The Yeast Genome Directory

Mutations inRAD3, MSH2, andRAD52 affect the rate of gene amplification in the yeastSaccharomyces cerevisiae

“…The ends of the yeast genomic DNA insert in p724 were sequenced using Sanger dideoxy sequencing on a Perkin-Elmer model 373 or 377 automated sequencer (Foster City, CA) at the University of Cincinnati Molecular Biology Core. DNA sequences of 200 base pairs (bp) at the each end of the yeast genomic DNA insert in clone p724 were used to search the Saccharomyces cerevisiae genome database [Goffeau et al, 1997;http://genome-www.stanford.edu/Saccharomyces/]. This analysis showed that the DNA repair helicase gene RAD3 was located on the plasmid insert.…”

“…Two independent clones were identified, p724 and p931, which restored wild-type UV resistance to B9-40 cells. The DNA sequences at each end of the yeast genomic DNA insert in clone p724 were used to search the Saccharomyces genome database [Goffeau et al, 1997;http://genome-www.stanford.edu/Saccharomyces/]. This analysis showed that the yeast genomic DNA in p724 spanned base pairs 521,526 to 530,802 of chromosome V. This region contains three full-length open reading frames (ORF): a hypothetical ORF (YER169W), an adenylate kinase gene (ADK2), and the DNA repair helicase gene RAD3.…”

“…Only a single-base-pair difference from the published sequence at the Saccharomyces genome database [Goffeau et al, 1997; http://genome-www.stanford.edu/Saccharomyces/] was detected, a T 3 A transversion at base pair 1388. This predicts a change in the Rad3 protein at amino acid 463, from an uncharged isoleucine (ATA) to a charged lysine (AAA).…”

“…In rDNA amplification in Tetrahymena, recombination occurs between two identical 42-bp inverted repeats. Such large, perfect inverted repeats are not found in the yeast genome adjacent to ADH4 [Goffeau et al, 1997; http://genome-www.stanford.edu/Saccharomyces/]. However, several 8-to 10-bp inverted repeats are located between ADH4 and the centromere in a region where the novel joints on several linear palindromes were mapped by restriction analysis .…”

Relationships Between Transcriptional and Translational Control of Gene Expression in Saccharomyces cerevisiae: A Multiple Regression Analysis

“…The application of a recently devised technique, the serial analysis of gene expression (SAGE) of Velculescu and co-workers (1995), to S. cerevisiae, the only eukaryotic organism for which the entire genome has been characterized at the nucleotide level (Goffeau et al 1997), has allowed for the quantitative analysis of a large number of transcripts (Velculescu et al 1997). As a consequence, the knowledge of the absolute amount of transcript for individual genes, measured as the number of mRNA copies per cell, allows studying the pattern of codon usage also from the perspective of transcription.…”