The winged-helix transcription factor JUMU regulates development, nucleolus morphology and function, and chromatin organization of Drosophila melanogaster

Hofmann, Annemarie; Brünner, Madeleine; Schwendemann, Alexander; Strödicke, Martin; Karberg, Sascha; Klebes, Ansgar; Saumweber, Harald; Korge, Günter

doi:10.1007/s10577-010-9118-y

Cited by 10 publications

(12 citation statements)

References 54 publications

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“…Moreover, localization studies for Delta (Dl) and Ecdysone Receptor (EcR) show a precise colocalization of EGFP and the protein specific antibodies for Delta and EcR, respectively ( Figure 2—figure supplement 1 ). Finally, proteins localize to the proper subcellular compartments as shown in Figure 2B for a cytoplasmic or organelle present in cytoplasm associated ( Catalase ), nuclear ( H6-like-homeobox : Hmx ) and membrane associated protein ( dpr15 ) ( Figure 2B ) ( Beard and Holtzman, 1987 ; Hofmann et al, 2010 ; Özkan et al, 2013 ). These examples indicate that the EGFP patterns faithfully report expression and protein distribution.…”

Section: Resultsmentioning

confidence: 99%

A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Nagarkar-Jaiswal

Lee

Campbell

et al. 2015

eLife

360

371

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Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.DOI: http://dx.doi.org/10.7554/eLife.05338.001

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Section: Resultsmentioning

confidence: 99%

A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Nagarkar-Jaiswal

Lee

Campbell

et al. 2015

eLife

360

371

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“…It has been demonstrated that Jumu regulates nucleolar morphology and function and chromatin organization and that the correct genetic dose is required for nucleolar integrity and correct nucleolar function (Hofmann et al, 2010). Here, we found that Jumu also regulates the hemocyte differentiation of the lymph gland in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Dual role for Jumu in the control of hematopoietic progenitors in the Drosophila lymph gland

Hao

Jin

2017

eLife

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The Drosophila lymph gland is a hematopoietic organ in which the maintenance of hematopoietic progenitor cell fate relies on intrinsic factors and extensive interaction with cells within a microenvironment. The posterior signaling center (PSC) is required for maintaining the balance between progenitors and their differentiation into mature hemocytes. Moreover, some factors from the progenitors cell-autonomously control blood cell differentiation. Here, we show that Jumeau (Jumu), a member of the forkhead (Fkh) transcription factor family, controls hemocyte differentiation of lymph gland through multiple regulatory mechanisms. Jumu maintains the proper differentiation of prohemocytes by cell-autonomously regulating the expression of Col in medullary zone and by non-cell-autonomously preventing the generation of expanded PSC cells. Jumu can also cell-autonomously control the proliferation of PSC cells through positive regulation of dMyc expression. We also show that a deficiency of jumu throughout the lymph gland can induce the differentiation of lamellocytes via activating Toll signaling.DOI: http://dx.doi.org/10.7554/eLife.25094.001

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“…Mutations in these genes lead to haplo-insufficient phenotypes of delayed development, shortened and thin bristles, and reduced viability and fertility. Other Drosophila genes known to encode nucleolar processing and/or assembly factors have also been characterized in terms of their mutant phenotype; those that have been better characterized include minifly , 197 modulo , 198 - 200 viriato , 201 dribble , 202 jumeaux , 203 pitchoune , 204 and Nopp140 205 , 206 . The minifly gene (also called Nop60B ) encodes a pseudouridylase homologous to human DCK1 .…”

Section: Endogenous Mutations: the Ribosomopathiesmentioning

confidence: 99%

Nucleolar stress with and without p53

James

Wang

Raje

et al. 2014

Nucleus

237

280

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A veritable explosion of primary research papers within the past 10 years focuses on nucleolar and ribosomal stress, and for good reason: with ribosome biosynthesis consuming ~80% of a cell’s energy, nearly all metabolic and signaling pathways lead ultimately to or from the nucleolus. We begin by describing p53 activation upon nucleolar stress resulting in cell cycle arrest or apoptosis. The significance of this mechanism cannot be understated, as oncologists are now inducing nucleolar stress strategically in cancer cells as a potential anti-cancer therapy. We also summarize the human ribosomopathies, syndromes in which ribosome biogenesis or function are impaired leading to birth defects or bone narrow failures; the perplexing problem in the ribosomopathies is why only certain cells are affected despite the fact that the causative mutation is systemic. We then describe p53-independent nucleolar stress, first in yeast which lacks p53, and then in other model metazoans that lack MDM2, the critical E3 ubiquitin ligase that normally inactivates p53. Do these presumably ancient p53-independent nucleolar stress pathways remain latent in human cells? If they still exist, can we use them to target >50% of known human cancers that lack functional p53?

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The winged-helix transcription factor JUMU regulates development, nucleolus morphology and function, and chromatin organization of Drosophila melanogaster

Cited by 10 publications

References 54 publications

A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Dual role for Jumu in the control of hematopoietic progenitors in the Drosophila lymph gland

Nucleolar stress with and without p53

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