The YABBY Family Transcription Factor AaYABBY5 Directly Targets Cytochrome P450 Monooxygenase (CYP71AV1) and Double-Bond Reductase 2 (DBR2) Involved in Artemisinin Biosynthesis in Artemisia Annua

Kayani, Sadaf‐Ilyas; Shen, Qian; Ma, Yong; Fu, Xueqing; Xie, Lihui; Zhong, Yijun; Chen, Tiantian; Pan, Qin; Li, Ling; Rahman, Saeed Ur; Sun, Xiaofen

doi:10.3389/fpls.2019.01084

Cited by 33 publications

(35 citation statements)

References 54 publications

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“…The effector and reporter constructs were transformed into the yeast strain EGY48 following the manufacturer’s instruction (Clontech, http://www.clontech.com ). The transformants were firstly grown and selected on the SD/-Trp/-Ura medium, and the positive colonies were then transferred onto the SD/-Trp-Ura medium containg 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside for coloration (Kayani et al 2019 ). The primers used are listed in Supplementary Table S4.…”

Section: Methodsmentioning

confidence: 99%

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OsNAC109 regulates senescence, growth and development by altering the expression of senescence- and phytohormone-associated genes in rice

Shí

et al. 2021

Plant Mol Biol

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Key message We demonstrate that OsNAC109 regulates senescence, growth and development via binding to the cis-element CNTCSSNNSCAVG and altering the expression of multiple senescence- and hormone-associated genes in rice. Abstract The NAC family is one of the largest transcripton factor families in plants and plays an essential role in plant development, leaf senescence and responses to biotic/abiotic stresses through modulating the expression of numerous genes. Here, we isolated and characterized a novel yellow leaf 3 (yl3) mutant exhibiting arrested-growth, increased accumulation of reactive oxygen species (ROS), decreased level of soluble proteins, increased level of malondialdehyde (MDA), reduced activities of ROS scavenging enzymes, altered expression of photosynthesis and senescence/hormone-associated genes. The yellow leaf and arrested-growth trait was controlled by a single recessive gene located to chromosome 9. A single nucleotide substitution was detected in the mutant allele leading to premature termination of its coding protein. Genetic complementation could rescue the mutant phenotype while the YL3 knockout lines displayed similar phenotype to WT. YL3 was expressed in all tissues tested and predicted to encode a transcriptional factor OsNAC109 which localizes to the nucleus. It was confirmed that OsNAC109 could directly regulate the expression of OsNAP, OsNYC3, OsEATB, OsAMTR1, OsZFP185, OsMPS and OsGA2ox3 by targeting to the highly conserved cis-element CNTCSSNNSCAVG except OsSAMS1. Our results demonstrated that OsNAC109 is essential to rice leaf senescence, growth and development through regulating the expression of senescence- and phytohormone-associated genes in rice.

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Section: Methodsmentioning

confidence: 99%

“…com). The transformants were firstly grown and selected on the SD/-Trp/-Ura medium, and the positive colonies were then transferred onto the SD/-Trp-Ura medium containg 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside for coloration (Kayani et al 2019). The primers used are listed in Supplementary Table S4.…”

Section: Yeast One-hybrid Assaymentioning

confidence: 99%

OsNAC109 regulates senescence, growth and development by altering the expression of senescence- and phytohormone-associated genes in rice

Shí

et al. 2021

Plant Mol Biol

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“…Furthermore, the YABBY genes are associated with the laminar growth, as well as suppression of shoot apical meristem (SAM) pattern genes ( Sarojam et al, 2010 ). YABBY is a type of transcription factors that is specifically found in seed plants ( Kayani et al, 2019 ). The YABBY gene family is relatively small and has independent origins in the seed plant genealogy ( Floyd & Bowman, 2007 ; Liu et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and analysis of the YABBY gene family in Moso Bamboo (Phyllostachys edulis (Carrière) J. Houz)

