The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction

Liu, Guo-Song; Li, Tian; Zhou, Wei; Jiang, Min; Tao, Xinyi; Liu, Min; Zhao, Ming; Ren, Yuhong; Gao, Bei; Wang, Fengqing; Wei, Dongzhi

doi:10.1016/j.ymben.2019.11.001

Cited by 158 publications

(154 citation statements)

References 58 publications

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“…These challenges have brought recent attention to Yarrowia as a production host for plantderived terpenes due to its capacity to accumulate lipophilic compounds and the potential to utilize technology developed for S. cerevisiae in this new host [45,46]. A recent pivotal study harnessed peroxisomes to produce squalene at an unprecedented titer through dual cytoplasmic-peroxisomal engineering [47]. This study indicates that peroxisomes can function analogously to trichomes due to their pathway compartmentalization.…”

Section: Enhancing Product Accumulation Capacity Through Host Engineementioning

confidence: 93%

New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids

Belcher

Mahinthakumar

Keasling

2020

Current Opinion in Biotechnology

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Section: Enhancing Product Accumulation Capacity Through Host Engineementioning

confidence: 93%

New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids

Belcher

Mahinthakumar

Keasling

2020

Current Opinion in Biotechnology

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“…The production of squalene was increased by 71-fold, with the titer of 634 mg/L in shake flask fermentation ( Figure 1C). Liu et al (2020) compartmentalized yeast peroxisome as a subcellular factory for squalene biosynthesis. Hybridization of the cytoplasm-and peroxisome-engineered strains was constructed, and squalene with a titer of 11.0 g/L was reached in two-stage fed-batch fermentation (Figure 1D).…”

Section: Subcellular Engineering and Cell Free Systemmentioning

confidence: 99%

Production of Terpenoids by Synthetic Biology Approaches

Zhang

Hong

2020

Front. Bioeng. Biotechnol.

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Terpenoids are a large family of natural products with remarkable diverse biological functions, and have a wide range of applications as pharmaceuticals, flavors, pigments, and biofuels. Synthetic biology is presenting possibilities for sustainable and efficient production of high value-added terpenoids in engineered microbial cell factories, using Escherichia coli and Saccharomyces cerevisiae which are identified as well-known industrial workhorses. They also provide a promising alternative to produce non-native terpenes on account of available genetic tools in metabolic engineering and genome editing. In this review, we summarize the recent development in terpenoids production by synthetic biology approaches.

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“…Peroxisomes, ubiquitous eukaryotic organelles with a single bilayer membrane, have already proven to be suitable compartments for the production of polyhydroxyalkanoates used for biodegradable plastics as well as for the production of fatty alcohols, alkanes, and olefins used as drop-in fuels (Poirier et al, 2001;DeLoache et al, 2016;Zhou et al, 2016). Recently, peroxisomes were successfully used as compartments for the production of the triterpene squalene (Liu et al, 2020). Here, we tested their suitability for monoterpenoid production.…”

Section: Introductionmentioning

confidence: 99%

Production of the Fragrance Geraniol in Peroxisomes of a Product-Tolerant Baker’s Yeast

Gerke

Frauendorf

Schneider

et al. 2020

Front. Bioeng. Biotechnol.

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Monoterpenoids, such as the plant metabolite geraniol, are of high industrial relevance since they are important fragrance materials for perfumes, cosmetics, and household products. Chemical synthesis or extraction from plant material for industry purposes are complex, environmentally harmful or expensive and depend on seasonal variations. Heterologous microbial production offers a cost-efficient and sustainable alternative but suffers from low metabolic flux of the precursors and toxicity of the monoterpenoid to the cells. In this study, we evaluated two approaches to counteract both issues by compartmentalizing the biosynthetic enzymes for geraniol to the peroxisomes of Saccharomyces cerevisiae as production sites and by improving the geraniol tolerance of the yeast cells. The combination of both approaches led to an 80% increase in the geraniol titers. In the future, the inclusion of product tolerance and peroxisomal compartmentalization into the general chassis engineering toolbox for monoterpenoids or other host-damaging, industrially relevant metabolites may lead to an efficient, low-cost, and eco-friendly microbial production for industrial purposes.

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The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction

Cited by 158 publications

References 58 publications

New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids

New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids

Production of Terpenoids by Synthetic Biology Approaches

Production of the Fragrance Geraniol in Peroxisomes of a Product-Tolerant Baker’s Yeast

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