The Wnt/PCP formin Daam1 drives cell-cell adhesion during nephron development

Abstract

Highlights d Daam1 localizes to cell-cell contacts in developing nephron d Daam1 regulates the cytoskeletal membrane dynamics d Daam1 promotes E-cadherin localization d Formin homology domain 2 (FH2) of Daam1 mediates Ecadherin localization

A comparative study of cellular diversity between the Xenopus pronephric and mouse metanephric nephron

“…Given that seven cell clusters were identified in our primary analyses of cells in the Xenopus pronephros, a lower number than that identified in the mouse, cell identities from the mouse kidney cell atlas were grouped as follows: (Fig. S3C, 5D): Podocytes (1) Parietal Epithelium (2) Proximal Tubule (3)(4)(5)(6)(7)(8) Loop of Henle (9a-14) Distal Tubule (15)(16)(17)(18)(19) Principal cells (20,23,26,28,29) Intercalated cells (21,22,24,25) Duct cells (30)(31)(32). For all the genes enriched in each Xenopus segment, the average expression of these genes in mouse was calculated.…”

“…The Xenopus pronephric kidney consists of a single nephron 14 , which is visible through the organism's semitransparent epidermis. This kidney's simplicity enables visualization of processes essential to kidney development and in vivo imaging [15][16][17] .…”

Planar cell polarity regulators in asymmetric organogenesis during development and disease

“…In Xenopus and zebrafish pronephros, Daam1 may function through Rho GTPase to regulate cytoskeletal rearrangements for proper tubulogenesis and tubule morphology (Miller et al, 2011). It also colocalizes with E-cadherin at cell-cell contact regions in the Xenopus nephric primordium to ensure intercellular adhesion and epithelial tissue organization during CE movements (Krneta-Stankic et al, 2021). In mice, Fuz appears to regulate ureteric bud branching morphogenesis through cilia-dependent and -independent pathways, raising the possibility that it may play a role in ciliogenesis (Wang et al, 2021).…”

A comparative study of cellular diversity between the Xenopus pronephric and mouse metanephric nephron

“…Given that seven cell clusters were identified in our primary analyses of cells in the Xenopus pronephros, a lower number than that identified in the mouse, cell identities from the mouse kidney cell atlas were grouped as follows: (Fig. S3C, 5D): Podocytes (1) Parietal Epithelium (2) Proximal Tubule (3)(4)(5)(6)(7)(8) Loop of Henle (9a-14) Distal Tubule (15)(16)(17)(18)(19) Principal cells (20,23,26,28,29) Intercalated cells (21,22,24,25) Duct cells (30)(31)(32). For all the genes enriched in each Xenopus segment, the average expression of these genes in mouse was calculated.…”

“…The Xenopus pronephric kidney consists of a single nephron 14 , which is visible through the organism’s semitransparent epidermis. This kidney’s simplicity enables visualization of processes essential to kidney development and in vivo imaging 15–17 . Additionally, the ability to quickly knock out, knock down, or overexpress genes in Xenopus has facilitated a mechanistic understanding of kidney development.…”