The wound healing effect of collagen/adipose‐derived stem cells (ADSCs) hydrogel: In vivo study

Abstract

Background The complex wound healing process involves activating and synchronizing intracellular, intercellular, and extracellular components. Adipose tissue is attracting attention to promote wound healing. Within subcutaneous adipose tissue, stromal vascular cells and their subsets release growth factors and cytokines critical for neovascularization and wound repair. Objectives This study evaluated human placental collagen/adipose‐derived stem cells (ADSCs) hydrogel for wound healing in rats. Methods In this study, ADSCs were harvested, cultured, and mixed with placental collagen. Twelve rats were used, and their backs were excised three times each. Group one received collagen/ADSCs, group two collagen, and group three non‐filled (control) excisions. The healing processes were assessed by histological analysis, taking photographs, and calculating the percentage of wound contraction in mentioned times. Results Histopathological analysis revealed that the content of fibroblasts, follicles of the hair, and angiogenesis in group one was significantly more than in other groups. Group one had a significant result compared with the collagen and control groups. In group one, significant wound healing and wound contraction were observed with 52% and 80% wound contraction at 7 and 14 days, respectively. Conclusion Collagen/ADSCs can be considered a suitable candidate hydrogel in wound healing with a high potential for enhancing wound repairing.

Bevacizumab, a vascular endothelial growth factor inhibitor, promotes orthodontic tooth movement in an experimental rat model

“…In agreement with our study, inhibition of VEGF by bevacizumab downregulated the expression of Col-I in primary human alveolar osteoblasts, which was postulated by the authors of the previous study as a potential explanation for bevacizumab-associated MRONJ [ 28 ]. Moreover, studies showed that enhanced angiogenesis is associated with higher collagen production, especially the type I collagen (Col-I) [ 34 ]. Accordingly, restricted angiogenesis can be anticipated as a plausible cause for the reduced Col-I production observed in the present study.…”

Signaling Pathways Triggering Therapeutic Hydrogels in Promoting Chronic Wound Healing

“…Adult stem cells, such as mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs), can secrete kinds of essential cytokines that are beneficial to wound healing, are widely used as cell therapy. [89][90][91][92][93] Hydrogels, owing to their porous structure and moisture-retaining properties, serve as optimal carriers for cell transplantation. For instance, Wu et al engineered a ECM-like hydrogel with hyaluronic acid methacryloyl (HAMA) and fibronectin molecule chain (FN-HAMA) to encapsulate ADSCs for diabetic wound healing (Figure 2D).…”

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

“…Meanwhile, stem cells, particularly adipose-derived stem cells (ADSCs), have shown great promise in tissue engineering and regenerative medicine due to their unique properties. − Since ADSCs are easy to access, are abundant, and have multilineage plasticity, they have emerged as a promising skin regeneration candidate . They secrete various growth factors that promote wound healing, making them ideal for enhancing tissue renewal. , Although ADSCs have the potential to differentiate into functional keratinocytes, their efficient and controlled differentiation remains a challenge …”

“…11 They secrete various growth factors that promote wound healing, making them ideal for enhancing tissue renewal. 12,13 Although ADSCs have the potential to differentiate into functional keratinocytes, their efficient and controlled differentiation remains a challenge. 14 Some growth factors, such as epithelial growth factor (EGF), 15 keratinocyte growth factor (KGF), 16 fibroblast growth factor (FGF), 17 and hepatocyte growth factor (HGF), 18 into keratinocyte-like cells.…”