The wound healing effects of the Tilapia collagen peptide mixture TY001 in streptozotocin diabetic mice

Abstract

BACKGROUND The Tilapia collagen peptides mixture TY001 is effective in promoting wound healing in acetic acid‐induced skin lesions in zebrafish and in protecting against lipopolysaccharide‐induced inflammation and disruption of glucose metabolism in mice. The present study aimed to further examine the wound healing effects of TY001 in streptozotocin‐induced diabetic mice. METHODS Full‐thickness skin excision wounds were created with 8‐mm biopsy punches and TY001 was administered via drinking water (15, 30 and 45 g L−1 in emulsion) for 15 days. RESULTS Wound healing was delayed in diabetic mice but was promoted by TY001 after 5, 10 or 15 days of treatment. Collagen deposition and tissue hydroxyproline contents were increased by TY001. The expressions of insulin growth factor‐1, basic fibroblast growth factor, platelet‐derived growth factor, transforming growth facts β1, vascular endothelial growth factor and epidermal growth factor were increased by TY001, as indicated by immunobiochemistry and a quantitative polymerase chain reaction. Diabetes‐associated serum pro‐inflammatory cytokines interleukin (IL)‐1β and IL‐8 were decreased, whereas anti‐inflammatory IL‐10 and nitric oxide were increased by TY001, along with increased tissue antioxidant superoxide dismutase and catalase activities. Diabetes‐reduced serum protein levels were also recovered by TY001 CONCLUSION Taken together, Tilapia collagen peptide mixture TY001 was effective with respect to enhancing diabetes‐associated wound healing delay, probably via increasing growth factors and collagen deposition in the wound, attenuating diabetes‐induced prolonged inflammation, increasing tissue antioxidants and providing nutritional support in diabetic mice. © 2019 Society of Chemical Industry

Tilapia Skin Peptides Ameliorate Diabetic Nephropathy in STZ-Induced Diabetic Rats and HG-Induced GMCs by Improving Mitochondrial Dysfunction

“…The tilapia skin collagen polypeptide alleviated liver and kidney injuries in mice induced by D-galactose [ 30 ]. The tilapia collagen peptide mixture TY001 improved glucose metabolism and reduced inflammation in STZ-induced diabetic mice [ 31 ]. However, a limited number of studies focused on the effect of marine-derived peptides on DN.…”

Protective Effects of Small-Molecule Oligopeptides Isolated from Tilapia Fish Scale on Ethanol-Induced Gastroduodenal Injury in Rats

“…Fish provides a rich source of collagen with high nutritional quality and is an ideal source for the preparation of collagen peptides [ 17 , 18 ]. Many studies have shown that bioactive peptides derived from Tilapia have various unique physiological functions, such as antioxidation, anti-aging, lowering blood pressure, immune regulation, antibacterial activity, and the ability to repair tissue defects [ 19 , 20 , 21 , 22 ]. Furthermore, tilapia collagen oligopeptides (TCOPs) extracted from tilapia have lower molecular weight, better digestibility and absorption [ 19 , 23 ], and great value in the prevention and treatment of gastroduodenal injury.…”

Collagen-Derived Di-Peptide, Prolylhydroxyproline (Pro-Hyp): A New Low Molecular Weight Growth-Initiating Factor for Specific Fibroblasts Associated With Wound Healing

“…Pro-Hyp is crucial for the proliferation of p75NTR-positive fibroblasts cultured on collagen gel. Some animal studies have also demonstrated that the oral administration of fish gelatin and collagen hydrolysate improved the delayed wound healing in diabetic rats and mice, respectively (Zhang et al, 2011 ; Xiong et al, 2020 ). Therefore, supplementation of collagen hydrolysate or gelatin has therapeutic potential for chronic wounds in diabetic patients.…”