor synthesis (125,126).…”

Section: Phytoceramide Replaces Diacylglycerol In Many Gpi Anchorsmentioning

confidence: 99%

SPHINGOLIPID FUNCTIONS INSACCHAROMYCES CEREVISIAE: Comparison to Mammals

Dickson

1998

Annu. Rev. Biochem.

249

206

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Many roles for sphingolipids have been identified in mammals. Available data suggest that sphingolipids and their intermediates also have diverse roles in Saccharomyces cerevisiae. These roles include signal transduction during the heat stress response, regulation of calcium homeostasis or components in calciummediated signaling pathways, regulation of the cell cycle, and functions as components in trafficking of secretory vesicles from the endoplasmic reticulum to the Golgi apparatus and as the lipid moiety in many glycosylphosphatidylinositolanchored proteins. S. cerevisiae is likely to be the first organism in which all genes involved in sphingolipid metabolism are identified. This information will provide an unprecedented opportunity to determine, for the first time in any organism, how sphingolipid synthesis is regulated. Through the use of both genetic and biochemical techniques, the identification of the complete array of processes regulated by sphingolipid signals is likely to be possible, as is the quantification of the physiological contribution of each. CONTENTS

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The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis.

Cited by 67 publications

References 59 publications

Aut7p, a Soluble Autophagic Factor, Participates in Multiple Membrane Trafficking Processes

Aut7p, a Soluble Autophagic Factor, Participates in Multiple Membrane Trafficking Processes

Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids

SPHINGOLIPID FUNCTIONS INSACCHAROMYCES CEREVISIAE: Comparison to Mammals

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