The yapA Encodes bZIP Transcription Factor Involved in Stress Tolerance in Pathogenic Fungus Talaromyces marneffei

Dankai, Wiyada; Pongpom, Monsicha; Youngchim, Sirida; Cooper, Chester R.; Vanittanakom, Nongnuch

doi:10.1371/journal.pone.0163778

Cited by 18 publications

(10 citation statements)

References 28 publications

(37 reference statements)

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“…Notably, the same phenotypes are observed in mutants affected in MoAP1 regulated genes MGG_01230 and MGG_15157, encoding succinic semialdehyde dehydrogenase MoSsadh and acetyltransferase MoAct, respectively (Guo et al, 2011), which however we did not detect as NapA-dependent. In the dimorphic fungus Talaromyces marneffei , yapA mutants are sensitive to H 2 O 2 and menadione, show decreased radial growth, produce conidiophores with fewer phialides and conidia, and conidia show decreased germination rates, while yeast cells fail to undergo binary fission (Dankai et al, 2016). Overall, the developmental roles of TFs long associated only with ROS detoxification support the role of ROS as developmental signals (Hansberg and Aguirre, 1990; Aguirre et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans

Mendoza-Martínez¹,

Lara-Rojas²,

Sánchez³

et al. 2017

Front. Microbiol.

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The redox-regulated transcription factors (TFs) of the bZIP AP1 family, such as yeast Yap1 and fission yeast Pap1, are activated by peroxiredoxin proteins (Prxs) to regulate the antioxidant response. Previously, Aspergillus nidulans mutants lacking the Yap1 ortholog NapA have been characterized as sensitive to H2O2 and menadione. Here we study NapA roles in relation to TFs SrrA and AtfA, also involved in oxidant detoxification, showing that these TFs play different roles in oxidative stress resistance, catalase gene regulation and development, during A. nidulans life cycle. We also uncover novel NapA roles in repression of sexual development, normal conidiation, conidial mRNA accumulation, and carbon utilization. The phenotypic characterization of ΔgpxA, ΔtpxA, and ΔtpxB single, double and triple peroxiredoxin mutants in wild type or ΔnapA backgrounds shows that none of these Prxs is required for NapA function in H2O2 and menadione resistance. However, these Prxs participate in a minor NapA-independent H2O2 resistance pathway and NapA and TpxA appear to regulate conidiation along the same route. Using transcriptomic analysis we show that during conidial development NapA-dependent gene expression pattern is different from canonical oxidative stress patterns. In the course of conidiation, NapA is required for regulation of at least 214 genes, including ethanol utilization genes alcR, alcA and aldA, and large sets of genes encoding proteins involved in transcriptional regulation, drug detoxification, carbohydrate utilization and secondary metabolism, comprising multiple oxidoreductases, membrane transporters and hydrolases. In agreement with this, ΔnapA mutants fail to grow or grow very poorly in ethanol, arabinose or fructose as sole carbon sources. Moreover, we show that NapA nuclear localization is induced not only by oxidative stress but also by growth in ethanol and by carbon starvation. Together with our previous work, these results show that SakA-AtfA, SrrA and NapA oxidative stress-sensing pathways regulate essential aspects of spore physiology (i.e., cell cycle arrest, dormancy, drug production and detoxification, and carbohydrate utilization).

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Section: Discussionmentioning

confidence: 99%

NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans

Mendoza-Martínez¹,

Lara-Rojas²,

Sánchez³

et al. 2017

Front. Microbiol.

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“…The same team later reported that the suppression of the yap1 orthologous gene led to increased OTA accumulation in Aspergillus ochraceus [ 124 ]. In T. marneffei , the mutant ∆ yapA , a yap1 orthologous gene, was found to be sensitive to oxidative chemicals such as H 2 O 2 or menadione and featured growth, germination, and conidiation delays [ 125 ]. For the genus Penicillium , the only works on orthologues Skn7 and Yap1 are mentioned above; their roles in the secondary metabolism of T. marneffei have not yet been investigated.…”

Section: Regulation Of Secondary Metabolismmentioning

confidence: 99%

Regulation of Secondary Metabolism in the Penicillium Genus

Assaf

Zetina-Serrano

Tahtah

et al. 2020

IJMS

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Penicillium, one of the most common fungi occurring in a diverse range of habitats, has a worldwide distribution and a large economic impact on human health. Hundreds of the species belonging to this genus cause disastrous decay in food crops and are able to produce a varied range of secondary metabolites, from which we can distinguish harmful mycotoxins. Some Penicillium species are considered to be important producers of patulin and ochratoxin A, two well-known mycotoxins. The production of these mycotoxins and other secondary metabolites is controlled and regulated by different mechanisms. The aim of this review is to highlight the different levels of regulation of secondary metabolites in the Penicillium genus.

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“…Deletion of the yapA was hypersensitive to oxidative and nitrosative stress. This mutant exhibited decreased survival inside a human macrophage cell line compared with the wild-type and the complemented strain [ 52 ]. Hence, yapA appears to have a role in the stress response of T. marneffei .…”

Section: The Regulation Of Fungal Response To Oxidative Stressmentioning

confidence: 99%

Adaptation to macrophage killing by Talaromyces marneffei

et al. 2017

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Talaromyces (Penicillium) marneffei is an important opportunistic fungal pathogen. It causes disseminated infection in immunocompromised patients especially in Southeast Asian countries. The pathogenicity of T. marneffei depends on the ability of the fungus to survive the killing process and replicate inside the macrophage. Major stresses inside the phagosome of macrophages are heat, oxidative substances and nutrient deprivation. The coping strategies of this pathogen with these stresses are under investigation. This paper summarizes factors relating to the stress responses that contribute to the intracellular survival of T. marneffei. These include molecules in the MAP signal transduction cascade, heat shock proteins, antioxidant enzymes and enzymes responsible in nutrient retrieval. There is speculation that the ability of T. marneffei to withstand these defenses plays an important role in its pathogenicity.

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The yapA Encodes bZIP Transcription Factor Involved in Stress Tolerance in Pathogenic Fungus Talaromyces marneffei

Cited by 18 publications

References 28 publications

NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans

NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans

Regulation of Secondary Metabolism in the Penicillium Genus

Adaptation to macrophage killing by Talaromyces marneffei

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