The wide spectrum of methods available to study marine neurotoxins

Campàs, Mònica; Alkassar, Mounira; Gaiani, Greta; Leonardo, Sandra; Rambla-Alegre, Maria; Diogène, Jorge

doi:10.1016/bs.ant.2021.03.005

Cited by 7 publications

(5 citation statements)

References 177 publications

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“…In what follows, bioassays, cell-based assays (CBAs), immunoassays, aptamer-based assays, biosensors, and instrumental analysis techniques for TTXs are summarized. Further details can be found in the mentioned bibliographic references and in more specific methodological reviews [3,9,27,[276][277][278][279].…”

Section: Analytical Methods For the Detection And Quantification Of Ttxsmentioning

confidence: 99%

An Updated Review of Tetrodotoxin and Its Peculiarities

et al. 2022

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Tetrodotoxin (TTX) is a crystalline, weakly basic, colorless organic substance and is one of the most potent marine toxins known. Although TTX was first isolated from pufferfish, it has been found in numerous other marine organisms and a few terrestrial species. Moreover, tetrodotoxication is still an important health problem today, as TTX has no known antidote. TTX poisonings were most commonly reported from Japan, Thailand, and China, but today the risk of TTX poisoning is spreading around the world. Recent studies have shown that TTX-containing fish are being found in other regions of the Pacific and in the Indian Ocean, as well as the Mediterranean Sea. This review aims to summarize pertinent information available to date on the structure, origin, distribution, mechanism of action of TTX and analytical methods used for the detection of TTX, as well as on TTX-containing organisms, symptoms of TTX poisoning, and incidence worldwide.

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Section: Analytical Methods For the Detection And Quantification Of Ttxsmentioning

confidence: 99%

An Updated Review of Tetrodotoxin and Its Peculiarities

et al. 2022

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“…The size and structure of the target analyte play an important role in immunosensing configuration [90]. With low-molecular weight antigens, such as TTXs, competitive formats are usually required.…”

Section: Immunosensorsmentioning

confidence: 99%

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

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Alkassar

Diogène

et al. 2023

Foods

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The emergence of marine toxins such as ciguatoxins (CTXs) and tetrodotoxins (TTXs) in non-endemic regions may pose a serious food safety threat and public health concern if proper control measures are not applied. This article provides an overview of the main biorecognition molecules used for the detection of CTXs and TTXs and the different assay configurations and transduction strategies explored in the development of biosensors and other biotechnological tools for these marine toxins. The advantages and limitations of the systems based on cells, receptors, antibodies, and aptamers are described, and new challenges in marine toxin detection are identified. The validation of these smart bioanalytical systems through analysis of samples and comparison with other techniques is also rationally discussed. These tools have already been demonstrated to be useful in the detection and quantification of CTXs and TTXs, and are, therefore, highly promising for their implementation in research activities and monitoring programs.

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“…Historically, animal bioassays, such as the mouse bioassay (MBA), were the primary methods for the toxicological characterization of marine toxins . However, MBA is now of limited use in many countries due to its low specificity, high variability and the ethical issues concerning the experimentation with live animals . Cell-based assays (CBAs) have been proposed as an alternative approach to assess the toxicity of a sample without involving live animals.…”

Section: Introductionmentioning

confidence: 99%

“…13 However, MBA is now of limited use in many countries due to its low specificity, high variability and the ethical issues concerning the experimentation with live animals. 24 Cell-based assays (CBAs) have been proposed as an alternative approach to assess the toxicity of a sample without involving live animals. The detection of toxins with CBA relies on assessing changes in the morphology or viability of cells after their exposure to them.…”

Section: Introductionmentioning

confidence: 99%

Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells

Reverté,

Rambla-Alegre,

Sanchez-Henao

et al. 2024

J. Agric. Food Chem.

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Tetrodotoxin (TTX) is a potent marine neurotoxin, responsible for numerous poisoning incidents and some human fatalities. To date, more than 30 TTX analogues have been identified, but their individual toxicities and roles in poisoning remain largely unknown. In this work, the toxicity equivalency factors (TEFs) of five TTX analogues were determined by assessing the blockade of voltage-gated sodium channels in Neuro-2a cells using automated patch clamp (APC). All TTX analogues were less toxic than TTX. The derived TEFs were applied to the individual TTX analogues concentrations measured in pufferfish samples, using liquid chromatography coupled to tandem mass spectrometry (LC−MS/MS). A comparison of these results with those obtained from APC analysis demonstrated that TEFs can be effectively used to translate LC−MS/MS analytical data into meaningful toxicological information. This is the first study to utilize APC device for the toxicological assessment of TTX analogues, highlighting its potential as a bioanalytical tool for seafood safety management and human health protection.

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The wide spectrum of methods available to study marine neurotoxins

Cited by 7 publications

References 177 publications

An Updated Review of Tetrodotoxin and Its Peculiarities

An Updated Review of Tetrodotoxin and Its Peculiarities

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells

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