Huang

et al. 2021

PeerJ

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Background The YABBY gene family is a family of small zinc finger transcription factors associated with plant morphogenesis, growth, and development. In particular, it is closely related to the development of polarity in the lateral organs of plants. Despite being studied extensively in many plant species, there is little information on genome-wide characterization of this gene family in Moso bamboo. Methods In the present study, we identified 16 PeYABBY genes, which were unequally distributed on 11 chromosomes, through genome-wide analysis of high-quality genome sequences of M oso bamboo by bioinformatics tools and biotechnological tools. Gene expression under hormone stress conditions was verified by quantitative real-time PCR (qRT-PCR) experiments. Results Based on peptide sequences and similarity of exon-intron structures, we classified the PeYABBY genes into four subfamilies. Analysis of putative cis-acting elements in promoters of these genes revealed that PeYABBYs contained a large number of hormone-responsive and stress-responsive elements. Expression analysis showed that they were expressed at a high level in Moso bamboo panicles, rhizomes, and leaves. Expression patterns of putative PeYABBY genes in different organs and hormone-treated were analyzed using RNA-seq data, results showed that some PeYABBY genes were responsive to gibberellin (GA) and abscisic acid (ABA), indicating that they may play an important role in plant hormone responses. Gene Ontology (GO) analyses of YABBY proteins indicated that they may be involved in many developmental processes, particularly high level of enrichment seen in plant leaf development. In summary, our results provide a comprehensive genome-wide study of the YABBY gene family in bamboos, which could be useful for further detailed studies of the function and evolution of the YABBY genes, and to provide a fundamental basis for the study of YABBY in Gramineae for resistance to stress and hormonal stress.

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“…In this study, shoot organogenesis induction positively affected the biosynthesis of phenylpropanoids, flavonoids, flavones, and flavonols ( Supplementary Material 1 and Table 3 ). Moreover, treated samples exhibited the upregulation of several transcription factors associated with the biosynthesis of secondary metabolites (i.e., bHLH, MYB, NAC, WRKY, and YABBY) ( Table 3 ) (Pal et al, 2018 ; Kayani et al, 2019 ; Xie et al, 2020 ). The biosynthetic pathways activated during shoot organogenesis induction of M. glaucescens indicate a strong potential for the production of bioactive compounds with a wide range of functions.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

et al. 2021

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Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the genetic regulation underlying its developmental processes were known. The present study generated de novo transcriptome data, describing in vitro shoot organogenesis induction in M. glaucescens. Total RNA was extracted from explants before (control) and after shoot organogenesis induction (treated). A total of 14,478 unigenes (average length, 520 bases) were obtained using Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) sequencing and transcriptome assembly. Filtering for differential expression yielded 2,058 unigenes. Pairwise comparison of treated vs. control genes revealed that 1,241 (60.3%) unigenes exhibited no significant change, 226 (11%) were downregulated, and 591 (28.7%) were upregulated. Based on database analysis, more transcription factor families and unigenes appeared to be upregulated in the treated samples than in controls. Expression of WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) and CALMODULIN (CaM) genes, both of which were upregulated in treated samples, was further validated by real-time quantitative PCR (RT-qPCR). Differences in gene expression patterns between control and treated samples indicate substantial changes in the primary and secondary metabolism of M. glaucescens after the induction of shoot organogenesis. These results help to clarify the molecular genetics and functional genomic aspects underlying propagation in the Cactaceae family.

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The YABBY Family Transcription Factor AaYABBY5 Directly Targets Cytochrome P450 Monooxygenase (CYP71AV1) and Double-Bond Reductase 2 (DBR2) Involved in Artemisinin Biosynthesis in Artemisia Annua

Cited by 33 publications

References 54 publications

OsNAC109 regulates senescence, growth and development by altering the expression of senescence- and phytohormone-associated genes in rice

OsNAC109 regulates senescence, growth and development by altering the expression of senescence- and phytohormone-associated genes in rice

Genome-wide identification and analysis of the YABBY gene family in Moso Bamboo (Phyllostachys edulis (Carrière) J. Houz)

Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

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