The WRKY53 transcription factor enhances stilbene synthesis and disease resistance by interacting with MYB14 and MYB15 in Chinese wild grape

Wang, Dan; Jiang, Cuiling; Liu, Wandi; Wang, Yuejin

doi:10.1093/jxb/eraa097

Cited by 49 publications

(44 citation statements)

References 89 publications

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“…We then used a yeast one-hybrid (Y1H) assay to examine the ability of VqMYB154 to bind to these motifs. VqMYB14 and VqMYB15 from Danfeng-2, which have been proven to promote the expression of STS genes 50 , were used for comparison. The results indicated that VqMYB154 is able to bind to the L5-box and AC-box motifs but not the MYBCORE motif, which is consistent with the results for VqMYB14 but not VqMYB15 (Fig.…”

Section: Resultsmentioning

confidence: 99%

VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine

et al. 2021

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Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accession Danfeng-2 is strongly expressed under artificial inoculation with Uncinula necator and regulates resveratrol accumulation. Unlike its homolog, VqMYB154 has a pathogen-induced promoter and responds to stimulation by U. necator, Pseudomonas syringae, and other treatments. Yeast one-hybrid and GUS activity assays confirmed that VqMYB154 can activate the stilbene synthase genes VqSTS9, VqSTS32, and VqSTS42 by directly binding to their promoters. Overexpression of VqMYB154 in grape leaves resulted in activation of the stilbene pathway, upregulation of STS genes, and accumulation of stilbenoids. In addition, heterologous overexpression of VqMYB154 in Arabidopsis activated resistance-related genes and resulted in greater programmed cell death and accumulation of reactive oxygen species, which led to resistance against P. syringae. These results suggest that the transcription factor VqMYB154 from V. quinquangularis accession Danfeng-2 participates in the regulatory mechanism that improves the biosynthesis and accumulation of stilbenes and enhances resistance to disease in grapevine.

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Section: Resultsmentioning

confidence: 99%

VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine

et al. 2021

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“…In recent years, the accumulation of resveratrol induced by biotic and abiotic stresses, such as pathogen attack and plant hormones, has been demonstrated by many studies (Belhadj et al, 2008;Schnee et al, 2008;D'Onofrio et al, 2009). WRKY, MYB, bZIP, and ERF transcription factors have been involved in regulating STS genes to increase the resveratrol content (Riccio et al, 2020;Wang et al, 2020). In addition, PRXs genes also have been reported to participate in regulating stilbene synthesis (Park et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry

Feng

Zhou

et al. 2021

Front. Plant Sci.

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Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape berries of ‘Summer Black’ and its bud sport ‘Nantaihutezao’ from the veraison to ripening stages using ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS). We found that bud sport accumulates the trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages but decreases its contents in the later stages. Simultaneously, we used RNA-Seq to identify 51 transcripts involved in the stilbene pathways. In particular, we further identified 124 and 19 transcripts that negatively correlated with the contents of trans-resveratrol and cis-resveratrol, respectively, and four transcripts encoding F3'5'H that positively correlated with the contents of trans-resveratrol by weighted gene co-expression network analysis (WGCNA). These transcripts may play important roles in relation to the synergistic regulation of metabolisms of resveratrol. The results of this study can provide a theoretical basis for the genetic improvement of grapes.

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“…Three WRKY transcription factor genes are present among the common MYB14/MYB15 HCTs. WRKY TFs have been suggested as co-regulators of MYB action in the control of STS expression [ (Wang et al, 2020), (Xi et al, 2014), (Xu et al, 2019)]. In fact, WRKY03 is bound by both TFs and supported in all three MYB14-, MYB15-and WRKY03-aggGCCNs.…”

Section: High Confidence Targets Integrated From Agggccns and Dap-seqmentioning

confidence: 99%

“…In fact, at least nine WRKY transcription factor genes are present among the common MYB14 /15 HCTs. WRKY TFs have been suggested as MYB co-regulators in the control of STS expression [ (Wang et al, 2020), (Xi et al, 2014), (Xu et al, 2019)] and also in many plant defence responses to pathogens. Particularly, WRKY03 is bound by both TFs and present in MYB14 and MYB15 leaf and fruit GCNs.…”

Section: Shared and Exclusive Regulatory Features Of S2 Myb Transcription Factorsmentioning

confidence: 99%

“…Nevertheless, the systems biology approaches initially conducted in (Wong et al, 2016), applied to the regulation of transcription in grapevine, have paved the way for the functional characterisation of additional stilbene pathway regulators such as bZIP1, ERF114, MYB35A and WRKY53 in recent years (Wang et al, 2019; Wang and Wang, 2019; Vannozzi et al, 2018), proving their efficacy in hypothesis driven research for TF discovery. Very interestingly, one of the newly identified stilbene regulators, WRKY53, binds to a subset of STS genes ( STS32 and STS41 ) and it is thought to form a regulatory complex with MYB14 and MYB15 probably increasing its activity (Wang et al, 2020). Moreover, WRKY03 has also been shown to work in synergy with MYB14 in the upregulation of STS29 expression (Vannozzi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Major orchestration of shikimate, early phenylpropanoid and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

Orduña

Navarro-Payá

et al. 2021

Preprint

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1AbstractThe control of plant specialised metabolism is exerted by transcription factors and co-regulators acting on cis-regulatory DNA sequences of pathway-structural genes, determining when, where, and how metabolites are accumulated. A particularly interesting case for studying the transcriptional control of metabolism is represented by stilbenoids, produced within the phenylpropanoid pathway, as their ability to inhibit infection by coronaviruses MERS-CoV and SARS-CoV has been recently demonstrated in vitro. Integrative omic studies in grapevine (Vitis vinifera L.), including gene co-expression networks, have previously highlighted several transcription factors (TFs) from different gene families as potential modulators of stilbenoid accumulation, offering an ideal framework for gene function characterisation using genome-wide approaches. In the context of non-model plant species, DNA affinity purification sequencing (DAP-Seq) results a novel and potentially powerful tool for the analysis of novel uncharacterised regulators, however, it has not yet been applied in fruit crops. Accordingly, we tested as a proof-of-concept the binding of two previously characterised R2R3-MYB TFs to their known targets of the stilbene pathway, MYB14 and MYB15, obtaining 5,222 and 4,502 binding events assigned to 4,038 and 3,645 genes for each TF, respectively. Bound genes (putative targets) were overlapped with aggregated gene centred co-expression networks resulting in shared and exclusive High Confidence Targets (HCTs) suggesting a high, but not complete, redundancy. Our results show that in addition to the previously known but few STS targets, these regulators bind to almost half of the complete STS family in addition to other phenylpropanoid- and stilbenoid-related genes. We also suggest they are potentially involved in other processes such as the circadian rhythm or the synthesis of biotin. We searched the activated transcriptomes of transiently MYB15-overexpressing grapevine plants and observed a large activation of its high confidence targets, validating our methodological approach. Our results also show that MYB15 seems to play a role in regulating other stilbenoid-related TFs such as WRKY03.

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The WRKY53 transcription factor enhances stilbene synthesis and disease resistance by interacting with MYB14 and MYB15 in Chinese wild grape

Cited by 49 publications

References 89 publications

VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine

VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine

Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry

Major orchestration of shikimate, early phenylpropanoid and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

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