The wilt pathogen induces different variations of root-associated microbiomes of plant

Abstract

Root-associated compartments, including the rhizosphere, rhizoplane, and endosphere, live with diverse microbial communities which profoundly affect plant growth and health. However, a systematic understanding of the microbiome assembly across the rhizosphere, rhizoplane, and endosphere under pathogen invasion remains elusive. Using 16S high-throughput sequencing, we studied how bacterial wilt disease affected the variation and assembly of the three continuous root-associated microbiomes of tobacco. The results indicated that microorganisms were gradually filtered from the rhizosphere to the endosphere. With the pathogen invasion, the rhizosphere, rhizoplane and endosphere microbiomes selected and recruited different beneficial bacterial taxa. Some recruited bacteria were also identified as keystone members in networks (i.e., Bosea in the endosphere). The microbiomes of endosphere and rhizoplane were more sensitive to plant disease than the rhizosphere microbiome. Still, response strategies of the rhizoplane and endosphere to disease were obviously different. Microbial networks of the rhizoplane became complex in diseased samples and genes involved in sporulation formation and cell cycle were enriched. However, microbial networks of the diseased endosphere were disrupted, and functional genes related to nitrogen utilization and chemotaxis were significantly increased, indicating the importance of nitrogen resources supply of plants for the endosphere microbiome under pathogen invasion. Our results provide novel insights for understanding the different responses of the root-associated microbiomes to plant disease.

Responses of the bacterial community of tobacco phyllosphere to summer climate and wildfire disease

“…This indicated that T2 was more complex, with abundant interactions in a highly connected microbial community, which might also be explained by the rainfall peak at T2 that has provided great growth opportunity for microbes to multiply and make connections as mentioned above. A similar phenomenon of relatively higher connections was also observed in the wilt diseased rhizoplane of tobacco (Tao et al, 2022) . The percentage of positive correlation follow largely a downtrend from T1 (57.4%) to T3 (53.6%), indicating the decrease of cooperative relation.…”

Effects of long‐term mowing on leaf‐ and root‐associated bacterial community structures are linked to functional traits in 11 plant species from a temperate steppe

“…For example, Xiong, He, et al (2021); Xiong, Zhu, et al (2021) found that soil is the main source of microorganisms colonizing the root compartments, which are then filtered and enriched in the leaf compartments, along the soil–plant continuum. However, recent studies suggest that the major sources of microbiomes in specific compartments may be altered by disturbances (Dove et al, 2021; Sun et al, 2022) or pathogen infections (Dastogeer et al, 2022; Tao et al, 2022). Therefore, it is important to investigate whether mowing can shift the sources of leaf and root microbiomes and elucidate the mechanisms underlying the recruitment of microbes by plant compartments under mowing (Sun et al, 2022).…”

Phyllosphere bacterial community dynamics in response to bacterial wildfire disease: succession and interaction patterns

“…(2020) revealed that the rhizosphere and endophytic compartments of infected tobacco were more complex compared to those of healthy tobacco. Similarly, a higher level of connections was also observed in the wilt-diseased rhizoplane of tobacco compared to healthy samples ( Ahmed et al., 2022 ; Tao et al., 2022 ).…”