The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel

Maciaszczyk-Dziubińska, Ewa; Migdal, Iwona; Migocka, Magdalena; Bocer, Tomasz; Wysocki, Robert

doi:10.1016/j.febslet.2009.12.027

Cited by 57 publications

(47 citation statements)

References 31 publications

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“…For copper uptake, the liquid SC-U/Glu yeast culture (OD600 ϳ 0.5) was supplied with 100 M CuCl 2 and subsampled after 1, 2, 4, and 8 h of growth in the presence of metal excess. The intracellular copper content was determined essentially as described earlier (24,25) using the Atomic Absorption Spectrophotometer 3300 (PerkinElmer Life Sciences).…”

Section: Methodsmentioning

confidence: 99%

Functional and Biochemical Characterization of Cucumber Genes Encoding Two Copper ATPases CsHMA5.1 and CsHMA5.2

Migocka¹,

Posyniak²,

Maciaszczyk-Dziubińska³

et al. 2015

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Functional and Biochemical Characterization of Cucumber Genes Encoding Two Copper ATPases CsHMA5.1 and CsHMA5.2

Migocka¹,

Posyniak²,

Maciaszczyk-Dziubińska³

et al. 2015

Journal of Biological Chemistry

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“…Aquaporins mediate non specific uptake of arsenite and mutants in the corresponding genes are usually arsenite resistant [7], [8], [9], [10], [61]. The only gene coding for this protein in Synechocystis ( aqpZ; slr2057 ) was repressed after both arsenate and arsenite (10 fold in arsenate and 6 fold in arsenite; Table S1).…”

Section: Resultsmentioning

confidence: 99%

Genomic Responses to Arsenic in the Cyanobacterium Synechocystis sp. PCC 6803

2014

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Arsenic is a ubiquitous contaminant and a toxic metalloid which presents two main redox states in nature: arsenite [AsIII] and arsenate [AsV]. Arsenic resistance in Synechocystis sp. strain PCC 6803 is mediated by the arsBHC operon and two additional arsenate reductases encoded by the arsI1 and arsI2 genes. Here we describe the genome-wide responses to the presence of arsenate and arsenite in wild type and mutants in the arsenic resistance system. Both forms of arsenic produced similar responses in the wild type strain, including induction of several stress related genes and repression of energy generation processes. These responses were transient in the wild type strain but maintained in time in an arsB mutant strain, which lacks the arsenite transporter. In contrast, the responses observed in a strain lacking all arsenate reductases were somewhat different and included lower induction of genes involved in metal homeostasis and Fe-S cluster biogenesis, suggesting that these two processes are targeted by arsenite in the wild type strain. Finally, analysis of the arsR mutant strain revealed that ArsR seems to only control 5 genes in the genome. Furthermore, the arsR mutant strain exhibited hypersentivity to nickel, copper and cadmium and this phenotype was suppressed by mutation in arsB but not in arsC gene suggesting that overexpression of arsB is detrimental in the presence of these metals in the media.

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“…The A. niger genomic sequences were obtained from the Central Aspergillus Data Repository (CADRE) (www.cadre-genomes.org.uk/Aspergillus_niger/). The putative arsenic homeostasis genes in Aspergillus niger were found by comparing gene and protein sequences with those of S. cerevisiae (12,35,39,49,61,62,66) using nucleotide-BLAST and protein-BLAST from the National Center for Biotechnology Information (http://blast.ncbi.nlm.nih .gov/Blast.cgi).…”

Section: Methodsmentioning

confidence: 99%

Role of Aspergillus niger acrA in Arsenic Resistance and Its Use as the Basis for an Arsenic Biosensor

Choe

Gravelat

Abdallah

et al. 2012

Appl Environ Microbiol

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ABSTRACTArsenic contamination of groundwater sources is a major issue worldwide, since exposure to high levels of arsenic has been linked to a variety of health problems. Effective methods of detection are thus greatly needed as preventive measures. In an effort to develop a fungal biosensor for arsenic, we first identified seven putative arsenic metabolism and transport genes inAspergillus niger, a widely used industrial organism that is generally regarded as safe (GRAS). Among the genes tested for RNA expression in response to arsenate,acrA, encoding a putative plasma membrane arsenite efflux pump, displayed an over 200-fold increase in gene expression in response to arsenate. We characterized the function of thisA. nigerprotein in arsenic efflux by gene knockout and confirmed that AcrA was located at the cell membrane using an enhanced green fluorescent protein (eGFP) fusion construct. Based on our observations, we developed a putative biosensor strain containing a construct of the native promoter ofacrAfused withegfp. We analyzed the fluorescence of this biosensor strain in the presence of arsenic using confocal microscopy and spectrofluorimetry. The biosensor strain reliably detected both arsenite and arsenate in the range of 1.8 to 180 μg/liter, which encompasses the threshold concentrations for drinking water set by the World Health Organization (10 and 50 μg/liter).

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The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel

Cited by 57 publications

References 31 publications

Functional and Biochemical Characterization of Cucumber Genes Encoding Two Copper ATPases CsHMA5.1 and CsHMA5.2

Functional and Biochemical Characterization of Cucumber Genes Encoding Two Copper ATPases CsHMA5.1 and CsHMA5.2

Genomic Responses to Arsenic in the Cyanobacterium Synechocystis sp. PCC 6803

Role of Aspergillus niger acrA in Arsenic Resistance and Its Use as the Basis for an Arsenic Biosensor

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