The Wound-Healing Potential of Olea europaea L. Cv. Arbequina Leaves Extract: An Integrated In Vitro, In Silico, and In Vivo Investigation

Abstract

Olea europaea L. Cv. Arbequina (OEA) (Oleaceae) is an olive variety species that has received little attention. Besides our previous work for the chemical profiling of OEA leaves using LC–HRESIMS, an additional 23 compounds are identified. An excision wound model is used to measure wound healing action. Wounds are provided with OEA (2% w/v) or MEBO® cream (marketed treatment). The wound closure rate related to vehicle-treated wounds is significantly increased by OEA. Comparing to vehicle wound tissues, significant levels of TGF-β in OEA and MEBO® (p < 0.05) are displayed by gene expression patterns, with the most significant levels in OEA-treated wounds. Proinflammatory TNF-α and IL-1β levels are substantially reduced in OEA-treated wounds. The capability of several lignan-related compounds to interact with MMP-1 is revealed by extensive in silico investigation of the major OEA compounds (i.e., inverse docking, molecular dynamics simulation, and ΔG calculation), and their role in the wound-healing process is also characterized. The potential of OEA as a potent MMP-1 inhibitor is shown in subsequent in vitro testing (IC50 = 88.0 ± 0.1 nM). In conclusion, OEA is introduced as an interesting therapeutic candidate that can effectively manage wound healing because of its anti-inflammatory and antioxidant properties.

Apium extract alleviates indomethacin-induced gastric ulcers in rats via modulating the VEGF and IK-κB/NF-κB p65 signaling pathway: insights from in silico and in vivo investigations

“…The levels of GSH in gastric tissues exhibited a notable reduction subsequent to the administration of indomethacin. The observed decrease in GSH content could potentially be attributed to the generation of superoxide radicals, as these radicals have a tendency to deplete a significant quantity of endogenous antioxidant enzymes during scavenging [ 82 , 92 ]. The exacerbation of gastric mucosal damage can be attributed to the declined level of GSH, which leads to a rapid increase in hydrogen peroxide and lipid peroxides within the cells of the gastric mucosa.…”

Abelmoschus eculentus Seed Extract Exhibits In Vitro and In Vivo Anti-Alzheimer’s Potential Supported by Metabolomic and Computational Investigation

“…The extract was additionally concentrated using reduced pressure to give a syrupy consistency utilizing a rotary evaporator (Buchi Rotavapor R-300, Cole-Parmer, Vernon Hills, IL, USA), kept at 45 °C to afford 20.0 g crude extract. Moreover, this was maintained at 4 °C for biological and metabolomic studies [ 38 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ].…”

Vitis viniferaleaf extract liposomal Carbopol gel preparation's potential wound healing and antibacterial benefits:in vivo, phytochemical, and computational investigation

“…All the resulting fractions and the remaining crude extract were kept at 4 °C for biological and phytochemical investigations. 39–44…”

“…All the resulting fractions and the remaining crude extract were kept at 4 °C for biological and phytochemical investigations. [39][40][41][42][43][44] 2.5. Preparation of Vitis vinifera leaf extract liposomes (VVL-LP) VVL-LP was prepared by the thin film hydration method combined with hot sonication for reduction of the vesicle size and improved homogeneity.…”